OPTIXcell improves the postthaw quality and fertility of buffalo bull sperm

Ansari, Muhammad Tayyab; Rakha, Bushra Allah; Akhter, Shamim; Ashiq, M.

doi:10.1016/j.theriogenology.2015.09.035

Cited by 23 publications

(38 citation statements)

References 36 publications

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“…No differences between post-thawed sperm motility in semen which were extended with Optixcell® at 2 hours and 4 hours, Lima-verde et al (2017) revealed that sperm bull extended with Characteristics of Post-thawed Bali Bull Semen (A.S. Amal et al) 141 Optixcell® has improved mitochondrial membrane potential compared to soy-lecithin extender. Moreover, semen extended in Optixcell® had improved motility kinematic parameters than semen extended in Tris-cEY and Bioxcell® such a finding as previously suggested by Kumar et al (2015), Ansari et al (2016) and Murphy et al (2018). Liposomes behave similar to the cell membrane and adhere to the sperm membrane during cryopreservation, ensuring the exchange of cholesterol and phospholipids (Ansari et al, 2016).…”

Section: Discussionsupporting

confidence: 71%

“…Moreover, semen extended in Optixcell® had improved motility kinematic parameters than semen extended in Tris-cEY and Bioxcell® such a finding as previously suggested by Kumar et al (2015), Ansari et al (2016) and Murphy et al (2018). Liposomes behave similar to the cell membrane and adhere to the sperm membrane during cryopreservation, ensuring the exchange of cholesterol and phospholipids (Ansari et al, 2016). The protective activity of lecithin based extender could be due to the presence of a mixture of phosphatidylcholin and several fatty acids that protect sperm plasma membrane during freezing.…”

Section: Discussionsupporting

confidence: 63%

“…Bioxcell® is a commercially available soybean lecithin-based extender which used as a substitution for Egg Yolk but the results of using it for semen freezing are controversial. Another commercially available extender is Optixcell® which is synthetic liposome based extender; those liposomes are molecules which are characterized by being defined chemically, and having the ability to exchange lipids and cholesterol with the damaged portions of the sperm plasma membrane (Belala et al, 2016a;Ansari et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Amal

Arifiantini

Setiadi

et al. 2019

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan dari penelitian ini adalah untuk menguji efektivitas dari tiga macam bahan pengencer (Tris kuning telur, Bioxcell® dan Optixcell®) dan tiga waktu ekuilibrasi (T-30 min, T-2 jam dan T-4 jam) untuk kriopreservasi semen sapi bali. Tiga puluh enam ejakulat semen berasal dari 6 ekor sapi bali jantan dikoleksi dan dibekukan mengikuti protokol pembekuan rutin. Evaluasi motilitas sperma postthawing (PTM) dilakukan dengan computer assisted semen analysis (CASA). Integritas membran plasma dievaluasi dengan hypo-osmotic swelling (HOS) test dan viabilitas dievaluasi dengan pewarnaan eosin nigrosin. Hasil penelitian menunjukkan terdapat interaksi yang signifikan antara waktu ekuilibrasi dan pengencer terhadap motilitas, viabilitas dan integritas membran sperma sapi bali. Perlakuan kontrol (4 jam waktu ekuilibrasi) memiliki nilai tertinggi (P<0,05) dan perlakuan 30 menit waktu ekuilibrasi memiliki nilai terrendah (P<0,05) untuk total motilitas, motilitas progresif, persentase sperma dengan membran plasma utuh (MPU) dan viabilitas sperma. Motilitas, skor pergerakan individu dan integritas membran sperma dalam pengencer Optixcell® lebih tinggi dibandingkan dengan semen yang dibekukan dalam pengencer Bioxcell® dan Tris kuning telur. Motilitas total, progresif dan integritas membran sperma terbaik secara keseluruhan terdapat dalam pengencer Tris kuning telur dan Bioxcell® dengan waktu ekuilibrasi 4 jam dan Optixcell® dengan waktu ekuilibrasi 2 dan 4 jam. Kesimpulan berdasarkan analisis subyektif dan obyektif, equilibrasi 30 menit tidak cukup untuk mempertahankan motilitas dan integritas membran sperma yang lebih baik; equilibrasi untuk 2 jam menghasilkan motilitas sperma tertinggi untuk pengencer Optixcell®; dan equilibrasi untuk 4 jam menghasilkan viabilitas tertinggi untuk semua pengencer yang digunakan.Kata kunci: sapi bali, kriopreservasi semen, pengencer semen, waktu ekuilibrasi, CASA. ABSTRACTThe objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Trisclarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) andCharacteristics of Post-thawed Bali Bull Semen (A.S. Amal et al.) 135 Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P<0.05) for all the evaluated parameters independent of extender type. Overall, semen extended in Tris-cEY, Bioxcell® and O...

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Amal

Arifiantini

Setiadi

et al. 2019

J. Indonesian Trop. Anim. Agric.

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“…The efficacy of lecithin based extenders is still a matter of debate. Many previous studies have reported either comparable or higher sperm motility and plasma membrane and acrosomal integrity (Thun et al, 2002;Amirat et al, 2005;Rastegarnia et al, 2013;Singh et al, 2013;Chaudhari et al, 2015;Kumar et al, 2015;Ansari et al, 2016, Layek et al, 2016 with similar or even better fertility rates (Bousseau et al, 1998;Gil et al, 2000;Akhter et al, 2010;Akhter et al 2012;Beura et al, 2014) for bovine semen cryopreserved using lecithin or plant derived soya bean based commercial extenders, while some studies showed better efficiency of egg yolk based extenders over soya lecithin based extenders (Celeghini et al, 2008;Veerabramhaiah et al, 2011;Crespilho et al, 2012).…”

Section: Results and Discussion Sperm Quality Attributes During Cryopmentioning

confidence: 97%

“…Studies targeting to assess the efficacy of soy lecithin in egg yolk free semen extenders were performed in bovine (Aires et al, 2003;Muino et al 2007;Veerabramhaiah et al, 2011;Singh et al, 2013;Beura et al, 2014;Layek et al, 2016) and bubaline (Akhter et al, 2010;Meena et al, 2010;Ansari et al, 2016;Chaudhari et al, 2015Chaudhari et al, , 2017. However, results obtained using lecithin as substitute to egg yolk are still a matter of debate (Leite et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of egg yolk based and egg yolk free soya bean milk based extenders for cryopreservation of Zebu cattle and buffalo semen

Chaudhary

Dhami

Chaudhari

et al. 2018

IJAR

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This study was undertaken on three mature bulls each of Gir cattle and Surti buffalo breeds to evaluate the comparative efficacy of egg yolk based standard TFYG (Tris-citrate-fructose-yolk-glycerol) extender and egg yolk free soybean based commercial extenders Optixcell® (IMV, France) and Andromed® (Minitube, Germany) under split-sample technique. The ejaculates (9/bull) were extended @ 100×10 6 sperm ml -1 with three extenders and frozen using biofreezer following 4 hr of equilibration. The pooled means of progressively motile sperm observed (irrespective of extenders) at initial, pre-freeze and post-thaw stage in Gir bulls semen were 76.53±0. Optixcell was proved superior, and at par with TFYG, than the Andromed in maintaining greater motility, viability, morphology, acrosomal/plasma membrane integrity including post-thaw sperm longevity of cattle and buffalo spermatozoa with significant differences only in sperm motility and post-thaw longevity. The motile, live and HOST reactive sperm were significantly higher in buffalo semen than cattle at initial and pre-freeze stage, but not at post-thaw stage. The results showed that egg yolk free commercial Optixcell extender and egg yolk based TFYG extender were at par in terms of most of the sperm quality traits, hence any one of them can be preferred over Andromed for successful routine cryopreservation of cattle and buffalo semen.

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A systematic review and meta-analysis of spermatozoa cryopreservation, in vitro and in vivo fertility practices in water buffalo

Shah

Andrabi²

2021

Vet Res Commun

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OPTIXcell improves the postthaw quality and fertility of buffalo bull sperm

Cited by 23 publications

References 36 publications

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Efficacy of egg yolk based and egg yolk free soya bean milk based extenders for cryopreservation of Zebu cattle and buffalo semen

A systematic review and meta-analysis of spermatozoa cryopreservation, in vitro and in vivo fertility practices in water buffalo

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