Oral administration of crocetin prevents inner retinal damage induced by N-methyl-d-aspartate in mice

Ohno, Yuta; Nakanishi, Tomohiro; Umigai, Naofumi; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Hara, Hideaki

doi:10.1016/j.ejphar.2012.06.035

Cited by 66 publications

(47 citation statements)

References 23 publications

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“…Scientific studies have confirmed the validity of saffron extracts in the treatment of mild to moderate depression [5,6], and agent against erectile dysfunction [7], and as anti-arrhythmic agent and restorer of cardicac functions [8,9]. Other studies showed a role of saffron as a protector against age related macular degeneration [10,11], in reducing cognitive decline in patients with Alzheimer Disease [12,13], and as a source for potential anti-cancer agents [14,15,16].…”

Section: Introductionmentioning

confidence: 88%

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Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

et al. 2015

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Section: Introductionmentioning

confidence: 88%

“…Two hundred grams oven-dried flowers of saffron 10 were weighed on an analytical balance. Authentic standards of kaempferol and crocin were purchased from Sigma-Aldrich and used to confirm the identity of isolated compounds.…”

Section: Plant Materialsmentioning

confidence: 99%

Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

et al. 2015

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“…This inhibition was associated with reduced cell proliferation through the downregulation or upregulation of certain apoptotic proteins, including cytochrome c and cleaved caspase-3 expression. In addition, a number of pharmacological studies have demonstrated crocetin to have multiple activities, including anti-inflammatory (35,36) and anti-oxidant (37,38) effects. Therefore, it was hypothesized that the anti-oxidant and anti-inflammatory effects of crocetin may also be considered to be involved in several synergistic antitumor mechanisms (39,40).…”

Section: D a Bmentioning

confidence: 99%

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Wang

et al. 2013

Molecular Medicine Reports

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Abstract. Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin-sensitive treatment of GC and its possible mechanisms. BGC-823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC-823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase-3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose-and time-dependent manner. Apoptotic BGC-823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC-823 cells. Crocetin also induced caspase-3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC-823 cells, and may be used as an effective agent in the treatment of GC.

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“…However, the other mechanisms had been reported in partly. In previous reports, the protective effects of crocetin against various stresses in retina related to inhibition activity for caspase-3 and caspase-9 [14,15]. These results suggest that crocetin also inhibits caspase-3 and caspase-9 expression to protect the brain against cells apoptosis.…”

Section: Discussionmentioning

confidence: 93%

“…Crocetin has been reported to inhibit Aβ fibrillization and stabilize Aβ oligomers [13]. We recently reported that crocetin prevents retinal damage induced by H 2 O 2 , tunicamycin, and N-methyl-Daspartate (NMDA) [14,15]; saffron extracts have been reported to be effective on memory deficit on mild AD patient [16,17]. However, there was no report on protective effects of crocetin in hippocampal neuronal cells having been examined.…”

Section: Introductionmentioning

confidence: 97%

Crocetin Prevents Amyloid <i>β</i><sub>1-42</sub>-Induced Cell Death in Murine Hippocampal Cells

Yoshino¹,

Ishisaka²,

Umigai³

et al. 2014

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Crocetin is an aglycon of carotenoid extracted by saffron stigmas (Crocus sativus L.) and known to have a potent anti-oxidative effect. Amyliod β (Aβ), hallmark of Alzheimer's disease, is reported to have neurotoxicity partly via oxidative stress. In this study, we investigated the effect of crocetin on hippocampal HT22 cell death induced by Aβ 1-42 . Furthermore, to clarify the mechanism underlying the protective effects of crocetin against Aβ 1-42 -induced cell death, we measured reactive oxygen species (ROS) production by CM-H 2 DCFDA kit assay. Crocetin at 1 -10 μM protected HT22 cells against Aβ 1-42 -induced neuronal cell death and decreased ROS production increased by Aβ 1-42 . These results that crocetin has the potent neuroprotective effect against Aβ 1-42 -induced cytotoxicity in hippocampal cells by attenuating oxidative stress, suggest that crocetin may provide a useful therapeutic strategy against Aβ-related disorders.

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Oral administration of crocetin prevents inner retinal damage induced by N-methyl-d-aspartate in mice

Cited by 66 publications

References 23 publications

Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Crocetin Prevents Amyloid <i>β</i><sub>1-42</sub>-Induced Cell Death in Murine Hippocampal Cells

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Oral administration of crocetin prevents inner retinal damage induced by N-methyl-d-aspartate in mice

Cited by 66 publications

References 23 publications

Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

Petals of Crocus sativus L. as a potential source of the antioxidants crocin and kaempferol

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Crocetin Prevents Amyloid &lt;i&gt;β&lt;/i&gt;&lt;sub&gt;1-42&lt;/sub&gt;-Induced Cell Death in Murine Hippocampal Cells

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Crocetin Prevents Amyloid <i>β</i><sub>1-42</sub>-Induced Cell Death in Murine Hippocampal Cells