Optimizing the lipid profile, to produce either a palm oil or biodiesel substitute, by manipulation of the culture conditions for<i>Rhodotorula glutinis</i>

Sargeant, Lisa A.; Chuck, Christopher J.; Donnelly, Joseph; Bannister, Christopher D; Scott, Roderick J.

doi:10.4155/bfs.13.64

Cited by 19 publications

(23 citation statements)

References 37 publications

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“…The other fatty acids were stearic acid (C18:0) (8.3 %), alpha-linolenic acid (C18:3) (5.9 %) and myristic acid (C14:0) (2.6 %). The fatty acid composition of R.glutinis SO28 was similar to those of other R.glutinis strains reported by previous studies [39][40][41]. The Table 4 Effect of additional phosphorus source on lipid accumulation in R. glutinis Waste Biomass Valor most prominent fatty acid of R. glutinis S028 was oleic acid.…”

Section: Determination Of Fatty Acids Composition Of the Yeastsupporting

confidence: 74%

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

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et al. 2016

Waste Biomass Valor

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This study was performed to produce lipids from the isolated oleaginous yeast Rhodotorula glutinis SO28 using loquat kernel extract (LKE) as substrate. LKE was prepared using acid hydrolysis and alkaline neutralization steps. Lipid production was performed in shaking flaks culture. Even if LKE was used as a sole source of nutritional substances, it could support cell growth and lipid synthesis in the yeast. Additional carbon, nitrogen and phosphorus sources were found to significantly alter the lipid accumulation potential of the yeast. Optimal concentrations of additional carbon (glucose) and nitrogen (ammonium sulphate) sources for lipid accumulation were determined as 15 and 0.5 g/L, respectively. On the other hand, all the concentrations of additional phosphorus source were found to significantly reduce the lipid accumulation. Optimal incubation time was determined as 132 h. Under the optimized culture conditions, the lipid concentration and lipid content of the yeast were determined as 7.82 g/L and 62 %, respectively. Fatty acid methyl ester analysis exhibited that this yeast strain could produce high proportions of C16:0 and C18 fatty acids, which are ideal for biodiesel production. This is the first report on the use of waste loquat kernels as substrate for microbial lipid production.

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Section: Determination Of Fatty Acids Composition Of the Yeastsupporting

confidence: 74%

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

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Yazıcı

Taşkın

et al. 2016

Waste Biomass Valor

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“…This requires selecting a feedstock with a high hemicellulose content – in the case of the popular leaves used in this study, 80–85% of their dry weight . Previously, Rhodotorula glutinis has been demonstrated to produce an oil akin to rapeseed oil or palm oil depending on the environmental conditions used; however on the lignocelluose hydrolyzate the strain used produced a lipid with 70% monounsaturated esters, more akin to olive oil than palm.…”

Section: Suitability Of Oleaginous Yeasts For Industrial Production Omentioning

confidence: 99%

“…R. toruloides lipid, for example, could be made significantly more saturated through altering the carbon‐to‐sulfur (C/S) ratio of the growth medium . Alternatively, Zygomycete sp., C. curvatus, C. oleophila, C. utilis, R. glutinis and R. toruloides could be made to produce more saturated lipids by altering the temperature of the fermentation . However, some oleaginous yeasts, such as R. minuta are not susceptible to changes in the lipid profile irrespective of the environmental conditions employed …”

Section: Suitability Of Oleaginous Yeasts For Industrial Production Omentioning

confidence: 99%

Toward a microbial palm oil substitute: oleaginous yeasts cultured on lignocellulose

Whiffin

Santomauro

Chuck

2016

Biofuels Bioprod Bioref

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Palm oil production is a leading contributor to tropical deforestation, resulting in habitat destruction, increased carbon dioxide emissions, and local smog clouds across South East Asia. Palm oil is widely used for food, as a biofuel precursor, and in soaps and cosmetics. The global demand for palm oil is approximately 57 m tonnes a−1 and is steadily increasing. Alternatively, oleaginous yeast offers a highly credible renewable substitute. Over 80 species of oleaginous yeast are known, many of which have been demonstrated to catabolize a wide range of mono‐ and oligosaccharides in lignocellulose hydrolyzates. Many of the yeasts have demonstrated a high tolerance to furfurals and organic acid inhibitors and can be cultured at low pH, ideal for the industrial production of oil from lignocellulosic sources such as stover, forestry wastes, or energy crops. While the majority of the yeasts produce predominantly monounsaturated esters, some species are capable of producing oils higher in saturates akin to palm oil. While the manufacture of yeast oils on an industrial scale has been demonstrated, currently no large‐scale production from lignocellulose exists; however, through further strain selection, metabolic engineering, and valorization of co‐products, the production of a substitute oil from yeast is potentially feasible. © 2016 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…La eficiencia de la temperatura programada en la ILCA se observó en los resultados del crecimiento de Rodosporidium turoloides, las que fueron incubadas a 30 °C, cuyo recuento en placa de las respectivas diluciones se muestran en la Tabla 1, denotándose que no existieron diferencias significativas (prueba t para medias de dos muestras emparejadas, p > 0,05) en el desarrollo de Rodosporidium turoloides utilizando la ILC Memmert y la ILCA, demostrando la eficiencia de control de la temperatura en esta última. Sargeant et al, (2014), reportan que la temperatura óptima para la producción de biomasa de levaduras oleaginosas, se encuentra en el intervalo de 25 a 30 °C. Costos para la modificación del HED a ILCA: Se utilizó los componentes indicados en la Tabla 2, observándose que el costo total del ILCA de S/ 608,74 es menor comparado con los ILC.…”

Section: Resultados Y Discusiónunclassified

Modification of a domestic electric oven to a controlled laboratory incubator with Arduino

Herrera-German¹,

Mercedes-Chávez²,

Vásquez-Villalobos³

2018

Agroind.sci.

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RESUMENTeniendo en cuenta que un Horno Eléctrico Doméstico (HED) THOMAS TH-42NO2 tiene un costo de $141,87 USD (S/ 458,24) y que su estructura y algunos elementos pueden ser utilizados, se modificó a Incubadora de Laboratorio Controlada con Arduino (ILCA) y su funcionamiento fue probado experimentalmente, con la incubación en medio de cultivo de un microorganismo base (Rodosporidium turoloides), sensible a la temperatura. Se comprobó que no existieron diferencias significativas (p > 0,05) en su crecimiento utilizando una Incubadora de Laboratorio Comercial (ILC) y la ILCA; demostrando la eficiencia de control de la temperatura. El costo total de la ILCA fue de S/ 608,74, menor que los ILC; cuyo costo fluctúa para una unidad de similar tamaño en S/ 10 723,60. Lo que justifica económicamente la modificación de un HED a una ILCA para fines de trabajos de laboratorio.Palabras clave: horno electrico; incubadora de laboratorio; Arduino; control automatico; Rodosporidium turoloides. ABSTRACTTaking into account that a THOMAS TH-42NO2 Domestic Electric Oven (DEO) has a cost of $ 141.87USD (S/ 458.24) and that its structure and some elements can be used, it was modified to Arduino Controlled Laboratory Incubator (ACLI) and its operation was experimentally tested, with the incubation in a culture medium of a base microorganism (Rodosporidium turoloides), sensitive to temperature. It was found that there were no significant differences (p>0.05) in their growth using a Commercial Laboratory Incubator (CLI) and the ACLI; demonstrating the efficiency of temperature control. The total cost of the ACLI was S/ 608.74, lower than the CLI; whose cost fluctuates for a unit of similar size in S/ 10 723.60. What economically justifies the modification of a DEO to an ACLI for the purpose of laboratory work.Keywords: electric oven; laboratory incubator; Arduino; automatic control; Rodosporidium turoloides. IntroducciónLa importancia de los laboratorios, tanto en la enseñanza como en la investigación, es sin duda alguna indiscutible. El trabajo práctico en laboratorio proporciona la oportunidad de la experimentación y el descubrimiento, evitando el concepto de "resultados correctos" sólo con los datos procedentes de textos. En términos generales, un laboratorio es un lugar equipado con diversos instrumentos de medición, entre otros, donde se realizan experimentos o investigaciones diversas, según la rama de la ciencia a la que se enfoque (Lugo, 2006). Acreditation Board for Engineering and Technology (ABET) considera como requisitos del laboratorio: la realización de un trabajo "apropiado" en todos los planes de estudios, así como la combinación de elementos teóricos y prácticos. Si esto se hace en forma apropiada incluirá mediciones, análisis y aspectos de diseño, así como el proceso ordenado y lógico para producir resultados válidos. Un laboratorio bien diseñado es una valiosa herramienta que contribuye a reforzar la enseñanza y en el que los alumnos pueden lograr una mayor comprensión, imposible de lograr por otros medios. Allí, puede...

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Optimizing the lipid profile, to produce either a palm oil or biodiesel substitute, by manipulation of the culture conditions forRhodotorula glutinis

Cited by 19 publications

References 37 publications

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

Toward a microbial palm oil substitute: oleaginous yeasts cultured on lignocellulose

Modification of a domestic electric oven to a controlled laboratory incubator with Arduino

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