The Effect of Temperature on the Antimicrobial Activity of Optisol-GS

Abstract

The antimicrobial activity of Optisol-GS was reduced at refrigerated temperature and enhanced at RT. Bactericidal activity was not observed against E. faecium at either refrigerated temperature or RT.

Report of the Eye Bank Association of America Medical Review Subcommittee on Adverse Reactions Reported From 2007 to 2014

“…Conversely, the antibacterial activity of Optisol-GS is enhanced at room temperature, thus creating a noncompetitive environment allowing fungi to grow. 21 Reports suggest a high association between fungal infection and EK and tissue prepared in the eye bank, as compared with surgeon-prepared tissue. 15 Donor-to-host transmission of infection or malignancy is a rare but important consideration associated with corneal transplantation.…”

The Safety and Efficacy of Linezolid and Daptomycin as an Additive in Optisol-GS Against Methicillin-Resistant Staphylococcus aureus

“…Because the antimicrobial agents in the media work primarily on actively replicating or metabolically active microorganisms, they are more effective during and after the 3-hour warming period than at 4°C. 14, 39 Smith et al 12 also reported that the bactericidal activity of Optisol-GS against S. aureus was higher at 22°C, although gentamicin-resistant strains of S. aureus do not seem to have been included in this study.…”

Quality Assessment and Microbiologic Screening of Donor Corneas

“…We agree that a clinical observational study may help to determine the optimal maintenance of donor corneal preservation before keratoplasty. 15 Our observation that the quality rating of corneal tissue is associated with donor corneal rim culture results could be a chance finding or might be biased because only 48% of donor corneal rims were submitted for microbiologic processing. Our finding, however, is consistent with that of a recent study showing that stromal edema may allow bacteria to enter the donor cornea.…”