Optimization of the separation of a complex mixture of natural and synthetic anabolic steroids by micellar liquid chromatography

Izquierdo-Hornillos, R.; Gonzalo-Lumbreras, R.

doi:10.1016/j.jchromb.2003.08.050

Cited by 10 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An LC separation of a complex mixture containing 14 androgenic anabolic steroids (natural and synthetic) for anabolic steroid screening purposes has been carried out. The optimization of the method assessed the use of binary, ternary and quaternary mobile phases containing acetonitrile, methanol or tetrahydrofuran as organic modifiers (Izquierdo-Hornillos and Gonzalo-Lumbreras, 2003). The effects of different reversed-phase packings, and temperature on the separation using acetonitrile as organic modifier were also studied.…”

Section: Mobile Phasesmentioning

confidence: 99%

General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

Makin

Honour

Shackleton³

et al. 2010

Steroid Analysis

View full text Add to dashboard Cite

Section: Mobile Phasesmentioning

confidence: 99%

General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

Makin

Honour

Shackleton³

et al. 2010

Steroid Analysis

View full text Add to dashboard Cite

Complete chromatographic separation of steroids, including 17α and 17β-estradiols, using a carbazole-based polymeric organic phase in both reversed and normal-phase HPLC

Mallik¹,

Shingo²,

Gautam³

et al. 2010

Anal Bioanal Chem

View full text Add to dashboard Cite

Poly(2-N-carbazolylethyl acrylate) with terminal trimethoxysilyl groups was prepared as an organic phase and immobilized onto silica. The retention behavior of the column packed with this carbazole-based polymer-immobilized silica (Sil-CEA) was investigated by using various estrogenic steroids and corticoids in both reversed-phase and normal-phase liquid chromatography. As a result, complete separation was confirmed for eight kinds of steroids with Sil-CEA. The most specific separation with Sil-CEA can be emphasized by the high separation factor (e.g., alpha = 1.39 in methanol-water (7:3, v/v) at 35 degrees C) for 17alpha and 17beta-estradiols, one of the most difficult pairs of isomers in chromatographic separation, whereas for two kinds of commercially available polymeric ODS columns as references alpha = 1.01, only, under the same conditions. Because the excellent separation and retention order with Sil-CEA was maintained even in a normal-phase mobile phase such as a hexane-2-propanol, it is estimated that the CEA phase has multiple interaction mechanisms through stronger interactions such as dipole-dipole, carbonyl-pi, and hydrogen bonding interactions than the hydrophobic effect expected with ODS.

show abstract

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

et al. 2012

View full text Add to dashboard Cite

A novel alternating copolymer-based organic phase was synthesized using a new N-substituted maleimide monomer for the development of alternating copolymer-grafted silica for high-performance liquid chromatographic applications. This new monomer (DGMI) was copolymerized with octadecyl acrylate (ODA) from 3-mercaptopropyltrimethoxysilane-grafted silica to produce Sil-poly(ODA-alt-DGMI). The organic phase was characterized by the elemental analysis and the diffuse reflectance infrared Fourier transform spectroscopy. Tocopherol isomers and steroids were used as analytes for the evaluation of the chromatographic selectivity profiles of this novel stationary phase. The selectivity of this column was then compared with a polymeric ODS column and previously developed another alternating copolymer-grafted silica (without the glutamide-derived moiety) column, Sil-poly(ODA-alt-N-octadecylmaleimide). The complete baseline separation of tocopherol isomers in an isocratic mode has been achieved within 25 min with the Sil-poly(ODA-alt-DGMI). The separation of eight kinds of estrogenic steroids and corticoids has also been achieved in an isocratic mode with this column. Significant differences in separation selectivity between Sil-poly(ODA-alt-DGMI) and polymeric ODS columns were observed towards the steroids, and compared with the reference columns, a better separation profile for these analytes was obtained with the Sil-poly(ODA-alt-DGMI). The results of this investigation indicated that the enhancement of selectivity of Sil-poly(ODA-alt-DGMI) towards the test analytes arose from the multiple interaction mechanism such as hydrophobic effect, carbonyl-π and hydrogen-bonding interactions, and such integrated interactions originated from the addition of two amide groups in the N-substituted maleimide monomer.

show abstract

Optimization of the separation of a complex mixture of natural and synthetic anabolic steroids by micellar liquid chromatography

Cited by 10 publications

References 21 publications

General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

Complete chromatographic separation of steroids, including 17α and 17β-estradiols, using a carbazole-based polymeric organic phase in both reversed and normal-phase HPLC

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

Contact Info

Product

Resources

About