Optimizing the Properties of the Protein Corona Surrounding Nanoparticles for Tuning Payload Release

Abstract

We manipulate the passive release rates of DNA payloads on protein coronas formed around nanoparticles (NPs) by varying the corona composition. The coronas are prepared using a mixture of hard and soft corona proteins. We form coronas around gold nanorods (NRs), nanobones (NBs), and carbon nanotubes (CNTs) from human serum (HS) and find that tuning the amount of human serum albumin (HSA) in the NR-coronas (NR-HS-DNA) changes the payload release profile. The effect of buffer strength, HS concentration, and concentration of the cetyltrimethylammonium bromide (CTAB) passivating the NP surfaces on passive release is explored. We find that corona properties play an important role in passive release, and concentrations of CTAB, HS, and phosphate buffer used in corona formation can tune payload release profiles. These advances in understanding protein corona properties bring us closer toward developing a set of basic design rules that enable their manipulation and optimization for particular biological applications.

Exploring the diameter and surface dependent conformational changes in carbon nanotube-protein corona and the related cytotoxicity

“…Distinct configuration of the adsorbed protein was observed compared to the blurred ones in Fig. 2C and D, confirming the existence of stronger bindings and firmer adsorption of BSA onto the surface of CNTs with a smallerdiameter [15,16]. DLS measurements showed increased hydrated radius after adding the protein to CNTs (98.7-125 nm for <10 nm group, 145.3-171.7 nm for 10-20 nm group, and 203.1-219.8 nm for 20-40 nm group).…”

Magnetic field responsive drug release from magnetoliposomes in biological fluids

“…On the other hand, recent studies have shown that the presence of a protein corona could enhance the drug delivery action of NPs, promoting high payloads. Cifuentes-Rius et al 33 have shown that the payload release profile of pre-formed NP-protein corona complexes (nanorods, gold nanobones, and carbon nanotubes) is strongly affected by different biological environments. 33 In particular, fluids rich of hard corona proteins promoted a faster release of the payload than those bearing soft corona proteins.…”

“…Cifuentes-Rius et al 33 have shown that the payload release profile of pre-formed NP-protein corona complexes (nanorods, gold nanobones, and carbon nanotubes) is strongly affected by different biological environments. 33 In particular, fluids rich of hard corona proteins promoted a faster release of the payload than those bearing soft corona proteins. Here, we report a detailed study about the behaviour of MLs dispersed in serum upon exposure to a LF-AMF in order to understand how the magnetically triggered release is influenced by the interaction with serum proteins.…”

Multifunctional and robust composite materials comprising gold nanoparticles at a spherical polystyrene particle surface

“…These AuNPs display unique optical, mechanical, electrical and chemical properties, 3 which have been widely applied in catalysis, 4 surface enhanced Raman spectroscopy (SERS), 5 and nanomedicine. 6 In the area of nanomedicine they have been employed in light activated release of therapeutic payloads by NP heating, 7 and AuNP radiosensitization, which is a novel approach to enhance the ionising radiation dose in radiotherapy.…”