Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification

Qiu, Jingwen; Wang, Qing; Shen, Fei; Yang, Gang; Zhang, Yanzong; Deng, Shihuai; Zhang, Jing; Zeng, Yongmei; Song, Chun

doi:10.1007/s12010-016-2273-7

Cited by 22 publications

(13 citation statements)

References 31 publications

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“…The results demonstrated that more lignin (98.0%) was removed. Higher cellulose–glucose conversion (95.0%) was achieved with lower cellulase consumption (6.9 FPU/g glucan) using the pretreatment method in this research compared with the previous reports (about 77–94%) with 20 FPU/g glucan [ 39 , 40 ]. Probably, the lignin removal could result in great increase of the enzymatic hydrolysis efficiency.…”

Section: Resultscontrasting

confidence: 39%

Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method

Wang

et al. 2017

Biotechnol Biofuels

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BackgroundIsoprene as the feedstock can be used to produce renewable energy fuels, providing an alternative to replace the rapidly depleting fossil fuels. However, traditional method for isoprene production could not meet the demands for low-energy consumption and environment-friendliness. Moreover, most of the previous studies focused on biofuel production out of lignocellulosic materials such as wood, rice straw, corn cob, while few studies concentrated on biofuel production using peanut hull (PH). As is known, China is the largest peanut producer in the globe with an extremely considerable amount of PH to be produced each year. Therefore, a novel, renewable, and environment-friendly pretreatment strategy to increase the enzymatic hydrolysis efficiency of cellulose and reduce the inhibitors generation was developed to convert PH into isoprene.ResultsThe optimal pretreatment conditions were 100 °C, 60 min, 10% (w/v) solid loading with a 2:8 volume ratio of phosphoric acid and of hydrogen peroxide. In comparison with the raw PH, the hemicellulose and lignin were reduced to 85.0 and 98.0%, respectively. The cellulose–glucose conversion of pretreated PH reached up to 95.0% in contrast to that of the raw PH (19.1%). Only three kinds of inhibitors including formic acid, levulinic acid, and a little furfural were formed during the pretreatment process, whose concentrations were too low to inhibit the isoprene yield for Escherichia coli fermentation. Moreover, compared with the isoprene yield of pure glucose fermentation (298 ± 9 mg/L), 249 ± 6.7 and 294 ± 8.3 mg/L of isoprene were produced using the pretreated PH as the carbon source by the engineered strain via separate hydrolysis and fermentation and simultaneous saccharification and fermentation (SSF) methods, respectively. The isoprene production via SSF had a 9.8% glucose–isoprene conversion which was equivalent to 98.8% of isoprene production via the pure glucose fermentation.ConclusionsThe optimized phosphoric acid/hydrogen peroxide combination pretreatment approach was proved effective to remove lignin and hemicellulose from lignocellulosic materials. Meanwhile, the pretreated PH could be converted into isoprene efficiently in the engineered Escherichia coli. It is concluded that this novel strategy of isoprene production using lignocellulosic materials pretreated by phosphoric acid/hydrogen peroxide is a promising alternative to isoprene production using traditional way which can fully utilize non-renewable fossil sources.

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Section: Resultscontrasting

confidence: 39%

Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method

Wang

et al. 2017

Biotechnol Biofuels

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“…Table 2 lists the solid yield; the main chemical composition of the pretreated substrates, including the content of cellulose, hemicellulose, and lignin; the cellulose recovery; and the removal of hemicellulose and lignin. The solid yield of these three boards ranged from 39.1% to 50.0%, which was close to that of wheat straw after PHP pretreatment [17]. It was apparent that PHP pretreatment enriched the cellulose fraction by easily removing the recalcitrant fractions of hemicellulose and lignin.…”

Section: The Main Fractions Of Furniture Boards Before and After Php Pretreatmentmentioning

confidence: 51%

“…Eight grams of furniture boards (dry basis) and 80.0 g of PHP solution were mixed in a 250 mL serum bottle. The bottle was shaken at 180 rpm, the optimized pretreatment temperature and duration were 40.2 • C and 2.9 h, respectively [17], and the pretreatment for each waste board was performed in duplicate. According to Figure S1, when pretreatment was complete, 200 mL of ethanol was added to facilitate the subsequent filtration.…”

Section: The Php Pretreatment Processmentioning

confidence: 99%

“…It appeared that fiberboard was mainly composed of crop straw, while chipboard and blockboard were mainly composed of sawdust or wood chips, since herbaceous biomass likely exhibits more extractives compared with woody biomass [24]. To give a fair comparison, the same pretreatment conditions were selected for these three feedstocks (phosphoric acid concentration of 79.6% and H 2 O 2 concentration of 1.9% for the PHP solution), which were the optimized conditions determined in a previous work [17]. Table 2 lists the solid yield; the main chemical composition of the pretreated substrates, including the content of cellulose, hemicellulose, and lignin; the cellulose recovery; and the removal of hemicellulose and lignin.…”

Section: The Main Fractions Of Furniture Boards Before and After Php Pretreatmentmentioning

confidence: 99%

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Valorizing Waste Lignocellulose-Based Furniture Boards by Phosphoric Acid and Hydrogen Peroxide (Php) Pretreatment for Bioethanol Production and High-Value Lignin Recovery

et al. 2019

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Three typical waste furniture boards (fiberboard, chipboard, and blockboard) were pretreated with phosphoric acid and hydrogen peroxide (PHP). The fractionation process of these feedstocks was attempted in order to harvest the cellulose-rich fraction for enzymatic hydrolysis and bioethanol conversion; further, lignin recovery was also considered in this process. The results indicated that 78.9–91.2% of the cellulose was recovered in the cellulose-rich fraction. The decreased crystallinity, which promoted the water retention capacity and enzyme accessibility, contributed greatly to the excellent hydrolysis performance of the cellulose-rich fraction. Therefore, rather high cellulose–glucose conversions of 83.3–98.0% were achieved by hydrolyzing the pretreated furniture boards, which allowed for harvesting 208–241 g of glucose from 1.0 kg of feedstocks. Correspondingly, 8.1–10.4 g/L of ethanol were obtained after 120 h of simultaneous saccharification and fermentation. The harvested lignin exhibited abundant carboxyl –OH groups (0.61–0.67 mmol g−1). In addition, approximately 15–26 g of harvested oligosaccharides were integrated during PHP pretreatment. It was shown that PHP pretreatment is feasible for these highly recalcitrant biomass board materials, which can diversify the bioproducts used in the integrated biorefinery concept.

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“…At the same time, the residual phosphorus in the wastewater could be removed using biochar adsorption to reduce phosphorus emissions [ 6 ]. At the optimized conditions, rather high cellulose hydrolysis and bioethanol conversion were achieved using simultaneous saccharification and fermentation process (SSF), e.g., 15.5 g ethanol was produced from 100 g wheat straw at rather high solid loadings [ 14 , 17 ]. It has been evidenced the strong oxidation reaction that occurred in PHP pretreatment system played an important role in biomass deconstruction and delignification to ease cellulose hydrolysis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Self-generated peroxyacetic acid in phosphoric acid plus hydrogen peroxide pretreatment mediated lignocellulose deconstruction and delignification

Tian

Chen

Shen

et al. 2021

Biotechnol Biofuels

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Background Peroxyacetic acid involved chemical pretreatment is effective in lignocellulose deconstruction and oxidation. However, these peroxyacetic acid are usually artificially added. Our previous work has shown that the newly developed PHP pretreatment (phosphoric acid plus hydrogen peroxide) is promising in lignocellulose biomass fractionation through an aggressive oxidation process, while the information about the synergistic effect between H3PO4 and H2O2 is quite lack, especially whether some strong oxidant intermediates is existed. In this work, we reported the PHP pretreatment system could self-generate peroxyacetic acid oxidant, which mediated the overall lignocellulose deconstruction, and hemicellulose/lignin degradation. Results The PHP pretreatment profile on wheat straw and corn stalk were investigated. The pathways/mechanisms of peroxyacetic acid mediated-PHP pretreatment were elucidated through tracing the structural changes of each component. Results showed that hemicellulose was almost completely solubilized and removed, corresponding to about 87.0% cellulose recovery with high digestibility. Rather high degrees of delignification of 83.5% and 90.0% were achieved for wheat straw and corn stalk, respectively, with the aid of peroxyacetic acid oxidation. A clearly positive correlation was found between the concentration of peroxyacetic acid and the extent of lignocellulose deconstruction. Peroxyacetic acid was mainly self-generated through H2O2 oxidation of acetic acid that was produced from hemicellulose deacetylation and lignin degradation. The self-generated peroxyacetic acid then further contributed to lignocellulose deconstruction and delignification. Conclusions The synergistic effect of H3PO4 and H2O2 in the PHP solvent system could efficiently deconstruct wheat straw and corn stalk lignocellulose through an oxidation-mediated process. The main function of H3PO4 was to deconstruct biomass recalcitrance and degrade hemicellulose through acid hydrolysis, while the function of H2O2 was to facilitate the formation of peroxyacetic acid. Peroxyacetic acid with stronger oxidation ability was generated through the reaction between H2O2 and acetic acid, which was released from xylan and lignin oxidation/degradation. This work elucidated the generation and function of peroxyacetic acid in the PHP pretreatment system, and also provide useful information to tailor peroxide-involved pretreatment routes, especially at acidic conditions. Graphical abstract

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Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification

Cited by 22 publications

References 31 publications

Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method

Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method

Valorizing Waste Lignocellulose-Based Furniture Boards by Phosphoric Acid and Hydrogen Peroxide (Php) Pretreatment for Bioethanol Production and High-Value Lignin Recovery

Self-generated peroxyacetic acid in phosphoric acid plus hydrogen peroxide pretreatment mediated lignocellulose deconstruction and delignification

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