Optimization (Substrate and pH) and Anaerobic Fermentative Hydrogen Production by Various Industrial Wastes Isolates Utilizing Biscuit Industry Waste as Substrate

Dark fermentative biohydrogen production from rice mill wastewater

“…Further, the pretreated rice mill wastewater were analyzed for their microbial load in an aseptic environment. All the obtained 21 isolates (data not shown) were screened for bio‐H 2 production capability using Hungate technique 24 (Figure 2). The isolates RH1, RH2 were obtained after heat treatment and AARAT1 was obtained after acid treatment.…”

“…Bio‐H 2 producing bacterial strains were isolated from various pretreated samples by spread plating as described in our previous work, 24 100 μL of various pretreated rice mill wastewater samples were spread plated on sterile Thiglycollate agar (TGA) plates. Following the distinct colonial growth of the bacteria at 37°C under anaerobic conditions, the morphologically different strains were isolated and maintained on TGA slants for further usage.…”

“…Ltd) operated in batch mode was used for bio‐H 2 production. The fermenter was filled with 2.5 L of rice mill wastewater 24 and inoculated with 2% selected single bacterial strain. The fermentation was carried out in the bioreactor for 72 hours.…”

“…Each serum bottle was maintained under anaerobic conditions, incubated aseptically as reported earlier. 23,24 During fermentation, the headspace biogas was estimated utilizing the Hungate method. 25 The biogas arrangements were analyzed by GC, and the biohydrogen creation level was determined.…”

“…To obtain desirable conditions that favor biogas production, the range of influencing parameters has to be considered for the optimization study and hence, the H 2 fermentation was studied at different pH (5-8) and substrate concentrations (10%-100% of rice mill wastewater). 24,32 About 1 mL of inoculum was used in the batch experiment. All the batch experiments were conducted to generate higher bio-H 2 gas by the dark fermentation method.…”