Optimization, validation and comparison of various extraction techniques for the trace determination of polycyclic aromatic hydrocarbons in sewage sludges by liquid chromatography coupled to diode-array and fluorescence detection

Miège, Cécile; Dugay, J.; Hennion, M.

doi:10.1016/s0021-9673(03)00497-7

Cited by 71 publications

(27 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In recent years, there has been a growing research interest in the extraction of polycyclic aromatic hydrocarbons (PAHs) from environmental soil samples due to risks of them causing adverse effects to human health [1,2]. The conventional sample extraction methods (e.g., Soxhlet extraction and shake flask) have some drawbacks, such as long extraction time, large amount of organic solvents, labor intensive procedures and/or poor reproducibility [3].…”

Section: Introductionmentioning

confidence: 99%

Application of surfactants and microemulsions to the extraction of pyrene and phenanthrene from soil with three different extraction methods

Song

Lin

2007

Analytica Chimica Acta

View full text Add to dashboard Cite

In the present work, the use of surfactants and oil-in-water (O/W) microemulsions as alternative extractants in accelerated solvent extraction (ASE) for the extraction of polycyclic aromatic hydrocarbons (pyrene and phenanthrene) from soils was investigated. In particular, the effect of each individual component within the microemulsions, i.e., oil phase, surfactant and co-surfactatnt, and extraction conditions on the percentage recovery was systematically studied. When compared to the water and organic solvent, the important findings were that the common surfactant solutions at the concentrations above their critical micelle concentrations (CMC) were shown to enhance the percentage recovery at the lower extraction temperature. Moreover, the highest percentage recovery can be obtained using microemulsion as the extractant. The chemical component within the microemulsions and relative amounts of the oil phase appeared to play a much more significant role in ensuring high percentage recovery. Finally, an overall comparison between the percentage recoveries obtained with ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and ASE using organic solvents, surfactants and microemulsions as extractants was exhibited.

show abstract

Section: Introductionmentioning

confidence: 99%

Application of surfactants and microemulsions to the extraction of pyrene and phenanthrene from soil with three different extraction methods

Song

Lin

2007

Analytica Chimica Acta

View full text Add to dashboard Cite

show abstract

“…The highest concentration were found for pyrene, benzo[g,h,I]perylene, naphthalene Manoli and Samara (1996) and Miége et al (2003) found pyrene as the most abundant PAH in sludge. Phenanthrene was the most abundant PAH in Moreda et al (1998) and Pérez et al (2001) studies, while Lazzari et al (2000) found fluoranthene in the highest concentration, followed by pyrene and phenanthrene.…”

Section: Resultsmentioning

confidence: 99%

Evolution of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in sludge samples from conventional activated sludge wastewater treatment plants

Villar

Callejón

et al. 2008

Environmetrics

View full text Add to dashboard Cite

SUMMARYThis paper presents results on the presence and evolution of PAHs and heavy metals in a conventional activated sludge wastewater treatment plant (WWTP). The 16 PAHs recommended by the EPA were determined. On the other hand, 13 heavy metals were studied (Al, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Ti and Zn). Moreover, water content, organic and mineral matter were determined for the generic characterization of the sludges in an attempt to assess their agricultural value. The sewage sludges originated from the Guadalete WWTP site in Jerez de la Frontera (Cádiz, Spain). PAHs have been extracted by microwave energy. High performance liquid chromatography coupled with diode array and fluorescence detectors have been used (HPLC-DAD-FL). Heavy metals were analyzed by ICP-AES. The results showed that total PAHs concentration (sum of 16 EPA PAHs) varied between 6757 ng g À1 dry matter (DM) for compost and 4176 ng g À1 DM for secondary sludge. The most abundant PAH is pyrene. The highest concentration of heavy metals was also found in compost (51750 mg kg À1 DM) and lowest concentration in secondary sludge (24250 mg kg À1). In order to obtain global conclusions from all results, several chemometrical techniques of pattern recognition were applied.

show abstract

“…They concluded that DAD is useful for qualitative and quantitative analysis of PAHs in soil samples and FLD is recommended for the analysis of PAHs in water samples (Kicinski et al, 1989). HPLC-DAD/FLD has been successfully applied in the analysis of PAHs in sewage sludges (Miègea et al, 2003) and food supplements (Danyi et al, 2009). However, the PAHs were detected at a fixed wavelength of 254 nm with DAD.…”

Section: Introductionmentioning

confidence: 99%

Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors

et al. 2013

View full text Add to dashboard Cite

h i g h l i g h t sWe set up a method for determination of low levels of 16 PAHs in soil. Analyses were performed by HPLC with fluorescence and diode-array detectors. The analytical performance of the proposed method were demonstrated. a r t i c l e i n f o Risk assessment of polycyclic aromatic hydrocarbons (PAHs) contaminated soil and source apportionment require accurate analysis of the concentration of each PAH congener in the soil. However, determination of low level PAH congeners in soil is difficult because of similarity in the chemical properties of 16 PAHs and severe matrix interferences due to complex composition of soils. It is therefore imperative to develop a sensitive and accurate method for determination of low level PAHs in soil. In this work, high performance liquid chromatography equipped with fluorescence and diode-array detectors (HPLC-FLD-DAD) was used to determine the concentration of 16 PAHs in soil. The separation of the 16 PAHs was achieved by optimization of the mobile phase gradient elution program and FLD wavelength switching program. Qualitative analysis of the 16 PAHs was based on the retention time (RT) and each PAH specific spectrum obtained from DAD. In contrast, the quantitative analysis of individual PAH congeners was based on the peak areas at the specific wavelength with DAD and FLD. Under optimal conditions the detection limit was in the range 1.0-9.5 lg L À1 for 16 PAHs with DAD and 0.01-0.1 lg L À1 for 15 PAHs with FLD, and the RSD of PAHs was less than 5% with DAD and 3% with FLD. The spiked recoveries were in the range 61-96%, with the exception of NaP (<40%). The results show that HPLC-FLD-DAD can provide more accurate and reliable analysis of low level PAH congeners in soil samples.

show abstract

Optimization, validation and comparison of various extraction techniques for the trace determination of polycyclic aromatic hydrocarbons in sewage sludges by liquid chromatography coupled to diode-array and fluorescence detection

Cited by 71 publications

References 21 publications

Application of surfactants and microemulsions to the extraction of pyrene and phenanthrene from soil with three different extraction methods

Application of surfactants and microemulsions to the extraction of pyrene and phenanthrene from soil with three different extraction methods

Evolution of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in sludge samples from conventional activated sludge wastewater treatment plants

Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors

Contact Info

Product

Resources

About