Optimization of ultrasonic-assisted extraction of continentalic acid from the root of Aralia continentalis by using the response surface methodology

Kim, Hyo Seon; Lee, A Yeong; Jo, Ji Eun; Moon, Byeong Cheol; Ji, Yunui; Kim, Ho Kyoung

doi:10.1007/s12272-013-0291-3

Cited by 6 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are in accordance with single factor test and the ANOVA analysis (Table 2). The results are also in agreement with reports by the authors reporting the extraction of continentalic acid from the root of Aralia continentalis [26].…”

Section: Resultssupporting

confidence: 93%

Optimization of Antifungal Extracts from Ficus hirta Fruits Using Response Surface Methodology and Antifungal Activity Tests

et al. 2015

View full text Add to dashboard Cite

Abstract:The fruits of Ficus hirta (FH) display strong antifungal activity against Penicillium italicum and Penicillium digitatum. In order to optimize the extraction conditions of antifungal extracts from FH fruit, various extraction parameters, such as ethanol concentration, extraction time, solvent to solid ratio and temperature, were chosen to identify their effects on the diameters of inhibition zones (DIZs) against these two Penicillium molds. Response surface methodology (RSM) was applied to obtain the optimal combination of these parameters. Results showed that the optimal extraction parameters for maximum antifungal activity were: 90% (v/v) ethanol concentration, 65 min extraction time, 31 mL/g solvent to solid ratio and 51˝C temperature. Under the abovementioned extraction conditions, the experimental DIZs values obtained experimentally were 57.17˘0.75 and 39.33˘0.82 mm, which were very close to the values of 57.26 and 39.29 mm predicted by the model. Further, nine kinds of phytopathogens were tested in vitro to explore the antifungal activity of the FH extracts. It was found for the first time that the FH extracts showed significant inhibition on the growth of P. italicum, A. citri, P. vexans, P. cytosporella and P. digitatum.

show abstract

Section: Resultssupporting

confidence: 93%

Optimization of Antifungal Extracts from Ficus hirta Fruits Using Response Surface Methodology and Antifungal Activity Tests

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The KA standard was purchased by the Korea Promotion Institute for Traditional Medicine Industry, while the CA standard has been previously isolated in our laboratory (Fig. ) . HPLC‐grade ethanol, methanol, acetonitrile, and water were purchased from J. T. Baker (Phillipsburg, NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

“…First, the powdered root of A. continentalis was sieved through a 50 mesh sieve. Second, 0.2 g of the powder was accurately weighed and added into a capped vial, followed by extraction with 10 mL of ethanol using an ultrasonication bath (model 8510, 44 kHz; Branson, Danbury, CT, USA) for 28 min . Next, the extracted solution was transferred to a 10 mL volumetric flask, the final volume was made up to 10 mL using ethanol, and the solution was then filtered through a 0.2 μm membrane disk filter before injection into the UPC 2 system.…”

Section: Methodsmentioning

confidence: 99%

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Kim

Moon

Choi

et al. 2017

J of Separation Science

Self Cite

View full text Add to dashboard Cite

A rapid ultra-performance convergence chromatography method was developed for the quantitative determination of bioactive compounds in Aralia continentalis as quality control markers. Quantitative analysis indicated the presence of two major bioactive compounds: diterpenoid acids continentalic acid and kaurenoic acid. Using a Torus 1-aminoanthracene column, continentalic acid and kaurenoic acid were separated in less than 8 min. The method was validated with respect to precision, accuracy, and linearity according to the International Conference on Harmonization guidelines. The optimized method exhibited a good linear correlation (r > 0.996), excellent precision (RSD< 1.0%), and acceptable recoveries (99.97-100.26%). Limits of detection for continentalic acid and kaurenoic acid were 0.068 and 0.097 μg/mL, respectively, while their corresponding limits of quantitation were 0.207 and 0.295 μg/mL. The system performance of ultra-performance convergence chromatography was compared with that of conventional high-performance liquid chromatography with respect to analysis time and efficiency. The proposed method was found to be reliable and convenient for the quantitative analysis of continentalic acid and kaurenoic acid in A. continentalis from South Korea and A. pubescens from China. This study is expected to serve as a guideline for the quality control of Aralia continentalis.

show abstract

“…The material-to-liquid ratio and extraction time significantly affected the Cers yield, and as time progressed, the material-to-liquid ratio contacted the sample fully and enhanced the interaction. A shorter ultrasonication time may result in an insignificant optimization effect, as shown by the slope steepness, elliptical shape, and density of the contour line, indicating that the material-to-liquid ratio exerted a significantly large effect on neural extraction, thereby, highlighting the importance of exploring the interactions between the material-to-liquid ratio and extraction time [28]. The effects of the interaction between extraction time and temperature on the Cers yield under a fixed material-to-liquid ratio of 5 g/mL are shown (Figure 3A).…”

Section: Response Surface Analysismentioning

confidence: 99%

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Wang

Jia

Song

et al. 2022

Foods

View full text Add to dashboard Cite

As a new type of salt-tolerant rice, sea red rice contains more minerals, proteins, and lipid compounds, and, in particular, its by-product rice bran may be used to replace other commercial rice brans as the main source of ceramides (Cers). However, the extraction rate of Cers is generally low, and it is crucial to seek an efficient extraction method. This study optimized the ultrasonic-assisted extraction of Cers from sea red rice bran using response surface methodology (RSM) and obtained a Cers yield of 12.54% under optimal conditions involving an extraction temperature of 46 °C, an extraction time of 46 min, and a material–to-liquid ratio of 5 g/mL. The Cers content in sea red rice bran was preliminarily analyzed using thin-layer chromatography, and the Cers content was determined via UHPLC-Triple-TOF-MS/MS after purification and separation using silica column chromatography. Forty-six different types of Cers were identified in sea red rice bran, of which Cer 18:0/24:0 (2OH), Cer 18:0/26:0, Cer 18:0/26:0 (2OH), and Cer 18:0/24:0 accounted for 23.66%, 17.54%, 14.91%, and 11.96%. Most of the Cers structures were mainly composed of sphingadienine. A biological activity assay indicated that Cers extracted from sea red rice bran had significant antioxidant and anti-aging properties. These findings indicate that the extracted Cers show great potential for applications in the cosmetic and pharmaceutical industries.

show abstract

Optimization of ultrasonic-assisted extraction of continentalic acid from the root of Aralia continentalis by using the response surface methodology

Cited by 6 publications

References 31 publications

Optimization of Antifungal Extracts from Ficus hirta Fruits Using Response Surface Methodology and Antifungal Activity Tests

Optimization of Antifungal Extracts from Ficus hirta Fruits Using Response Surface Methodology and Antifungal Activity Tests

Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Contact Info

Product

Resources

About