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Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP), in which temperature, time, and HPO proportion for pretreatment were investigated by using response surface method. Results indicated that hemicellulose and lignin removal positively responded to the increase of pretreatment temperature, HPO proportion, and time. HPO proportion was the most important variable to control cellulose recovery, followed by pretreatment temperature and time. Moreover, these three variables all negatively related to cellulose recovery. Increasing HPO proportion can improve enzymatic hydrolysis; however, reduction on cellulose recovery results in decrease of glucose yield. Extra high temperature or long time for pretreatment was not beneficial to enzymatic hydrolysis and glucose yield. Based on the criterion for minimizing HPO usage and maximizing glucose yield, the optimized pretreatment conditions was 40 °C, 2.0 h, and HPO proportion of 70.2 % (HO proportion of 5.2 %), by which glucose yielded 299 mg/g wheat straw (946.2 mg/g cellulose) after 72-h enzymatic hydrolysis.

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