Optimization of ultrasound-assisted extraction (UAE) of phenolic compounds from Crataegus pinnatifida leaves and evaluation of antioxidant activities of extracts

Abstract

In this study, an accurate method using high pressure liquid chromatography (HPLC) has been established for the simultaneous determination of chlorogenic acid (CA), vitexin-4 00 -o-glucoside (VG), vitexin-2 00 -orhamnoside (VR), orientoside (ORT), rutin (RT), vitexin (VIT) and hyperoside (HYP) from Crataegus pinnatifida leaves. The Box-Behnken design (BBD) combined with response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction (UAE) method. Under optimized conditions (extraction temperature 41 C, extraction time 31 min, ratio of liquid/solid 15 and ethanol concentration 39%), the yields of CA, VG, VR, ORT, RT, VIT and HYP were 0.46, 0.38, 4.37, 0.033, 0.036, 0.30 and 1.19, mg g À1 , respectively. Additionally, antioxidant activities of UAE extracts in DPPH radical scavenging (DPPH), ABTS radical scavenging (ABTS) and reducing power tests were determined as 0.69 mg mL À1(IC 50 value), 0.86 mM Trolox and 0.24 mg mL À1 (IC 50 value), respectively. Compared with conventional maceration extraction (ME) and heat-refluxing extraction (HRE) methods, the UAE method showed remarkable advantages in terms of higher extraction yields and better antioxidant activities. Overall, the present results showed that UAE was an efficient and alternative method for the extraction of phenolic compounds from C. pinnatifida leaves and its extracts were potential antioxidant ingredients or additives for the food industry with potential preservation properties.

Phytochemical composition and bioactivities of hawthorn ( Crataegus spp.): review of recent research advances

“…An accurate and advanced method has been established for the simultaneous determination of chlorogenic acid, vitexin and its 4″-O-glucoside, 2″-O-rhamnoside, orientoside, rutin and hyperoside in C. pinnatifida leaves. Under optimized conditions the yields of these phytochemicals were 0.46, 0.30, 0.38, 4.37, 0.033, 0.036, and 1.19 mg/g, respectively, while DPPH • and ABTS •+ scavenging and reducing power of extract were 0.69 mg/mL (IC 50 ), 0.86 mM TE, and 0.24 mg/mL (IC 50 ), respectively (Luo et al, 2015). A simple, sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, which may serve for evaluating the pharmacokinetics, was developed and fully validated for the simultaneous determination of rutin, vitexin and its glycosides, hyperoside, shanyenoside in rat plasma after intravenous administration of hawthorn leaves flavonoids using lysionotin as an internal standard (Zhu et al, 2015).…”

Natural terpenoid glycosides with in vitro/vivo antithrombotic profiles from the leaves of Crataegus pinnatifida

“…The ethanol extract from the leaves of C. pinnatida was separated and puried by macroporous resin, polyamide, silica gel, C 18 reversed silica gel and semipreparative HPLC to afford two norditerpenoids (1-2), 13 sesquiterpenoids (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). The known compounds (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) were identied as shnyegenin B (5), 12 shnyeside B (6), 12 (3S,5R,6R,7E,9S)-megastiman-7-ene-3,5,6,9tetrol (7), 12 euodionosides D (8), 13 (6R,9R)-3-oxo-a-ionol-9-O-b-D-glucopyranoside (9), 13 (6S,7E,9R)-6,9-dihydroxy-4,7-megastiymadien-3-one-9-O-[b-D-xylopyranosy-b-D-glucopyranoside] (10), 12 linarionoside A (11), 13 linarionoside B (12), 12 linarionoside C (13), 12 Compound 1, a colorless oil, was assigned the molecular formula of C 25 H 38 O 9 as determined by the HRESIMS ion at m/z 483.2582 [M + H] + (calc. 483.2589).…”

“…6,7 An extract of hawthorn leaves, Yixintong medicinal preparations, is one of the most effective drugs available for blood-activating and stasis-dissolving. 8,9 The avonoids have been considered as the only effective component until terpenoids (monoterpenoid and sesquiterpenoid) with antithrombotic activity were obtained recently from the leaves of C. pinnatida. [10][11][12][13] As a new effective ingredient, the terpenoids exhibit potential antithrombotic activity both in vitro and in vivo.…”

“…[10][11][12][13] As a new effective ingredient, the terpenoids exhibit potential antithrombotic activity both in vitro and in vivo. 14 In continuing the search for antithrombotic agents, two novel norditerpenoids with unique carbon skeletons were isolated as the rst diterpenoids from the leaves of the title plant, together with two new sesquiterpenoids (3, 4) and 11 known ones (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). The absolute congurations of 1-4 were determined by producing of Rh 2 (OCOCF 3 ) 4 complexes or Mosher ester, and by comparison of ECD, NMR, and optical rotation (OR) data with the calculated values.…”

Ecofriendly microwave‐assisted reaction and extraction of bioactive compounds from hawthorn leaf

“…[23][24][25][26] Therefore, it is necessary to develop an effective and reliable extraction method to extract these active compounds from hawthorn leaves. At present, the shaking extraction, 27 UAE 17,28,29 and MAE 30 methods are usually used to extract bioactive compounds in hawthorn leaf, hawthorn fruit and hawthorn seed samples. Since the polyphenolic compounds in hawthorn leaf can react with alkaline solid reagents, it is meaningful to study the extraction of polyphenolic compounds from hawthorn leaf by alkaline solid reagent combined with MAE.…”