Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols

Optimized pressurized hot water extraction, HPLC/LC‐MS characterization, and bioactivity of Tetrapleura tetraptera L. dry fruit polyphenols

“…The MBC of TTP recovered using PHWE at which no visible growth of bacteria was observed were 2.0 mg L −1 for both S. aureus and B. subtilis and 4.0 mg L −1 for E. coli (Table 4). In an earlier study done by Dzah (2022) using ultrasound assisted extraction of TTP, the MBC observed for S. aureus and B. subtilis were similar to those in this study. The notable difference was the significant reduction in colonies of bacteria in this study compared to the earlier (Dzah, 2022).…”

“…In an earlier study done by Dzah (2022) using ultrasound assisted extraction of TTP, the MBC observed for S. aureus and B. subtilis were similar to those in this study. The notable difference was the significant reduction in colonies of bacteria in this study compared to the earlier (Dzah, 2022). For example, for E. coli , a 0.5 mg L −1 TTP treatment resulted in reduction of colony forming units (CFU) from 5.62 × 10 7 to 8.7 × 10 6 in the earlier study compared to a reduction from 6.28 × 10 7 to 6.0 × 10 5 CFU in this study.…”

“…Other studies have also examined the chemical composition of TT (Akin‐Idowu et al., 2011; Nwoba, 2015). A recent study conducted on ultrasound‐recovered TT polyphenols (TTP) showed that TTP extract contained 24 phenolic acids and flavonoids, which exhibited significant cytotoxic effects in human liver cancer cells (HepG2), cellular antioxidant potential in HepG2, and reactive oxygen species (ROS) scavenging activity according to DPPH and ABTS assays (Dzah, 2022). TTP extract also showed significant antibacterial properties against some foodborne pathogens (Achi, 2010; Ebana et al., 2016; Ekwenye & Okorie, 2010).…”

Sonoprocessing improves phenolics profile, antioxidant capacity, structure, and product qualities of purple corn pericarp extract

“…Nevertheless, as the extraction time increases beyond the optimal time, CT begins to decrease, possibly due to CT's transformation into anthocyanins. A comparable trend has been stated by Lv et al [60] , Dzah [24] , and Thilakarathna and Rupasinghe [61] on CT extraction using ultrasound. In terms of extraction time and TPC, it was observed that the influence was significant ( p < 0.05), as shown in Table 3 , and a quadratic effect was observed, as seen in the RS and perturbation plot ( Fig.…”

“…Six potential parameters for phenolic compounds’ extraction were evaluated, as discussed in 2.3.2, which were centered on preliminary experiments and works of literature [7] , [8] , [22] , [23] , [24] . The experiment consisted of 13 runs with different parameter permutations, which were graded at low (-), high (+), and middle (0) levels.…”