Optimized extraction of anti-cancer compound – cucurbitacin I and LC–MS identification of major metabolites from wild Bottle gourd (Lagenaria siceraria (Molina) Standl.)

Attar, U. A.; Ghane, Savaliram G.

doi:10.1016/j.sajb.2018.09.006

South African Journal of Botany

2018

DOI: 10.1016/j.sajb.2018.09.006

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Optimized extraction of anti-cancer compound – cucurbitacin I and LC–MS identification of major metabolites from wild Bottle gourd (Lagenaria siceraria (Molina) Standl.)

U. A. Attar

Savaliram G. Ghane

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Cited by 36 publications

(34 citation statements)

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“…Such antioxidant potential may evaluated by several antioxidant assays (DPPH radical scavenging assay, ABTS radical cation scavenging and phosphomolybdate assay). Members of the family Cucurbitaceae have proved to be promising antioxidants as reported by earlier workers 2,3,17,22 . In addition, various methods have been employed for the analysis of cucurbitacins using TLC, FTIR, HPLC, LC-MS 17,23 .…”

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confidence: 68%

“…Members of the family Cucurbitaceae have proved to be promising antioxidants as reported by earlier workers 2,3,17,22 . In addition, various methods have been employed for the analysis of cucurbitacins using TLC, FTIR, HPLC, LC-MS 17,23 . Hence, the goals of the current study were to optimize extraction of CUI, CUB and CUI + B using HPLC and RSM as a suitable statistical tool.…”

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confidence: 68%

“…These enable us in obtaining a better quality and high efficiency of targeted yield from the plant. Hence, one has to optimize the recovery of the metabolites using extraction methods 16,17 .…”

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confidence: 99%

“…Response surface methodology (RSM) is a statistical tool usually used in optimization of bioactive compound extraction 17,18 . In RSM, optimized responses, i.e.…”

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confidence: 99%

“…In RSM, optimized responses, i.e. output variables are controlled by several independent (input) variables and found helpful to cut the number of experimental trials needed to test multiple parameters and their interactions 17,19,20 . Therefore, in present work, RSM with Box-Behnken Design (BBD) was used as a statistical tool for the optimization of cucurbitacin extraction.…”

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confidence: 99%

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Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Diplocyclos palmatus (L.) C. Jeffrey is an important medicinal plant used in several reproductive medicines. It serves as a wide source of tetracyclic triterpens called cucurbitacins. Response surface methodology (RSM) with Box-Behnken design (BBD) was studied to optimize the production of cucurbitacins. RSM put forth the ideal conditions such as 1:30 SS ratio (g/mL), 80 rpm (mixing extraction speed), 150 µm mean particle size, 30 min extraction time and 50 °C using chloroform in continuous shaking extraction (CSE) and showed the highest cucurbitacin I (CUI) content (2.345 ± 0.1686 mg/g DW). Similarly, the highest yield of cucurbitacin B (CUB) (1.584 ± 0.15 mg/g DW) was recorded at ideal conditions (1:40 g/mL SS ratio and 60 min time and others similar to CUI). Among the tested extraction methods, the highest CUI, CUB, and CUI + B yield (1.437 ± 0.03, 0.782 ± 0.10, 2.17 ± 0.35 mg/g DW, respectively) as well as promising DPPH radical scavenging activity (25.06 ± 0.1 µgAAE/g DW) were recorded from the SBAE (steam bath assisted extraction). In addition, MAE and UAE revealed the highest inhibition of α-amylase (68.68%) and α-glucosidase (56.27%) enzymes, respectively. Fruit extracts showed potent anticancer activity against breast (MCF-7) and colon (HT-29) cancer cell lines (LC 50 -44.27 and 46.88 µg/mL, respectively). Our study proved that SS ratio, particle size and temperature were the most positively influencing variables and served to be the most efficient for the highest recovery of CUI and CUB. Based on the present study, the fruits of D. palmatus were revealed as a potent antioxidant, anti-diabetic and anticancer bio-resource that could be explored further to develop novel drug to manage diabetes, cancer and oxidative stress related disorders.The plants from the family Cucurbitaceae are commonly called as melons, squashes, and gourds which are traditionally used in the human diet. It comprises 122 genera and 940 species of which 31 genera and 94 species are found in India 1 . Diplocyclos palmatus (L.) C. Jeffrey is a slender-stemmed tendril climber commonly called as Shivlingi. Traditionally, this plant has been used in the folk medicine and possesses several activities such as gynaecological, anti-asthmatic, anti-convulsant, anti-venom, anti-inflammatory, androgenic and antioxidant 2-4 . The family Cucurbitaceae has been recognized as a rich source of bitter compounds, called cucurbitacins 5 . The cucurbitacins are highly unsaturated triterpenes containing many keto-, hydroxy-and acetoxy-groups. They are the major active components in traditional medicine, herbal remedies and Pharmacopoeia to treat various health issues such as hepatoprotective, anti-fertility, diabroticites cardiovascular and anti-inflammatory activities 6 . In a recent study, cucurbitacin I (CUI) has been identified as a strong inhibitor of the JAK2/STAT3 signaling pathway (a common oncogenic signaling pathway), which is constitutively activated in many types of cancer; hence, it is considered as a milestone in cancer therapy 7 . Similarly...

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confidence: 68%

mentioning

confidence: 68%

mentioning

confidence: 99%

“…Response surface methodology (RSM) is a statistical tool usually used in optimization of bioactive compound extraction 17,18 . In RSM, optimized responses, i.e.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Bioactive Aqueous Fraction of Edible Trigonella foenum‐graecum, Piper betel, and Lagenaria siceraria Homogenate Downregulate the Inflammatory Mediators: In‐silico and In‐vitro Appraisal

Karmakar,

Ikbal,

Bhardwaj

et al. 2024

ChemistrySelect

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Traditional nutraceutical therapy is a promising approach in the management of inflammatory disorders. Fenugreek, bottle gourd, and betel leaf are rich sources of polyphenolic secondary metabolites. They are abundantly found in the Indian subcontinent for the preparation of healthy traditional functional food. Traditional evidence recommended that these herbs could control inflammation. The study was conducted with those herbs to validate their protective role against inflammatory mediators of arthritis and asthma through in‐silico and in‐vitro models. Phytochemical tests revealed the presence of flavonoids, amino acids, polyphenols, and saponins. Molecular docking was performed against six potential inflammatory bio‐marker proteins, like, LOX‐5, 15‐LOX, PLA2, IL‐6, TNFR1 & TNF‐α with the phyto‐marker compounds of test herbs and found satisfactory binding scores against inflammatory bio‐markers. Extended studies with Fenugreek and betel leaf aqueous fractions showcased 87 % and 91 % inhibition of protein denaturation at 200 μg/ml. Bottle‐gourd and betel‐leaf demonstrated 96 % and 98 % inhibition against 15‐Lipoxygenase at 100 μg/ml. TNF‐α and IL‐6 were dropped significantly by 49 % and 36 % upon treatment of betel leaf at 200 μg/ml from the LPS‐stimulated PBMC cultured cells. Findings may recommend the development of commercial anti‐inflammatory polyherbal phytopharmaceuticals for controlling polyarthritis and inflammatory complications subject to preclinical in‐vivo and clinical investigation.

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RP-HPLC analysis of phenolic compounds, quantitative assessment of phytochemicals, antioxidant, analgesic, and anti-inflammatory potential of Cucumis melo var. inodorus fruit growing in Algeria

Barghout,

Djidel,

Bouaziz

et al. 2024

Food Measure

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Optimized extraction of anti-cancer compound – cucurbitacin I and LC–MS identification of major metabolites from wild Bottle gourd (Lagenaria siceraria (Molina) Standl.)

Cited by 36 publications

References 28 publications

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Bioactive Aqueous Fraction of Edible Trigonella foenum‐graecum, Piper betel, and Lagenaria siceraria Homogenate Downregulate the Inflammatory Mediators: In‐silico and In‐vitro Appraisal

RP-HPLC analysis of phenolic compounds, quantitative assessment of phytochemicals, antioxidant, analgesic, and anti-inflammatory potential of Cucumis melo var. inodorus fruit growing in Algeria

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