Optimization, Production and Characterization of Polyhydroxyalkanoate (PHA) from Indigenously Isolated Novel Bacteria

Muneer, Faizan; Rasul, Ijaz; Qasim, Muhammad; Sajid, Arfaa; Nadeem, Habibullah

doi:10.1007/s10924-022-02444-y

Cited by 18 publications

(4 citation statements)

References 48 publications

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“…At the same time, the bands at 2900 to 2800 cm −1 demonstrate the presence of methyl (CH 3 ) and methylene (CH 2 ) groups. The transmittance band from 1300 –1000 cm −1 represented characteristic peaks of C=O bonding in ester group and C-O-C functional groups, which are representative of PHA functional groups [ 43 , 44 ]. Therefore, it can be concluded that the PHA obtained in this study was a copolymer of PHBV, the FTIR spectra of which were also similar to PHBV produced in other studies using different carbon sources [ 45 , 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Mahboubi

Root³

et al. 2023

Membranes

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Volatile fatty acids (VFAs) appear to be an economical carbon feedstock for the cost-effective production of polyhydroxyalkanoates (PHAs). The use of VFAs, however, could impose a drawback of substrate inhibition at high concentrations, resulting in low microbial PHA productivity in batch cultivations. In this regard, retaining high cell density using immersed membrane bioreactor (iMBR) in a (semi-) continuous process could enhance production yields. In this study, an iMBR with a flat-sheet membrane was applied for semi-continuous cultivation and recovery of Cupriavidus necator in a bench-scale bioreactor using VFAs as the sole carbon source. The cultivation was prolonged up to 128 h under an interval feed of 5 g/L VFAs at a dilution rate of 0.15 (d−1), yielding a maximum biomass and PHA production of 6.6 and 2.8 g/L, respectively. Potato liquor and apple pomace-based VFAs with a total concentration of 8.8 g/L were also successfully used in the iMBR, rendering the highest PHA content of 1.3 g/L after 128 h of cultivation. The PHAs obtained from both synthetic and real VFA effluents were affirmed to be poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with a crystallinity degree of 23.8 and 9.6%, respectively. The application of iMBR could open an opportunity for semi-continuous production of PHA, increasing the feasibility of upscaling PHA production using waste-based VFAs.

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Section: Resultsmentioning

confidence: 99%

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Mahboubi

Root³

et al. 2023

Membranes

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“…The production of polyhydroxyalkanoates is influenced by multiple factors including cultural conditions and 13 International Journal of Polymer Science nutrition. Several studies have shown that carbon and nitrogen sources as well as their concentration in fermentation media have profound impacts on PHA production [4,[50][51][52]. Thus, this study sought to optimize the fermentation conditions for Halomonas alkalicola Ext, a moderately halophilic bacterium isolated from Lake Simbi, a hypersaline lake in Kenya.…”

Section: Discussionmentioning

confidence: 99%

“…Every year, approximately 80 million metric tons of plastic wastes are released into the environment, endangering both the ecosystem and human health [2,3]. The nondegradable nature of the polymers in the synthetic plastic have led to severe soil and water pollution, thus making plastic pollution a major global issue of concern [4]. Hence, efforts aimed at developing alternative materials to synthetic polymers may help in resolving the global environmental pollution problem associated with the synthetic plastics.…”

Section: Introductionmentioning

confidence: 99%

Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

Muigano,

Anami,

Onguso

et al. 2024

International Journal of Polymer Science

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible polymers that are produced by microorganisms as storage materials under limited nutrition and excess carbon. These PHAs have been found to be ideal for replacing synthetic plastics for use in packaging and biomedical applications. In this study, an alkaliphilic and moderately halophilic bacterium Halomonas alkalicola Ext was isolated from Lake Simbi Nyaima in western Kenya and investigated for PHA production. Sudan Black B and Nile Red A staining showed that bacterium had distinct ability for accumulation of PHAs. To optimize PHA production, the bacterium was grown in submerged fermentation under varying culture conditions and different sources and concentrations of carbon and nitrogen. With one-factor-at-a-time (OFTA) approach, optimal PHA yields were obtained after 72 hours at a pH of 10.0, temperature of 35°C, and 2.5% (w/v) NaCl. The bacterium yielded the highest biomass, and PHA amounts on 2% galactose and 0.1% ammonium sulfate as sources of carbon and nitrogen, respectively. A record PHA yield of 0.071 g g-1 with a titer of 1.419±0.09 g/L was achieved from 3.397 g/L of biomass, equivalent to 41.8% PHA content. Using response surface methodology, PHA titer was increased by 1.5% to 1.44 g/L, while PHA content was improved 1.1-fold to 45.57%. Polymer analysis revealed that the extracted PHA was a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) (3−HB:3−HV=92:8) with two copolymer subunits of 3-hydroxyvaryrate (3-HB) and 3-hydroxybutyrate (3-HV). Halomonas alkalicola Ext attained efficient galactose conversion into PHBV under high salinity and alkalinity conditions.

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“…The chemical composition of the extracted powdered PHA samples was analyzed by using FTIR spectrum data. There have been some studies that used FTIR spectroscopy to characterize PHAs [24][25][26][27][28], but only a few studies indicating the influence of the composition of the nutrient media-specially supplied with different carbon sources-on the characteristics of PHAs [29,30].…”

Section: Ftir Characterization Of Phasmentioning

confidence: 99%

Polyhydroxyalkanoates (PHAs) from Endophytic Bacterial Strains as Potential Biocontrol Agents against Postharvest Diseases of Apples

et al. 2023

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Due to the increasing use and accumulation of petrochemical plastics in the environment and the rapid depletion of natural resources, microbial polyhydroxyalkanoates have great potential to replace them. This study provides new insights in the field of obtaining of polyhydroxyalkanoates (PHAs) from endophytic bacterial strains and applying them as potential biocontrol agents against postharvest diseases of apples. Two strains—Pseudomonas flavescens D5 and Bacillus aerophilus A2—accumulated PHAs in amounts ranging from 2.77 to 5.9 g L−1. The potential to use low-cost substrates such as beet molasses and soapstock for PHA accumulation was shown. The PHAs produced by the Ps. flavescens D5 strain had pronounced antagonistic activity against Penicillium expansum (antifungal property = 62.98–73.08%). The use of PHAs as biocontrol agents significantly reduced the severity of apple blue mold, especially in the preventive treatment option.

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Optimization, Production and Characterization of Polyhydroxyalkanoate (PHA) from Indigenously Isolated Novel Bacteria

Cited by 18 publications

References 48 publications

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

Polyhydroxyalkanoates (PHAs) from Endophytic Bacterial Strains as Potential Biocontrol Agents against Postharvest Diseases of Apples

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