Optimizing methods to characterize caffeic, ferulic, and chlorogenic acids in Salicornia sinus-persica and Salicornia bigelovii extracts by tandem mass spectrometry (LC-MS/MS)

Abstract

Three types of phenolic acids (caffeic, ferulic, and chlorogenic acid) were identified and quantified in two halophytic plants (Salicornia bigelovii and Salicornia sinus-persica). Analysis was performed using a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system via the standard addition method. The matrix effect (ME), recovery efficiency (RE), and process efficiency (PE) parameters were evaluated for S. bigelovii extracts, which revealed a strong ME on the ionization of the three acids. The matrix had little to no effect on the extraction step for all samples. The quantitation results revealed that S. bigelovii was more abundant in the acids analyzed (total of 319.2 µg/g dry water extract) when compared to S. sinus-persica (117.2 µg/g dry water extract), and chlorogenic acid was the dominant component. The extractives content was high in both plants, representing over 35% of the dry plant weight. All the extracts exhibited antioxidant activity toward both DPPH and ABTS radicals at levels comparable to other medicinal plants.

Antioxidant, Antihypertensive, Hypoglycaemic and Nootropic Activity of a Polyphenolic Extract from the Halophyte Ice Plant (Mesembryanthemum crystallinum)

“…However, it is important to note that these extracts were obtained using 80% methanol, which significantly affects the final antioxidant properties. The antioxidant capacity determined by TEAC showed lower values than those reported by Cybulska et al [ 27 ] in polyphenol extracts of Salicornia sinus-persica and Salicornia bigelovii (1227 and 4795 μmol/g, respectively).…”

Overview of the Polyphenols in Salicornia: From Recovery to Health-Promoting Effect

“…Significant content (up to 57.52% of total compounds) of flavonoids and flavonoid glycosides have been revealed in S. bigelovii and S. europaea extracts. Other studies reported in the literature have isolated rutin hydrate (10.05 mg/g dw) and catechin hydrate (1.17 mg/g dw) from S. europea [24,39,56].…”

Bioactive Substances of Potato Juice Reveal Synergy in Cytotoxic Activity against Cancer Cells of Digestive System Studied In Vitro

“…The same LC-MS/MS system, as for α -solanine and α -chaconine determination, was used for the determination of phenolic acids (PAs), including caffeic (CaA), chlorogenic (ChA), and ferulic (FA) acids, according to the method described by Cybulska et al [ 39 ]. Chromatographic separation was achieved on the analytical column Luna 3 µm C18 (150 mm × 2.0 mm I.D., Phenomenex Inc., Torrance, CA, USA) using 5 mM ammonium acetate in water (A) and methanol (B) as mobile phase in a gradient mode of elution: 0 min 50% B, 2.5 min 50% B, 3 min 100% B, and 3.5 min 100% B.…”