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Photoproduction of hydrogen by Rhodopseudomonas palustris KU003 under different cultural conditions with various carbon and nitrogen sources was investigated. Hydrogen production was measured using a Gas chromatograph. Malate promoted more amounts of hydrogen production under anaerobic light conditions than anaerobic dark conditions. Cumulative hydrogen production by the organism was recorded at various time intervals. Incubation period of 120 hrs was optimum for production of hydrogen. pH 7.0 ± 0.4 was optimum for production of hydrogen. L-glutamic acid was a good nitrogen source for production of hydrogen. Growing cells produced more amount of hydrogen than resting cells. Significance of the above results in presence of existing literature is discussed.

Twenty-five fungal cultures were screened for their ability to transform the anthelmintic drug albendazole. A filamentous fungi Cunninghamella blakesleeana transformed albendazole to three metabolites in significant quantities. The transformation of albendazole was identified by HPLC. Based on the LC-MS-MS data, two metabolites were predicted to be albendazole sulfoxide and albendazole sulfone, the major mammalian metabolites reported previously. A new N-methylated metabolite of albendazole sulfoxide was also produced, where the methylation took place on the N-atom of the imidazole ring system. A temperature of 30°C, pH of 8 and high substrate concentrations produced highest transformation of albendazole. Among the various concentrations studied, 2% w/v of glucose produced highest transformation. The results reveal that the microbial model can be used to produce large quantities of mammalian metabolites.

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