Optimized GC-MS Method To Simultaneously Quantify Acetylated Aldose, Ketose, and Alditol for Plant Tissues Based on Derivatization in a Methyl Sulfoxide/1-Methylimidazole System

Li, Ké; Liu, Shichang; Tan, Yupeng; Chen, Nan; Tian, Xiaoming; Liu, Qi; Powell, William A.; Jiang, Xiangning; Gai, Ying

doi:10.1021/jf3053862

Cited by 30 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, Malagon et al and Zhang et al detected rhamnitol in human urine using GC/MS (Malagon et al, 2006) and LC/MS with HILIC (Zhang, Creek, Barrett, Blackburn, & Watson, 2012). Rhamnitol was detected in the leaves of Arabidopsis thaliana, corn grains (Zea maize), and the fruit of cucumber (Cucumis sativus) by GC/MS; however, it was not found in Fuji apples, in contrast to our results (Li et al, 2013;Tan et al, 2010). In the only study of fruits, Yun et al identified rhamnitol in Satsuma mandarin (Citrus unshiu Marcovitch) (Yun et al, 2013).…”

Section: Apple Characterisation By Targeted Analysis With Annotated Mcontrasting

confidence: 99%

A NMR-based, non-targeted multistep metabolic profiling revealed l-rhamnitol as a metabolite that characterised apples from different geographic origins

et al. 2015

View full text Add to dashboard Cite

Section: Apple Characterisation By Targeted Analysis With Annotated Mcontrasting

confidence: 99%

A NMR-based, non-targeted multistep metabolic profiling revealed l-rhamnitol as a metabolite that characterised apples from different geographic origins

et al. 2015

View full text Add to dashboard Cite

“…Glucose and fructose were extracted from Arabidopsis plant leaves and quantified using a new approach as described 36 . Fifty milligrams of fully expanded rosette leaves from 3-week-old Arabidopsis plants were harvested at the end of night.…”

Section: Methodsmentioning

confidence: 99%

AtSWEET4, a hexose facilitator, mediates sugar transport to axial sinks and affects plant development

Liu

Zhang

Yang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Plants transport photoassimilates from source organs to sink tissues through the phloem translocation pathway. In the transport phloem, sugars that escape from the sieve tubes are released into the apoplasmic space between the sieve element/companion cell complex (SE/CC) and phloem parenchyma cells (PPCs) during the process of long-distance transport. The competition for sugar acquisition between SE/CC and adjoining PPCs is mediated by plasma membrane translocators. YFP-tagged AtSWEET4 protein is localized in the plasma membrane, and PromoterAtSWEET4-GUS analysis showed that AtSWEET4 is expressed in the stele of roots and veins of leaves and flowers. Overexpression of AtSWEET4 in Arabidopsis increases plant size and accumulates more glucose and fructose. By contrast, knock-down of AtSWEET4 by RNA-interference leads to small plant size, reduction in glucose and fructose contents, chlorosis in the leaf vein network, and reduction in chlorophyll content in leaves. Yeast assays demonstrated that AtSWEET4 is able to complement both fructose and glucose transport deficiency. Transgenic plants of AtSWEET4 overexpression exhibit higher freezing tolerance and support more growth of bacterium Pseudomonas syringae pv. phaseolicola NPS3121. We conclude that AtSWEET4 plays an important role in mediating sugar transport in axial tissues during plant growth and development.

show abstract

“…Derivatization of RA samples was done following the previously described method [26]. This method was concurrently used to generate the GC-MS chromatogram of the 22 monosaccharide, disaccharide, and alditol standard substances.…”

Section: Methodsmentioning

confidence: 99%

Potential Quality Evaluation Method for Radix Astragali Based on Sweetness Indicators

Gao

Qin

et al. 2015

Molecules

Self Cite

View full text Add to dashboard Cite

Sweetness is a traditional sensory indicator used to evaluate the quality of the popular Chinese herb Radix Astragali (RA). RA roots with strong sweetness are considered to be of good quality. However, neither a thorough analysis of the component(s) contributing to RA sweetness, nor a scientific investigation of the reliability of this indicator has been conducted to date. In this study, seven kinds of sweetness components were identified in RA and a quality evaluation method based on these components was established and used to characterize the quality of 48 RA samples. The sweetness evaluation method of RA was first built based on the sweetness components, and a comprehensive evaluation index commonly used in quality control of RA was also derived, which was based on the contents of four indicators (astragaloside IV, calycosin glucoside, polysaccharides and extracts). After evaluating the correlation of these indexes the results showed that the level of sweetness exhibited a strong positive correlation with the proposed comprehensive index. Our results indicate that sweetness is one of the most important quality attributes of RA and thus provide a scientific basis for the utility of the sweetness indicator in quality assessment of this Chinese herb.

show abstract

Optimized GC-MS Method To Simultaneously Quantify Acetylated Aldose, Ketose, and Alditol for Plant Tissues Based on Derivatization in a Methyl Sulfoxide/1-Methylimidazole System

Cited by 30 publications

References 21 publications

A NMR-based, non-targeted multistep metabolic profiling revealed l-rhamnitol as a metabolite that characterised apples from different geographic origins

A NMR-based, non-targeted multistep metabolic profiling revealed l-rhamnitol as a metabolite that characterised apples from different geographic origins

AtSWEET4, a hexose facilitator, mediates sugar transport to axial sinks and affects plant development

Potential Quality Evaluation Method for Radix Astragali Based on Sweetness Indicators

Contact Info

Product

Resources

About