Oral administration of Bifidobacterium longum prevents gut-derived Pseudomonas aeruginosa sepsis in mice

Abstract

Aims: The aim of the study was to evaluate the efficacy of probiotics on gut‐derived sepsis caused by Pseudomonas aeruginosa in immunocompromised mice. Methods and Results: After oral inoculation of P. aeruginosa, mice were treated with cyclophosphamide to induce leucopenia and translocation of the intestinal P. aeruginosa into blood, thereby producing gut‐derived sepsis. In this model, administration of 1 × 109 CFU of Bifidobacterium longum strain BB536 for 10 days significantly (P < 0·01) increased the survival rate compared with groups of mice administered either with Bifidobacterium breve strain ATCC 15700 or excipients contained in the probiotic bacterial powder. Administration of B. longum significantly decreased viable counts of P. aeruginosa in the liver and blood compared with other groups. Culture of intestinal contents revealed a significantly lower viable count of P. aeruginosa in the jejunum of B. longum‐treated mice compared with other groups of mice. Furthermore, in vitro data demonstrated that B. longum possessed apparently higher adherent activity to Caco‐2 cell monolayers and significantly suppressed the adherence of P. aeruginosa to the monolayers of cells compared with other groups. Conclusion: Oral administration of B. longum protects mice against gut‐derived sepsis caused by P. aeruginosa, and the effect may be due to interference of P. aeruginosa adherence to intestinal epithelial cells. Significance and Impact of this Study: This study demonstrated that oral administration of B. longum BB536 is effective to protect against opportunistic infection with drug‐resistant bacteria such as P. aeruginosa. The results suggest that probiotics may play an important role even in the immunocompromised patients.

Oral probiotics: Lactobacillus sporogenes for prevention of necrotizing enterocolitis in very low-birth weight infants: a randomized, controlled trial

“…Although some of the studies (Lin et al, 2005;Matsumoto et al, 2008;Stoll and Hansen, 2008) predicated that probiotics may reduce the incidence of sepsis; literature did not confirm this association (Schanler, 2006;Deshpande et al, 2007). Our study did not show that L. sporogenes reduced the incidence of sepsis in VLBW infants.…”

Oral Administration of Heat-KilledLactobacillus plantarumStrain b240 Protected Mice againstSalmonella entericaSerovar Typhimurium

“…Several studies have reported that probiotic bacteria can adhere to intestinal epithelial cells and can competitively inhibit adhesion and invasion by enteric pathogens. 3,4,20,23) Moreover, heat-killed probiotic bacteria retain the ability to bind to cultured Int-407 and Caco-2 cells as well as viable probiotic bacteria.…”

Lactic acid bacteria activating innate immunity improve survival in bacterial infection model of silkworm

“…It was reported that Bifidobacterium protected E. coli O157 infection in germ-free mice (26). Oral administration of Bifidobacterium longum prevented P. aeruginosa gut-derived sepsis in a mouse model (27). Heat-killed L. casei protected against P. aeruginosa infection in mice (28).…”