Optoacoustic mesoscopy for biomedicine

Omar, Murad; Aguirre, Juan; Ntziachristos, Vasilis

doi:10.1038/s41551-019-0377-4

Cited by 170 publications

(151 citation statements)

References 221 publications

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“…Photoacoustic imaging (PAI) is a rapidly growing biomedical imaging technique used in various clinical and preclinical applications. [1][2][3][4][5][6][7] PAI is based on photoacoustic (PA) effect, wherein ultrasound (US) signals are generated on absorption of light by an absorber. In PAI, the target samples or tissues generate US signals after being irradiated by laser pulses.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

et al. 2019

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“…Reflection-based imaging tends to provide primarily morphological information, while absorption-based imaging resolves the distributions of specific biomolecules and can even indicate concentrations in some cases. 22 The two most important mesoscopic imaging techniques, optical coherence tomography (OCT) and high-frequency ultrasonography (HFUS), 23 are reflection-based: OCT detects reflected light, while HFUS detects reflected ultrasound. 22 Optical coherence tomography is increasingly used in dermatological clinical practice.…”

Section: Mesoscopy Methodsmentioning

confidence: 99%

“…22 The two most important mesoscopic imaging techniques, optical coherence tomography (OCT) and high-frequency ultrasonography (HFUS), 23 are reflection-based: OCT detects reflected light, while HFUS detects reflected ultrasound. 22 Optical coherence tomography is increasingly used in dermatological clinical practice. In OCT, the skin tissue is illuminated 1,7,89 Multiphoton Microscopy (MPM) 1,25,90 Optical Coherence Tomography (OCT) 24,25,28,91,92 20-MHz High-frequency ultrasound (HFUS) 34,37,93 Raster-scan optoacoustic mesoscopy (RSOM) 33,59,94…”

Section: Mesoscopy Methodsmentioning

confidence: 99%

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Hindelang

Aguirre

Schwarz

et al. 2019

Acad Dermatol Venereol

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In recent years, several non‐invasive imaging methods have been introduced to facilitate diagnostics and therapy monitoring in dermatology. The microscopic imaging methods are restricted in their penetration depth, while the mesoscopic methods probe deeper but provide only morphological, not functional, information. ‘Raster‐scan optoacoustic mesoscopy’ ( RSOM ), an emerging new imaging technique, combines deep penetration with contrast based on light absorption, which provides morphological, molecular and functional information. Here, we compare the capabilities and limitations of currently available dermatological imaging methods and highlight the principles and unique abilities of RSOM . We illustrate the clinical potential of RSOM , in particular for non‐invasive diagnosis and monitoring of inflammatory and oncological skin diseases.

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“…For 25 years, PA imaging has been an active area and is now one of the hottest topics in biomedical optics. PA tomography (PAT) and microscopy (PAM) have numerous diagnostic applications [9][10][11], providing both endogenous [12][13][14][15] and exogenous molecular contrast in structural and functional images [15][16][17]. In particular, PA spectroscopy has produced functional images in the brain and heart [10,[18][19][20], and molecular images using the unique spectra of nanoengineered contrast agents [16,[20][21][22].…”

Section: Mainmentioning

confidence: 99%

Real-time spectroscopic photoacoustic/ultrasound (PAUS) scanning with simultaneous fluence compensation and motion correction for quantitative molecular imaging

Jeng

Kim

et al. 2019

Preprint

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For over two decades photoacoustic (PA) imaging has been tested clinically, but successful human trials have been minimal. To enable quantitative clinical spectroscopy, the fundamental issues of wavelength-dependent fluence variations and inter-wavelength motion must be overcome. Here we propose a new real-time, spectroscopic photoacoustic/ultrasound (PAUS) imaging approach using a compact, 1-kHz rate wavelength-tunable laser. Instead of illuminating tissue over a large area, the fiber-optic delivery system surrounding an US array sequentially scans a narrow laser beam, with partial PA image reconstruction for each laser pulse. The final image is then formed by coherently summing partial images at a 50-Hz video rate. This scheme enables (i) automatic laser-fluence compensation in spectroscopic PA imaging and (ii) interwavelength motion correction using US speckle tracking, which have never been shown before in real-time systems. The 50-Hz video rate PAUS system is demonstrated in vivo using a murine model of drug delivery monitoring. AffiliationsContributions G.-S. J. assembled the experimental setup, developed and implemented the PAUS imaging protocol, integrated the optical system with a Vantage (Verasonics) US scanner, performed all experimental studies, performed PA and US image processing and reconstruction, developed and implemented motion correction algorithms for spectroscopic PA imaging, wrote the paper. M.-L. L. conducted experiments with G.-S. J., helped in image processing. M.K. conducted experiments with G.-S. J., developed and implemented laser fluence compensation routine in spectroscopic PA imaging. S.J.Y. assembled early-stage PAUS system, did preliminary measurements, helped in designing imaging protocol for the final PAUS scanner. J.J.P. Jr. participated in designing laser fluence compensation algorithms, fast acquisition and image processing in Verasonics US scanner and article illustration. D.S.L. synthesized Prussian blue nanocubes, helped in auxiliary measurements. I.P. conceived the idea of the spectroscopic fast-sweep approach, designed the project with M.O.D., supervised the project, specified the laser and fiber-optic coupling modules for the PAUS system, worked with vendors for their development, assembled the PAUS system, worked with M.K. on laser fluence compensation algorithms, participated in experimental studies, designed and wrote the paper. M.O.D. conceived the idea of moving beam illumination with I.P, designed the project, and wrote the paper.

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Optoacoustic mesoscopy for biomedicine

Cited by 170 publications

References 221 publications

Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Real-time spectroscopic photoacoustic/ultrasound (PAUS) scanning with simultaneous fluence compensation and motion correction for quantitative molecular imaging

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