“…For many species, this stage is determinant for the success of the commercial production of micropropagated plantlets, since, in the laboratory, they are under an environment with high relative humidity, reduced light intensity, limited gas exchange and constant temperature and photoperiod, conditions very different from when transplanted to the natural environment. Accordingly, plants developed in vitro become heterotrophic and may exhibit limited structural and functional characteristics and, when subjected to acclimatization, are exposed to moderate or severe environmental stress (Van Huylenbroeck & Debergh 1996, Luis et al 2010, Mishra et al 2011, Singh et al 2013, Gomes et al 2015. Moreover, for bamboo, there is also the heterogeneous size of the plants and the number of clusters aggregated to the plantlets when they are taken to acclimatization, which may interfere in the transpiration, energy metabolism, vigor and emission of new shoots during the ex vitro stage, characteristics that may quickly lead to the plant death under greenhouse conditions.…”