Optimized dispersion of ZnO nanoparticles and antimicrobial activity against foodborne pathogens and spoilage microorganisms

Espitia, Paula Judith Pérez; Soares, Nilda de Fátima Ferreira; Teófilo, Reinaldo F.; Vitor, Débora Monique; Coimbra, Jane Sélia dos Reis; Andrade, Nélio José de; Sousa, Frederico B. De; Sinisterra, Rubén D.; Medeiros, Éber Antônio Alves

doi:10.1007/s11051-012-1324-4

Cited by 26 publications

(7 citation statements)

References 45 publications

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“…Furthermore, the obtained results indicated that ZnO-NP have greater antibacterial activity against APC with high reduction percent (99.5%) in the treated group with ZnO-NP 10 mM than other groups as the antibacterial activity increased with increasing concentration. These results were nearly similar to those reported by Raghupathi et al (2011), Espitia et al (2013) and Rezk (2018). Antibacterial properties of ZnO-NPs depends on the physicochemical properties of NPs including their size, charge, surface morphology, and crystal structure, which are significant elements that regulate the actions of NPs on bacterial cells.…”

Section: Resultssupporting

confidence: 92%

Antibacterial Effect of Zinc Oxide Nanoparticles in Fresh Meat

saafan¹,

Amin

Eleiwa³

et al. 2019

Benha Veterinary Medical Journal

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Practical application of suspension with different concentrations (5mM, 8mM, and 10mM) were investigated to evaluate its antibacterial effect in fresh meat. A total number of 12 samples of fresh meat were collected from different abattoirs (150 gm. of each) in Gharbia Governorate, Egypt, under complete aseptic conditions and transferred without undue delay to the Lab to evaluate the efficacy of ZnO NPs as antibacterial agents in fresh meat. The obtained results indicated that ZnO-NPs had a significant inhibitory effect on the growth of APC and Staphylococcus aureus during 6 days of refrigerator storage at 4 0C. At zero day, the mean values of APC in the control group was 3.27x107±6.05x106cfu/g, while after treatment with ZnO-NPs 5mM, 8mM and 10mM the mean values were decreased to 1.67x107±9.11x106, 1.55x107±9.73x106, and 9.41x106±7.67x105cfu/g, respectively. By the 3rd day of refrigeration storage the control group showed complete spoilage. While the mean values of Staphylococcus aureus at zero day in control group was 3.09x107±2.33x107cfu/g, while after treatment with ZnO-NPs 5mM, 8mM and 10mM the mean values were highly decreased to 1.40x107±9.56x106, 1.79x106±4.70x105, and 8.05x105±9.51x105cfu/g, respectively. Accurately, ZnO-NPs with concentration 10mM showed the highest reduction percentage 99.5% and 99.85% to APC and Staphylococcal aureus, respectively, from 3.27x107±6.05x106to 1.64x105±2.47x104 and 3.09x107±2.33x107 to 4.61x104±4.44x104 respectively, compared to other concentrations (5mM and 8mM). Thus the best sensory quality was obtained by ZnO-NP 10mM indicating to the fact that the antibacterial activity of ZnO-NPs is concentration dependent. Zinc oxide nanoparticles Antibacterial activity Aerobic plate countStaphylococcus aureus.

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Section: Resultssupporting

confidence: 92%

Antibacterial Effect of Zinc Oxide Nanoparticles in Fresh Meat

saafan¹,

Amin

Eleiwa³

et al. 2019

Benha Veterinary Medical Journal

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“…Furthermore, ZnO particles have the potential to become a next generation biocide or disinfecting agent, due to their stability and lower probability of developing resistances in comparison to their organic-based counterparts [9][10][11]. The antimicrobial properties of ZnO have been suggested to serve as a means of packaged food preservation [12][13][14]. Such intentive intake makes ZnO toxicity an issue of the highest importance.…”

Section: Introductionmentioning

confidence: 99%

Morphological impact of zinc oxide particles on the antibacterial activity and human epithelia toxicity

Čepin

Hribar

Caserman

et al. 2015

Materials Science and Engineering: C

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“…To test the antimicrobial activity of LFcinB, FBS and antibiotic-free media from the transfected bMESCs were analyzed for antibacterial activity. The antimicrobial activity was determined by agar-well diffusion method [ 51 , 52 ]. S. aureus and E. coli were grown in Brain Heart Infusion (BHI) broth and incubated for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

A PiggyBac mediated approach for lactoferricin gene transfer in bovine mammary epithelial stem cells for management of bovine mastitis

et al. 2017

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The antibacterial and anti-inflammatory properties of lactoferricin have been ascribed to its ability to sequester essential iron. The objective of the study was to clone bovine lactoferricin (LFcinB) gene into PiggyBac Transposon vector, expression study in the bovine mammary epithelial stem cells (bMESCs) and also to determine the antimicrobial property of recombinant LFcinB against bovine mastitis-causing organisms. The PiggyBac-LFcinB was transfected into bMESCs by electroporation and a three fold of LFcinB secretion was observed in the transfected bMESCs medium by ELISA assay. Furthermore, the assessment of antimicrobial activity against mastitis causing pathogens Staphylococcus aureus and Escherichia coli demonstrated convincing evidence to prove strong antibacterial activity of LFcinB with 14.0±1.0 mm and 18.0±1.5 mm zone of inhibition against both organisms, respectively. The present study provides the convincing evidence to suggest the potential of PiggyBac transposon system to transfer antibacterial peptide into bMESCs or cow mammary gland and also pave the way to use bovine mammary gland as the bioreactors. Simultaneously, it also suggest toward commercial utilization of LFcinB bioreactor system in pharmaceutical industry.

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Optimized dispersion of ZnO nanoparticles and antimicrobial activity against foodborne pathogens and spoilage microorganisms

Cited by 26 publications

References 45 publications

Antibacterial Effect of Zinc Oxide Nanoparticles in Fresh Meat

Antibacterial Effect of Zinc Oxide Nanoparticles in Fresh Meat

Morphological impact of zinc oxide particles on the antibacterial activity and human epithelia toxicity

A PiggyBac mediated approach for lactoferricin gene transfer in bovine mammary epithelial stem cells for management of bovine mastitis

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