Optimized formulation of multivesicular liposomes loaded with oleanolic acid enhanced anticancer effect in vitro

Abstract

Invasion and metastasis are the main causes leading to the death of patients with hepatocellular carcinoma (HCC). Multivesicular liposomes loaded with oleanolic acid (OA-MVLs) have been well demonstrated to suppress survival, growth and angiogenesis of HCC cells. Emerging evidence demonstrates that OA was able to suppress the invasion of HCC cells by down-regulating myocyte enhancer factor-2. We hypothesized that the optimized OA-MVLs could inhibit the migration and invasion of HCC cells. In this study, we utilized central composite design and response surface methodology to assess the influence of some parameters on particle size and encapsulation efficiency and obtain the optimized formulation of OA-MVLs. Subsequently, the human HCC cell lines SMMC-7721 and HepG2 were treated with different doses of OA-MVLs and OA, respectively. Cellular survival, adhesion, migration and invasion in vitro were evaluated. We found that the optimized OA-MVLs significantly decreased the ability of HCC cells to adhere, migrate and invade in vitro. Furthermore, OA-MVLs significantly inhibited the survival of HCC cells at 160 µmol/L but showed no obvious inhibition effect on the cell vitality of normal liver cells. Our findings indicate that OA-MVLs did inhibit the cell survival, adhesion, invasion and metastasis of HCC cells in vitro. Although the involved mechanisms are still unclear, our findings can contribute to a better development of a preventive and therapeutic strategy for human HCC.

Multivesicular Liposomes for the Sustained Release of Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Peanuts: Design, Characterization, and In Vitro Evaluation

“…The zeta potential values were −67.29 ± 3.55 mV. Zeta potentials greater than 30 mV or less than −30 mV can stabilize these double emulsions [21].…”

Bone-targeting drug delivery system of biomineral-binding liposomes loaded with icariin enhances the treatment for osteoporosis

“…Liposomes were prepared by a combined method of a thin-film dispersion and a mechanical extrusion, including a main component of soybean lecithin (A), cholesterol (B), and PPi-TEG-Chol (C). The component ratio of BBL and NBL was optimized according to our previous report by central composite design that is widely used in designation of pharmaceutical dosage forms [39]. As shown in Table 1, to prepare BBL [A/B/C: 70/20/10 (molar ratios)] and NBL [A/B/C: 70/30/0 (molar ratios)], mixture of lipids and icariin was dissolved in 10 mL solvent of chloroform/methanol (1:1, v/v) and placed into a round bottom flask.…”

Injectable Lipid-Based Depot Formulations: Where Do We Stand?

“…Although mostly hydrophilic drugs were encapsulated very efficiently owing to the large aqueous compartments in the MVLs, various attempts were made to include hydrophobic drugs into this system. Luo et al encapsulated oleanolic acid, a pentacyclic triterpenoid, into the bilayer of the MVLs and in vitro and in vivo showed a prolonged release and plasma concentrations superior to free oleanolic acid, respectively [ 68 , 69 ]. A different approach to include a hydrophobic agent into the MVL was pursued by Vafaei and coworkers using cyclodextrins [ 70 ].…”