Optisol Containing Streptomycin

The Effect of Temperature on the Antimicrobial Activity of Optisol-GS

“…Because donor corneas are often removed in hospitals and other settings where resistant bacteria may be thriving, the addition of other antibiotics should be considered. Cephalosporins, 2,5,33 penicillin G, 24,33 and vancomycin 23 have been suggested as possible candidates and have not been shown to have a deleterious effect on corneal structure or endothelial cells.…”

Endophthalmitis Caused byCitrobacterSpecies

“…In general, gentamicin and streptomycin have decreased activity at 4 • C compared with their predicted activity at 37 • C, and therefore it is necessary to leave the cornea in the storage media at room temperature for 3 h prior to refrigeration at 4 • C. 31 It is best to warm the cornea and storage media to room temperature for 1 h prior to the scheduled procedure. 32 They may reduce donor-to-host transmission of bacterial infection causing endophthalmitis after penetrating keratoplasty.…”

Preserving the cornea: corneal storage media

“…The addition of streptomycin to Optisol resulted in the creation of Optisol GS [13][14][15], currently the most widely used cold storage medium in the United States. In areas of the world where donor corneas are less available than they are in the United States, however, it is necessary to increase the donor pool by storing tissue for longer periods of time.…”

“…As is the case with antibiotics in general, however, gentamicin and streptomycin have decreased activity at 48C compared with their predicted activity at 378C, and therefore it is necessary to leave the cornea in the storage media at room temperature for 3 h prior to refrigeration at 48C [15]. In addition, it is best to warm the cornea and storage media to room temperature for 1 h prior to the scheduled procedure [13].…”