Optimized amplification of the polymorphic system COL2A1

Skowasch, K.; Brinkmann, B.

doi:10.1007/bf01625175

Cited by 2 publications

(1 citation statement)

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“…For optimization of the nested approach the following PCR parameters were tested in serial experiments (Skowasch et al 1992, Brinkmann and Lung: Microscopically no typical changes (inclusion bodies) could be observed. Positive reactions were found for 1 case using ICH and 2 cases by ISH.…”

Section: Nested-pcrmentioning

confidence: 99%

CMV-DNA detection in parenchymatous organs in cases of SIDS

et al. 1995

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A nested PCR approach has been developed especially for the detection of small amounts of cytomegalovirus (CMV) DNA in autopsy samples. Lung tissue and submandibular glands in 118 cases of infant death (92 SIDS cases, 13 natural deaths due to other defined causes and 13 unnatural deaths) were investigated by this technique and compared to the results obtained by other CMV detection methods (histology, immunohistochemistry, in situ hybridization and PCR). CMV-DNA could be detected in the lung tissue in 7 cases of SIDS using nested PCR. Compared to conventional PCR (3 positive cases in lung tissue) the nested approach always gave glear results and showed less additional bands. In all cases where CMV could be detected in the lungs, positive results were also obtained in the submandibular glands. The nested PCR method proved to be a more sensitive technique than the other detection methods including PCR and hot start, and even minimal amounts of target DNA could be detected in the presence of human and bacterial background DNA.

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Section: Nested-pcrmentioning

confidence: 99%

CMV-DNA detection in parenchymatous organs in cases of SIDS

et al. 1995

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Human Identity Testing with PCR-based Systems

Hohoff

Brinkmann

1999

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Large numbers of repetitive stretches of DNA are present within the human genome that are associated with human individuality due to their polymorphic character. Approximately one-third of these repeat sequences is arranged as microsatellites or short tandem repeats (STRs) whose valuable application as state-of-the-art technique in human identity testing will be briefly summarized in this review. Prerequisites for successful DNA typing using STRs amplified by polymerase chain reaction (PCR) are outlined and particular attention is paid to the molecular structure of STRs from autosomes as well as from the Y chromosome. A comprehensive overview about current and emerging methods of STR analysis is given as well.

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Optimized amplification of the polymorphic system COL2A1

Cited by 2 publications

References 10 publications

CMV-DNA detection in parenchymatous organs in cases of SIDS

CMV-DNA detection in parenchymatous organs in cases of SIDS

Human Identity Testing with PCR-based Systems

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