Optimization, Purification and Characterization of Phytase from Isolated Probiotic Pediococcus acidilactici BNS5B

Abstract

Plant based diet such as vegetables, cereals, legumes and oilseeds contain 80% of total phosphorous in the form of phytic acid-cation complexes, bound phosphorous being excreted in manure due to unavailability of phytate degrader in the gastrointestinal tract of monogastric animals (Ashraf et al., 2013). The undegraded phytate leads to phosphorous deficiency in animals, elevated levels of phosphorous in soil and eutrophication of water bodies and renders phytic acid as the anti-nutritive factor by decreasing the bioavailability cations such as iron, calcium, magnesium, phosphorous, zinc, iodine, etc (Madsen, 2019;Singh et al., 2013). Most of these cations are involved in various physiological functions as their deficiency may lead to conditions such as anemia,