Optimization of the Separation of Quinolines in Micellar Liquid Chromatography by Experimental Design and Regression Models

Abstract

The chemometrics approach was applied to the optimization of separation of quinolines in micellar liquid chromatography (MLC). The effect of four experimental parameters on chromatographic retention time was investigated by means of multivariate analysis. The factors considered were the concentration of sodium dodecyl sulfate (SDS), the organic modifier concentration and the length of its alkyl chain, and pH of the mobile phase. The experiments were performed according to a face centered cube response surface experimental design. In order to optimize the separation a Pareto-optimality method was employed. The models were verified, because a good agreement was observed between the predicted and experimental values of the chromatographic response function in the optimal condition. The obtained regression models were characterized by both descriptive and predictive ability (R 2 ≥0.97 and 2 cv R ≥0.92) and allowed the chromatographic separation of the quinolines with a good resolution and a total analysis time of 50 min.

Extraction of juglone from Pterocarya fraxinifolia leaves for dyeing, anti‐fungal finishing, and solar UV protection of wool

“…A peak that appeared at 237 nm was attributable to π–π* transitions, whereas other absorptions at 407 and 667 nm in the visible region could be due to n –π* transitions of the carbonyl groups in juglone. These peaks were previously demonstrated by Hadjmohammadi and Kamel . Natural dyes extracted from plants generally absorb visible or UV light by transferring the electrons from the highest occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO).…”

“…The leaves are large, thick, attractive, dark green, and free of pests. Juglone (5‐hydroxy‐1,4‐naphthoquinone) is the main compound present in the leaves .…”

Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

“…low toxicity, low cost, low volatility of mobile phase constituents, the possibility of simultaneous separation of ionic and non-ionic compounds. Moreover, it offers different separation selectivity, when compared to other chromatographic methods, owing to the involvement of a large number of parameters [7].…”

“…SDS is a most adequate surfactant for use in the analysis of compounds with basic functional groups, such as quinolines [7], Fluconazole and Tinidazole, in pharmaceuticals and biological fluids [2], as well as with Timolol maleate in the presence of its degradation products [11].…”

A Validated Micellar Lc Method For Simultaneous Determination of Furosemide, Metoprolol And Verapamil in Human Plasma

“…In addition because of the divers interaction between analyte and mobile phase it is possible to separate a divers set of analytes with different pKa and logP values. MLC have been applied for ana lysis of diuretics [18], b-blockers [19], anticonvulsants [20], verapamil [3], quinolines [21], tamoxifen [22], disopyramide, lidocaine and quinidine [23].…”