Optimization of Various Process Parameters Using Response Surface Methodology for Exopolysaccharide Production from a Novel Strain Pediococcus acidilactici KM0 (Accession Number KX671557) Isolated from Milk Cream

Abstract

Abstractediococcus acidilactici KM0 (accession number KX671557) isolated from milk cream was optimized by response surface methodology by using the Design Of Experiments (DOE) to evaluate the interactive effects of the process parameters i.e. incubation time (h), temperature (•C), pH, carbon concentration, nitrogen concentration production for the production of EPS . Maximum EPS production of 32.64 mg/ml observed after 24 h incubation at 35 •C using 1.50 % and 3% of carbon and nitrogen concentration respectively, at pH 6.5.

Optimal conditions for the production of exopolysaccharide by Pediococcus acidilactici and impact of the bacterium, its EPS and dextran on the quality of Kariesh cheese

“…Sharma et al . (2017) reported that the temperature, incubation time, pH, nitrogen and carbon concentrations are the main factors that positively affect EPS bioproduction. They found that the maximum EPS amount produced by P .…”

Screening and increase of exopolysaccharide production by rhizobial strains, stress tolerances and its efficiency with "in Vivo" peanut plants

“…Haroun et al, (2013) confirmed that Lactobacillus plantarum produced the highest amount of EPS when the pH was 6.2. Sharma et al, (2017) discovered that Pediococcus acidilactici produced maximum EPS at pH 6.0 after a 24-hour incubation period at 35 °C. Results of the inoculation amount effect have been shown by many researchers (Sivakumar et al, 2012).…”