Optimum parameters for production of ethanol from synthetic molasses by Saccharomyces cerevisiae

Reddy, Prashanth; Vijay, M.; Kusuma, Maria Erviana; Ramesh, K.

doi:10.1016/j.matpr.2020.07.100

Cited by 2 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a major by-product of sugar manufacturing process, molasses contains approximately 50% fermentable sugars and a small number of nitrogenous compounds, inorganic salts, and trace elements, which are the essential nutrients for growth and biosynthesis of S. cerevisiae [ 47 ] . The shake flask fermentation was conducted with an initial molasses concentration of 40 g/L.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae

Zhu

Peng

et al. 2022

Microb Cell Fact

View full text Add to dashboard Cite

Background Ginsenosides are Panax plant-derived triterpenoid with wide applications in cardiovascular protection and immunity-boosting. However, the saponins content of Panax plants is fairly low, making it time-consuming and unsustainable by direct extraction. Protopanaxadiol (PPD) is a common precursor of dammarane-type saponins, and its sufficient supply is necessary for the efficient synthesis of ginsenoside. Results In this study, a combinational strategy was used for the construction of an efficient yeast cell factory for PPD production. Firstly, a PPD-producing strain was successfully constructed by modular engineering in Saccharomyces cerevisiae BY4742 at the multi-copy sites. Then, the INO2 gene, encoding a transcriptional activator of the phospholipid biosynthesis, was fine-tuned to promote the endoplasmic reticulum (ER) proliferation and improve the catalytic efficiency of ER-localized enzymes. To increase the metabolic flux of PPD, dynamic control, based on a carbon-source regulated promoter PHXT1, was introduced to repress the competition of sterols. Furthermore, the global transcription factor UPC2-1 was introduced to sterol homeostasis and up-regulate the MVA pathway, and the resulting strain BY-V achieved a PPD production of 78.13 ± 0.38 mg/g DCW (563.60 ± 1.65 mg/L). Finally, sugarcane molasses was used as an inexpensive substrate for the first time in PPD synthesis. The PPD titers reached 1.55 ± 0.02 and 15.88 ± 0.65 g/L in shake flasks and a 5-L bioreactor, respectively. To the best of our knowledge, these results were new records on PPD production. Conclusion The high-level of PPD production in this study and the successful comprehensive utilization of low-cost carbon source -sugarcane molassesindicate that the constructed yeast cell factory is an excellent candidate strain for the production of high-value-added PPD and its derivativeswith great industrial potential. Graphical Abstract

show abstract

Section: Resultsmentioning

confidence: 99%

“…The production of PPD with glucose and molasses feeding achieved 841.09 ± 2.16 mg/L and 556.26 ± 4.90 mg/L, respectively. The lower PPD titer in molasses might be caused by the large quantities of ash and metal ions in molasses, which inhibit the synthesis of target compounds [ 47 ]. Then, two-stage feeding strategy was conducted.…”

Section: Resultsmentioning

confidence: 99%

High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae

Zhu

Peng

et al. 2022

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…According to the results, molasses showed the highest potential for use as a low-cost carbon-source for the enzyme production, which increased the SOD activity up to 1.58-fold when compared to the normal YPD (328.24 and 208.18 U/mL, respectively). In addition, molasses obtained from sugarcane mills represents a low-cost source of waste containing a high concentration of mono-and disaccharides such as glucose, sucrose and fructose (approximately 50%(w/w)), which microorganisms can utilize for their growth and producing the essential metabolites [31,32]. As for the other carbon-sources, fructose and sucrose were also good sources for cell biomass and SOD production due to the ability of the yeast cell to utilize those carbon-sources.…”

Section: Optimization Of Medium Composition For Sod Production At Fla...mentioning

confidence: 99%

Enhancing the Productivity and Stability of Superoxide Dismutase from Saccharomyces cerevisiae TBRC657 and Its Application as a Free Radical Scavenger

et al. 2022

View full text Add to dashboard Cite

Superoxide dismutase (SOD) is crucial antioxidant enzyme that plays a role in protecting cells against harmful reactive oxygen species (ROS) which are generated inside cells. Due to its functionality, SOD is used in many applications. In this study, Saccharomyces cerevisiae TBRC657 was selected as the SOD producer due to its high SOD production. After investigating an optimized medium, the major components were found to be molasses and yeast extract, which improved SOD production up to 3.97-fold compared to a synthetic medium. In addition, the optimized medium did not require any induction, which makes it suitable for applications in large-scale production. The SOD formulation was found to increase the stability of the conformational structure and prolong shelf-life. The results show that 1.0% (w/w) trehalose was the best additive, in giving the highest melting temperature by the DSF method and maintaining its activity at more than 80% after storage for 6 months. The obtained SOD was investigated for its cytotoxicity and ROS elimination against fibroblast cells. The results indicate that the SOD enhanced the proliferation and controlled ROS level inside the cells. Thus, the SOD obtained from S. cerevisiae TBRC657 cultured in the optimized medium could be a candidate for use as a ROS scavenger, which can be applied in many industries.

show abstract

Optimum parameters for production of ethanol from synthetic molasses by Saccharomyces cerevisiae

Cited by 2 publications

References 5 publications

High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae

High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae

Enhancing the Productivity and Stability of Superoxide Dismutase from Saccharomyces cerevisiae TBRC657 and Its Application as a Free Radical Scavenger

Contact Info

Product

Resources

About