Optisol Corneal Storage Medium

Lindstrom, Richard L.; Kaufman, Herbert E.; Skelnik, Debra L.; Laing, Ronald A.; Lass, Jonathan H.; Musch, David C.; Trousdale, Melvin D.; Reinhart, William J.; Burris, Terry E.; Sugar, Alan; Davis, Richard M.; Hirokawa, Kiminori; Smith, Teresa; Gordon, Judy

doi:10.1016/s0002-9394(14)71803-3

Cited by 138 publications

(69 citation statements)

References 12 publications

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“…Another in vitro study reported thinner corneal tissue and better preservation of structural morphology of endothelium during Optisol storage. 3 Although the viability of the donor endothelium is far more important than the epithelium for the success of penetrating keratoplasty, the increasing use of donor corneal epithelium in cases of severe disturbances of epithelial integrity dictates a reexamination of storage effect on the epithelial layer.…”

Section: Optisol Vs Dexsol In Preservation Of Corneal Epitheliummentioning

confidence: 99%

Optisol vs Dexsol as storage media for preservation of human corneal epithelium

Greenbaum

Hasany

Rootman

2004

Eye

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Purpose To compare the efficacy of two storage media, Optisol GS and Dexsol, in preservation of donor corneal epithelium. Methods A total of 12 pairs of corneas not suitable for transplantation, all with intact epithelium, were used in this study, with one cornea of the pair stored in Optisol GS and its other counterpart in Dexsol. At each of three durations of storage -1, 2, and 4 days F four of these paired corneas were prepared for light microscopy and scanning and transmission electron microscopy. Another four pairs of control cornea were prepared in the same way and placed in universal fixative. Main outcome measures Evaluation of the corneas was made by two observers masked as to the identity of the storage medium and length of storage. Loss of epithelial cells was evaluated by light microscopy. The attachment of the epithelium to the basement membrane, cellular integrity, intercellular junctions, and intracellular organelles were evaluated and compared by electron microscopy. Results The magnitude of epithelial loss correlated with the length of storage time. Control corneas maintained normal epithelium with preservation of all epithelial cell layers. Corneas stored for 1 day had minimal damage of the epithelium. Corneas stored for 2 days had a slight increase in epithelial damage, and corneas stored for up to 4 days showed a marked increase in epithelial damage. There were no significant differences between the two storage media. The basal cell layer was maintained in both the media at all time points, usually in good condition with mild-to-moderate damage in some cases. Conclusions Loss of donor epithelium is related mainly to the length of storage and is similar in both Optisol GS and Dexsol. The storage time should be less than 4 days, especially when performing penetrating keratoplasty on patients with ocular surface disorders.

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Section: Optisol Vs Dexsol In Preservation Of Corneal Epitheliummentioning

confidence: 99%

Optisol vs Dexsol as storage media for preservation of human corneal epithelium

Greenbaum

Hasany

Rootman

2004

Eye

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“…It has been demonstrated that Optisol as compared with Dexsol, K-Sol, and MK medium, can preserve the corneal endothelium better than yielding a thinner cornea up to 2 weeks. 7 A thinner cornea permits a better evaluation and easier manipulation of the donor tissue at the time of the surgical procedure and also helps an earlier visual rehabilitation. Nelson and associates compared Chen medium with Optisol-GS in their study.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Yüksel

Uzunel²,

Küsbeci³

2016

Exp Clin Transplant

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Objectives: To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium. Materials and Methods: Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process. Results: Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm 2 at the beginning of the preservation (range, 1361-2899 cells/mm 2 ). Donor endothelial cell density was between 1500 to 2000 cells/mm 2 in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm 2 on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day. Conclusions: Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.

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“…Todas as córneas foram preservadas no meio Optisol GS  (Chiron Ophthalmics), meio baseado em sulfato de condroitina capaz de manter a viabilidade do tecido corneano por um período de até 14 dias (6) . Entretanto, estudos clínicos e experimentais têm demonstrado perda de células endoteliais após um período de sete a dez dias de preservação neste tipo de meio (7) .…”

Section: Discussionunclassified