Optimizing SIC assay method for acetyl salicylic acid and rosuvastatin and adapting to HPLC with performance comparison

Abstract

Summary. Acetyl salicylic acid (ASA), as the major drug consumed in the world, has been combined with other various drugs. In this regard, ASA-rosuvastatin is the most recent combination. The current article reported the first two separation methods for the assay of that combination utilizing sequential injection chromatography (SIC) and highperformance liquid chromatography (HPLC). The optimization process was conducted for SIC, and the optimum conditions were then adapted to HPLC. Both methods, SIC and HPLC, were validated and successfully applied to tablet formulation. In both methods, the analytes were chromatographed onto a short C18 monolithic column (4.6 × 25 mm) by a mobile phase composition of 10 mmol L −1 phosphate:acetonitrile: methanol (50:30:20, v/v/v at pH 3.0). The performances of both methods were compared. The remarkable advantages of the SIC method over HPLC method were the reduction in reagent consumption and instrumentation simplicity and cost-effectiveness. The total volume of mobile phase consumed is three orders of magnitude larger in HPLC than that in SIC. Other analytical features of the SIC and HPLC methods were comparable and acceptable for pharmaceutical analysis. Accordingly, the SIC method could be more suitable for routine analysis of simple matrices after examining the capability of the technique to work under industrial conditions.

Facile assay method for norfloxacin and ciprofloxacin by sequential injection chromatography

“…Because it is the most effective condition on separation efficiency, the composition of mobile phase was optimized using the factorial design approach [37][38][39][40][41][42]. To simplify the matrix of a factorial design, it is desirable to minimize conditions as possible [37][38][39][40][41][42].…”

“…To simplify the matrix of a factorial design, it is desirable to minimize conditions as possible [37][38][39][40][41][42]. Based on a previous study [18], water-acetonitrile-triethylamine (30/70/0.3, v/v/v at pH 3.3) recorded primarily acceptable separation, but not sufficient.…”

“…The resolution, retention time, and peak height were determined (Table I). The main and the interaction effect factors of the examined conditions on the adopted responses were calculated [37][38][39][40][41][42]. The obtained results are depicted in Fig.…”

Utilitization of Charge Transfer Complexation Reaction for the Spectrophotometric Determination of Vardenafil HCl and Yohimbin HCl in Pharmaceutical Formulations

“…The chemical structure of the studied drugs There is no official method for the determination of VARD in its formulations. Few reports have been published on the determination of Vd, including chromatographic methods [5][6][7][8][9][10][11][12] , gas chromatography 13,14 , electrokinetic capillary chromatography 15,16 , electrochemical methods 17,18 and atomic emission spectrometry 19 .…”

Advances in the Hyphenation of Flow Analysis Techniques with Liquid Separations for Pharmaceutical Analysis

“…FIC, SIC and MSC offer distinct advantages in terms of rapidity, simplicity, versatility and capital costs compared with high performance liquid chromatography (HPLC) and have been applied to the determination of a large number of pharmaceuticals [3][4][5]. When simple mixtures are considered, these hyphenated methods produce results that are comparable to HPLC [8,9]. The main advances in the hyphenation of flow analysis techniques with liquid separations for the purposes of pharmaceutical analysis are identified below.…”