Optimizing the Extraction of Anti-tumor Polysaccharides from the Fruit of Capparis spionosa L. by Response Surface Methodology

Abstract

Capparis spionosa L. is a traditional medicinal plant in China and central Asia. In this study, an experiment was designed to investigate the optimization of the extraction of anti-tumor polysaccharides from the fruit of Capparis spionosa L. (CSPS) by response surface methodology (RSM). Four independent variables (extraction temperature, extraction time, ratio of water to sample and extraction cycles) were explored. Meanwhile, the in vivo anti-tumor activity of CSPS was investigated. The results showed that the experimental data could be fitted to a second-order polynomial equation using multiple regression analysis. The optimum extraction conditions were as follows: extraction temperature 92 °C, extraction time 140 min, ratio of water to sample 26 mL/g, and three extraction cycle. Under these conditions, the yield of polysaccharides reached 13.01%, which was comparable to the predicted yield (12.94%, p > 0.05). This indicated that the model was adequate for the extraction process. Additionally, CSPS could prolong the survival time of H22 bearing mice in vivo. The anti-tumor activities of CSPS were dose-dependent.

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

“…Response surface methodology is a collection of statistical and mathematical techniques for optimization study [20] and is widely used to optimize conditions for extracting active compounds from herbs [21,22]. The main advantage of RSM is to reduce the number of experiments necessary to conduct the research.…”

Anti-tumor effect of polysaccharides isolated from <i>Taraxacum mongolicum</i> Hand-Mazz on MCF-7 human breast cancer cells

“…Response surface methodology (RSM) is an effective model for optimizing complex processes, and it saves more labor and time than other methods [13]. RSM is easy to carry out, and it has already been applied to optimize the extraction conditions of polysaccharide in previous researches [14,15].…”

“…More importantly, a growing amount of researches have shown that polysaccharides could resist tumors by inducing tumor apoptosis [6,7]. Taraxacum mongolicum Hand.-Mazz., a member of genus Taraxacum, is a folk medicine which was used to treat viral infectious diseases and inflammatory disorders, etc.…”

Response Surface Optimized Extraction of Total Triterpene Acids from <i>Eriobotrya japonica</i> (Thunb) <i>Lindl</i> (Loquat) Leaf and Evaluation of their In vitro Antioxidant Activities

“…Taking into account industry operating convenience [14], the confirmatory experiment was tested under the conditions: ethanol concentration 81%, ratio of solvent to sample 14 mL/g, extraction time 160min. A mean value of yields of TTA (3.41 ± 0.07 %, n = 3) was obtained, and the difference between the real value and the predicted value was not significant (p > 0.05).…”