Optimization of Ultrasonic—Assisted Extraction (UAE) Method Using Natural Deep Eutectic Solvent (NADES) to Increase Curcuminoid Yield from Curcuma longa L., Curcuma xanthorrhiza, and Curcuma mangga Val.

Abstract

This study aims to optimize ultrasonic-assisted natural deep eutectic solvents (NADES) based extraction from C. longa. Choline chloride-lactic acid (CCLA-H2O = 1:1, b/v) was used to investigate the impact of various process parameters such as solvent’s water content, solid loading, temperature, and extraction time. The optimal yield of 79.635 mg/g of C. longa was achieved from extraction in 20% water content NADES with a 4% solid loading in 35 °C temperature for 1 h. Peleg’s model was used to describe the kinetics of the optimized ultrasonic-assisted extraction (UAE) method, and the results were found to be compatible with experimental data. The optimum conditions obtained from C. longa extraction were then used for the extraction of C. xanthorriza and C. mangga, which give yields of 2.056 and 31.322 mg/g, respectively. Furthermore, n-hexane was utilized as an anti-solvent in the separation process of curcuminoids extract from C. longa, C. xanthorriza, and C. mangga, which gave curcuminoid recovery of 39%, 0.74%, and 27%, respectively. Solidification of curcuminoids was also carried out using the crystallization method with n-hexane and isopropanol. However, the solution of CCLA and curcuminoids formed a homogeneous mixture with isopropanol. Hence, the curcuminoids could not be solidified due to the presence of NADES in the extract solution.

Natural deep eutectic solvents (NaDES) as an emerging technology for the valorisation of natural products and agro‐food residues: a review

“…However, the extract required additional separation and chromatographical purification for a high-quality product (Rachmaniah et al, 2021). Additionally, Rosarina et al (2022) The extraction and solubilisation of curcumin can be accomplished and enhanced using NaDES (ChCl/ lactic acid) as a compatible auxiliary to surfactant-free microemulsions such as ethanol/triacetin mixtures (Huber et al, 2021). The extraction yield (90%) and solubilisation (2-fold) are outstanding despite the highly hydrophobic nature of curcumin and the very polar medium.…”

“…Additionally, Rosarina et al . (2022) optimised the curcuminoid extraction using NADES (ChCl/lactic acid, water 20%) supported with UAE. Separation and crystallisation resulted in good yields.…”

Comparison Study for the Phytochemical Constituents of two Curcuma Species by GC-MS Technique

“…21 Additionally, UAE had previously been utilised to unear th metabolites, ecological pigments, and bioactive compounds from other curcuma species. [33][34][35][36][37][38][39][40] The detection of bioactive phytoconstituents in the GC-MS examination of rhizome chloroform extracts of Curcuma caesia Roxb. and Curcuma angustifolia Roxb.…”

A Box-Behnken Design for Optimization of Ultrasound-Assisted Extraction of Sinapic Acid From Fragaria Ananassa

“…Several studies have reported successful extraction of various classes of bioactive compounds, including phenolic acids, flavonoids, alkaloids and essential oils, using ultrasound-assisted techniques [14][15][16][17][18]. For instance, UAE has been utilized to extract phenolic compounds such as resveratrol, quercetin and curcumin from different plant sources [19][20][21][22]. The enhanced extraction efficiency provided by UAE can contribute to the development of high-value natural extracts and facilitate the utilization of plant-derived bioactive compounds in various industries [3].…”