Optimization of the separation of polycarboxylic acids by capillary zone electrophoresis

Buchberger, Wolfgang; Winna, Klemens

doi:10.1016/0021-9673(96)00056-8

Cited by 34 publications

(11 citation statements)

References 25 publications

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“…Most of these papers report the analysis of carboxylic acids with indirect UV detection [11 17, 19 24], or conductivity detection [16,17,21]; few papers describe the analysis of these acids by use of direct detection [18,25,26] and only the method of Saavedra et al [18] has been used with food samples. The aim of this work was optimization of a CE method with direct UV detection for separation and determi- …”

Section: Introductionmentioning

confidence: 99%

Determination of organic acids by capillary electrophoresis with simultaneous addition of Ca and Mg as complexing agents

2003

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SummaryA capillary electrophoretic method, with divalent cations as complexing agents in the electrolyte, has been developed for separation and determination of the low molecular weight organic acids most commonly found in wine, viz. formic, fumaric, succinic, oxalic, mahc, tartaric, acetic, lactic, and citric acids. The separation conditions optimized were electrolyte concentration, organic flow modifier concentration, type and concentration of complexing agents in the electrolyte, and injection time. The best resolution of some of the acids studied was achieved by use of an electrolyte containing tetraborate buffer (10 mM) at pH 9.3, an organic flow modifier (tetradecyltrimethylammonium hydroxide), and Ca 2+ (10 ppm) and Mg 2+ (10 ppm) as complexing agents. Other conditions used in the method were hydrostatic injection (10 cm height for 30 s), detection at 185 nm, and temperature 20 ~ For all the acids studied detector response was linear for the concentration ranges considered. The repeatability of each point on the calibration plot for standards (n = 4)was generally better than 1%. The method was applied to samples of must, wine, brandy, and vinegar from the Jerez region.

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Section: Introductionmentioning

confidence: 99%

Determination of organic acids by capillary electrophoresis with simultaneous addition of Ca and Mg as complexing agents

2003

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“…A typical example for such an approach is the addition of selective complexing agents, like divalent metal ions for dicarboxylic acids, allowing their separation from overlapping or coeluting monoacids. In principle all these modes of separation are well known and have been investigated extensively over the last 2-3 decades [10,11].…”

Section: Czementioning

confidence: 99%

Determination of organic acids by CE and CEC methods

Klampfl

2007

Electrophoresis

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A comprehensive overview of the analysis of low-molecular-mass organic acids employing electromigration methods in the capillary format is given. This review includes papers published since 2003 and can be seen as an update of the review paper published by Galli et al. in 2003. Tables included in this review contain application papers describing the determination of organic acids from a variety of fields like the analysis of food and beverages, environmental samples, samples from clinical origin, and from natural products.

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“…When this approach can be applied it is usually more specific and sensitive than indirect UV detection (Shirao et al, 1994;Turcat et al, 1994;Buchberger and Winna, 1996). Electrolytes based on non-absorbing species such as sulfate or phosphate are used.…”

Section: Sensitivity: Comparison Between Direct and Indirect Uv Detecmentioning

confidence: 99%

Analysis of Organic Acids in Wine by Capillary Electrophoresis with Direct UV Detection

Ventoso

Peña

Herrero

et al. 2002

Journal of Food Composition and Analysis

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Optimization of the separation of polycarboxylic acids by capillary zone electrophoresis

Cited by 34 publications

References 25 publications

Determination of organic acids by capillary electrophoresis with simultaneous addition of Ca and Mg as complexing agents

Determination of organic acids by capillary electrophoresis with simultaneous addition of Ca and Mg as complexing agents

Determination of organic acids by CE and CEC methods

Analysis of Organic Acids in Wine by Capillary Electrophoresis with Direct UV Detection

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