Optimum conditions for inducing laccase production in Lentinus crinitus

Valle, Juliana Silveira do; Vandenberghe, Luciana Porto de Souza; Santana, Thiago Teodoro; Almeida, P. H. de; Pereira, Ana Margarida; Linde, Giani Andréa; Colauto, Nelson Barros; Soccol, Carlos Ricardo

doi:10.4238/2014.october.20.31

Cited by 29 publications

(31 citation statements)

References 17 publications

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“…Whereas in case of Schizophyllum commune laccase, rice straw showed maximum production after 72 h of incubation (Adejoye and Fasidi, 2010). Also, Lentinus crinitus laccase showed enhanced production due to copper and phenolic compounds under optimized condition (Valle et al, 2014). Laccase from Ascomycete mauginiella was found to be optimum at 40°C (Palonen et al, 2003) and 30°C for B. subtilis WD23 laccase (Wang et al, 2013) which was consistent with current results.…”

Section: Discussionsupporting

confidence: 81%

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Narayanan¹,

Sevanan²,

Eva³

et al. 2015

Research J. of Microbiology

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Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are polyphenol oxidases (PPO) that catalyze the oxidation of various substituted phenolic compounds by using molecular oxygen as the electron acceptor. In the present study, Bacillus subtilis MTCC 2414 were employed for the production of laccase using guaiacol as a substrate under Submerged Fermentation (SmF) condition. Laccase was partially purified by salt precipitation method followed by dialysis. The synthetic dyes such as Orange 3R, Yellow GR and T-Blue were used for degradation studies using culture filtrate, free and immobilized laccases. The immobilized laccase obtained by entrapment method using sodium alginate was optimized and it exhibited maximum activity at pH 7 (321 U mLG 1 ) and temperature 35°C (317 U mLG 1 ). Similarly, free laccase was also optimized and the maximum activity was observed at pH 9 (309 U mLG 1 ) and temperature 35°C (339 U mLG 1 ). Surprisingly, Yellow GR dye was found to be highly degraded up to 81.72% by immobilized laccase when compared to free laccase (74.69% of T-Blue) and culture filtrate (72.16% of Orange 3R) respectively. The FTIR spectrum showed a spectrum peaks at 2110.12 c/m in control dye and degraded Yellow GR exposed peak at 3406.36 cmG 1 which represents C-N stretching of aromatic amine group in dyes. Therefore, high degradation ability of laccase from B. subtilis MTCC 2414 makes them attractive catalyst for biotechnological applications.

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Section: Discussionsupporting

confidence: 81%

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Narayanan¹,

Sevanan²,

Eva³

et al. 2015

Research J. of Microbiology

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“…According to Piscitelli et al (2011), non-protein nitrogen sources reduce laccase production, whereas protein sources increase enzyme production. In our study, the maximum laccase production of 57,067 U/L by P. sanguineus (using urea) was approximately 52 times greater than that by P. cinnabarinus using ammonium sulfate (Eggert et al, 1996), 127 times greater than that by P. sanguineus using ammonium tartrate (Pointing and Vrijmoed, 2000), and four times greater than that by Lentinus crinitus using urea (Valle et al, 2014b). Therefore, P. sanguineus is an important genetic resource, although other cultivation conditions besides genetic breeding should be studied to improve laccase production.…”

Section: Discussionmentioning

confidence: 43%

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. Laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. We evaluated laccase production in a recently isolated strain of P. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast urea) increased enzymatic activity. The addition of up to 300 µM of Cu did not affect laccase production, whereas cultivation with agitation increased the activity peak by 17%. The highest laccase activity was ~50,000 U/L on the ninth day of cultivation. After 24 h, decolorization was 80% for Remazol Brilliant Blue R, 9% for Reactive Yellow 145, 6% for Reactive Red 195, and 2% for Reactive Black 5. The enzymatic extract of P. sanguineus provides a potential alternative to wastewater treatment. A better understanding of the behavior of this fungus under various culture conditions would allow improvement of the enzyme production bioprocess.

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“…Higher nitrogen concentration increases laccase production by Pleurotus ostreatus and Lentinula edodes but decreases laccase production by Phanerochaete chrysosporium, Phlebia brevispora, Coriolus versicolor, and Pholiota mutabilis (Leatham and Kirk, 1983). Laccase production by Lentinus crinitus increases in the presence of 2.8 g/L nitrogen (Valle et al, 2014b) while that by P. ostreatus increases in the presence of 0.23 g/L nitrogen (Hou et al, 2004). The results of the present study and those of other studies (Ullrich et al, 2005;D'Agostini et al, 2011;Valle et al, 2014a) suggest that nitrogen concentrations in the range of 1.5-3.0 g/L induce maximum laccase production by A. blazei.…”

Section: Effect Of Nitrogen and Copper Concentrations On Laccase And mentioning

confidence: 96%

Effect of different compounds on the induction of laccase production by Agaricus blazei

Valle¹,

Vandenberghe²,

Oliveira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Laccases are polyphenol oxidases produced by many fungi and have many applications in textile, food and beverage, and pulp and paper industries. Laccase production can be induced using aromatic or phenolic compounds that mostly affect the transcription of laccase-encoding genes. In this study, we analyzed laccase and biomass production by Agaricus blazei in the presence of different concentrations of nitrogen, copper, and inducers such as pyrogallol, veratryl alcohol, xylidine, vanillin, guaiacol, and ethanol. Laccase production by A. blazei U2-4 reached 43.8 U/mL in the presence of 2.8 g/L nitrogen and 150 µM copper. However, addition of copper to the cultivation medium decreased biomass production. Different compounds differentially induced laccase production by A. blazei. Moreover, different concentrations of these inducers exerted different effects on laccase activity. Ethanol (1.0 mM), guaiacol (0.5 mM), and vanillin (0.5 mM) were the best inducers and increased laccase activity by 120% (A. blazei U2-2), 30% (A. blazei U2-3), and 9% (A. blazei U2-4), 15883Induction of laccase production by Agaricus blazei ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15882-15891 (2015) respectively. In contrast, pyrogallol and xylidine decreased laccase activity but increased biomass production.

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Optimum conditions for inducing laccase production in Lentinus crinitus

Cited by 29 publications

References 17 publications

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Effect of different compounds on the induction of laccase production by Agaricus blazei

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