Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

Sharma, Arunima; Srishti, .; Periyasamy, Vijitha; Pramanik, Manojit

doi:10.1117/1.jbo.24.12.121904

Cited by 33 publications

(29 citation statements)

References 43 publications

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“…In the PA MAP with 532 nm excitation (Figure 3cⅱ), blood vessels are invisible and internal organs, including the liver, spleen, and cecum, are seen. Penetration depth gets deeper as the optical wavelength gets longer [35], and thus the spine, rib cage, and large arteries near the thigh and tail are clearly visible (Figure 3c ⅲ and ⅳ). Next, we acquired PA MAP images of the spine and rib cage after they were removed from the body (Figure 3d).…”

Section: Resultsmentioning

confidence: 99%

Non-Ionizing Label-Free Photoacoustic Imaging of Bones

Park

Lee

et al. 2020

IEEE Access

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X-ray based radiography, the main modality for diagnostic imaging of bone structures and fractures, provides sensitive images, but it inherently involves potentially harmful X-ray exposure. As nonionizing alternatives, various optical imaging methods have been explored. Here, we demonstrate nonionizing, label-free, multispectral photoacoustic (PA) imaging of bones in small animals in vivo, in situ, and ex vivo. Using near-infrared light excitation and acoustic detection, the spine and ribs were successfully visualized in high-resolution PA images. PA 3D volume images of the spine and ribs were clearly visualized together with blood vessels and several organs including the spleen, liver, and cecum, without using any exogenous contrast agent nor ionizing radiation. Quantification results of multispectral PA signals from blood vessels and bones were in good agreement with their absorption coefficients. Further, a rib fracture was photoacoustically imaged. Our results demonstrate PA imaging's potential as a non-ionizing and label-free technique for imaging bone tissues.

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Section: Resultsmentioning

confidence: 99%

Non-Ionizing Label-Free Photoacoustic Imaging of Bones

Park

Lee

et al. 2020

IEEE Access

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“…Numbers marked in Figures 5E‐H show the corresponding regions detected in Figures 5I‐L. Due to high absorbance of blood at 532 nm, superficial vasculature was clearly visible in the acquired PA images, however, it was difficult to clearly discern the embryonic structures owing to the limited penetration depth of 532 nm .…”

Section: Resultsmentioning

confidence: 99%

“…A previously developed circular scan PAT system was used for acquiring cross‐sectional images of chicken embryo . Schematic of the system is shown in Figure .…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, in biological tissues, there is relatively decreased scattering and absorption of light of near‐infrared (NIR) wavelengths (700–1200 nm). Therefore, greater imaging depth can be achieved with illumination in NIR region . To achieve both high blood contrast and good imaging depth, PAT was performed at wavelengths of 532 and 740 nm.…”

Section: Introductionmentioning

confidence: 99%

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High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

Sharma

Ishak

Swee‐Hin

et al. 2020

Journal of Biophotonics

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Chicken embryos have been proven to be an attractive vertebrate model for biomedical research. They have helped in making significant contributions for advancements in various fields like developmental biology, cancer research and cardiovascular studies. However, a noninvasive, label-free method of imaging live chicken embryo at high resolution still needs to be developed and optimized. In this work, we have shown the potential of photoacoustic tomography (PAT) for imaging live chicken embryos cultured in bioengineered eggshells. Laser pulses at wavelengths of 532 and 740 nm were used for attaining cross-sectional images of chicken embryos at different developmental stages. Cross-sections along different depths were imaged to gain knowledge of the relative depth of different vessels and organs. Due to high optical absorption of vasculature and embryonic eye, images with good optical contrast could be acquired using this method. We have thus reported a label-free method of performing cross-sectional imaging of chicken embryos at high resolution demonstrating the capacity of PAT as a promising tool for avian embryo imaging. K E Y W O R D S bioengineered eggshell, chicken embryo development, photoacoustic computed tomography, photoacoustic imaging, vasculature imaging

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“…Identification of tumors in MSOT images is facilitated by key biomarkers such as local increases in vessel density around the tumor region [8 , 9] , changes in oxygen saturation in the tumor microenvironment [10 , 11] or alterations in the local distribution of fat, collagen, and other intrinsic tissue chromophores [12] . The MSOT imaging depth is maximized for optical wavelengths around 1064 nm due to relatively low scattering and absorption of light by living tissues and the high energy of commonly available lasers at this wavelength [13] . However, optoacoustic (OA) imaging is generally incapable of accurate delineation of tumor shape and boundaries.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive multiparametric characterization of mammary tumors with transmission-reflection optoacoustic ultrasound

et al. 2020

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Highlights A multi-modal platform is introduced for simultaneous imaging of optical absorption, ultrasound reflectivity and speed of sound with improved resolution in whole living mice. In vivo studies of 4T1 breast cancer xenografts models revealed highly synergistic value of the hybrid imaging for characterizing mammary tumors. Multi-parametric characterization of tumor boundaries and neovascularization was demonstrated.

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Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation

Abstract: Photoacoustic imaging depth comparison at 532-, 800-, and 1064-nm wavelengths: Monte Carlo simulation and experimental validation," J.

Cited by 33 publications

References 43 publications

Non-Ionizing Label-Free Photoacoustic Imaging of Bones

Non-Ionizing Label-Free Photoacoustic Imaging of Bones

High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

Noninvasive multiparametric characterization of mammary tumors with transmission-reflection optoacoustic ultrasound

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