Optimized dual assay for the transgenes selection and screening in CHO cell line development for recombinant protein production

Beketova, Elena; Ibneeva, Liliia R; Abdulina, Yulia A; Dergousova, E. A.; Филатов, Владимир Владимирович; Kozlovsky, Stanislav V.; Shilov, E. S.; Datskevich, Petr N.; Rozov, F. N.

doi:10.1007/s10529-019-02711-4

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…The protein processing abilities and the N-and O-glycosylation profiles also differed significantly across the host cell lines, suggested the necessity of choosing host cells in a rational manner for cellline development on the basis of the recombinant protein being produced (Lakshmanan et al, 2019). A simple technique was also developed to screen multiple CHO cell clones for cell growth rate and protein production (Beketova et al, 2019). Additionally, a multi-omics study was carried out on the impact of cysteine feed level on cell viability and IgG 1 mAb production in 5 l bioreactors using CHO cells so as to obtain an in-depth understanding of the CHO cell biology (Ali et al, 2019).…”

Section: Mammalian Cellsmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

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Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.

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Section: Mammalian Cellsmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

View full text Add to dashboard Cite

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“…This is quite obvious when clonally derived cell populations from a single transfection are compared against each other. These phenotypic differences have impacts on growth-related characteristics, cellspecific productivity and the quality of the final product (e.g., glycosylation patterns) [6,7]. Screening and profiling methods have been introduced (amongst others by [5,7]) to assess cell growth rate, cell-specific productivity and glycosylation patterns, along with further quality attributes of the produced recombinant proteins.…”

Section: Introductionmentioning

confidence: 99%

Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study

Rodríguez

Morerod²,

Pörtner

et al. 2021

Processes

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For the manufacturing of complex biopharmaceuticals using bioreactors with cultivated mammalian cells, high product concentration is an important objective. The phenotype of the cells in a reactor plays an important role. Are clonal cell populations showing high cell-specific growth rates more favorable than cell lines with higher cell-specific productivities or vice versa? Five clonal Chinese hamster ovary cell populations were analyzed based on the data of a 3-month-stability study. We adapted a mechanistic cell culture model to the experimental data of one such clonally derived cell population. Uncertainties and prior knowledge concerning model parameters were considered using Bayesian parameter estimations. This model was used then to define an inoculum train protocol. Based on this, we subsequently simulated the impacts of differences in growth rates (±10%) and production rates (±10% and ±50%) on the overall cultivation time, including making the inoculum train cultures; the final production phase, the volumetric titer in that bioreactor and the ratio of both, defined as overall process productivity. We showed thus unequivocally that growth rates have a higher impact (up to three times) on overall process productivity and for product output per year, whereas cells with higher productivity can potentially generate higher product concentrations in the production vessel.

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Directed evolution approach to enhance efficiency and speed of outgrowth during single cell subcloning of Chinese Hamster Ovary cells

Weinguny

Klanert

Eisenhut

et al. 2020

Computational and Structural Biotechnology Journal

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Optimized dual assay for the transgenes selection and screening in CHO cell line development for recombinant protein production

Cited by 3 publications

References 17 publications

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection—A Simulation Study

Directed evolution approach to enhance efficiency and speed of outgrowth during single cell subcloning of Chinese Hamster Ovary cells

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