Optimizing factors affecting adventitious shoot regeneration, in vitro flowering and fruiting of Withania somnifera (L.) Dunal

Sivanesan, Iyyakkannu; Park, Se Won

doi:10.1016/j.indcrop.2015.05.014

Cited by 29 publications

(12 citation statements)

References 34 publications

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“…In this study, including 4% and 5% sucrose in the MS nutrient medium promoted flowering in C. roseus (Table 4). Similar results have been reported for Ceropegia rollae [58], Scrophularia takesimensis [40], and Withania somnifera [57]. The in vitro flowering procedure established in this study can be utilized in bioactive compounds, mainly alkaloid [27] production, and in vitro breeding of C. roseus.…”

Section: Discussionsupporting

confidence: 84%

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Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

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This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

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Section: Discussionsupporting

confidence: 84%

“…The in vitro flower induction depends on culture environment, PGRs, media composition, and sucrose level [56]. The in vitro flowering of C. roseus is also affected by sucrose (Table 4), which promotes in vitro flowering in many plants [40,57,58]. Recently, C. roseus in vitro flowering has been achieved by using silver nitrate [27].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

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“…Some plants may respond positively to the addition of photosynthetic photon flux, particularly under mixotrophic/photoautotrophic conditions (CO 2 -rich and low sugar level). Temperature also affects different biological processes such as photosynthesis and respiration [ 131 ]. Although the growth chamber temperature commonly ranges from 20 to 27 °C, the optimal temperature can vary based on the genotype.…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

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Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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“…The success of in vitro plant culture depends on several factors, such as genotype, the physiological status of the donor plants, the type of explants, surface disinfection methods, the culture medium, plant growth regulators, the size of the culture vessels, spectral quality, light intensity, photoperiod and temperature. 2 The composition of the culture medium strongly inuences the morphogenetic potential of the explants. The medium generally consists of macro-and micronutrients, amino acids, organic supplements, vitamins, carbon sources, plant growth regulators, and solidifying agents.…”

Section: Introductionmentioning

confidence: 99%