Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Bürger, Beatriz; Kühl, Carolina Meneguetti Cintra; Candreva, Thamiris; Cardoso, Renato da S.; Silva, Jéssica R.; Castelucci, Bianca Gazieri; Consonni, Sílvio Roberto; Fisk, Helena L.; Calder, Philip C.; Vinolo, Marco Aurélio Ramirez; Rodrigues, Hosana Gomes

doi:10.1038/s41598-019-45508-1

Cited by 26 publications

(25 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These changes, in turn, resulted in subsequent increases in IL-10 production long in post-CPIP injuries, like those observed in the current work at 15-day post-CPIP. Although we were unable to assess macrophage phenotypes at 15-day post-CPIP due to financial constraints, our findings are in agreement with Burger et al (2019) , who observed an acceleration in the healing process following EPA supplementation for 4 weeks, with these changes in parallel with an increase in M2 macrophages and IL-10 on the 7th day after the induction of skin wounds in mice. Based on these findings, we hypothesize that omega-3 supplementation might help shift the body toward a more anti-inflammatory phenotype following the injuries that are known to trigger CRPS-I.…”

Section: Discussionsupporting

confidence: 90%

Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice

Fernandes

Galassi

Horewicz

et al. 2022

Front. Integr. Neurosci.

View full text Add to dashboard Cite

ObjectiveComplex regional pain syndrome (CRPS) is usually triggered by trauma or a surgical procedure, and it typically becomes an established one after an intense inflammatory process with chronic pain and edema as the main symptoms. Available treatments for CRPS have low efficacy. This study aimed to evaluate the clinical and immunoregulatory effects of omega-3 polyunsaturated fatty acid (PUFA) supplementation on paw edema and anti- and pro-inflammatory cytokines and macrophage phenotypes in the chronic post-ischemia pain (CPIP) preclinical model of CRPS-Type I.MethodsFemale Swiss mice were supplemented with omega-3, corn oil, or saline and then submitted to the CPIP model of ischemia/reperfusion (I/R) injury. Supplementation was carried out for 30 days prior to and up to 2 or 15 days after the induction of CPIP, according to experimental protocols. The supplementation protocol included 1,500 mg/kg of omega-3 or corn oil through an intragastric route (gavage). Paw edema, interleukin- (IL-) 4, IL-10, transforming growth factor-β1 (TGF-β1), monocyte chemotactic protein-1 (MCP-1), and tumor necrosis factor (TNF) were then measured in the paw skin and muscle by enzyme-linked immunosorbent assay (ELISA), and macrophage phenotypes (M1 and M2) assessed in the paw muscle by Western blotting.ResultsThe CPIP model induced an increase in paw thickness up to 72 h post-I/R. Mice supplemented with omega-3 compared to the saline group displayed reduced edema but neither altered skin IL-4 or skin and muscle TGF-β1, TNF, and MCP-1 concentrations, nor did they exhibit significantly altered muscle macrophage phenotype on the 2nd-day post-CPIP. However, omega-3 supplementation reversed the I/R-related reduction in IL-4 in the paw muscle compared to groups supplemented with saline and corn oil. Furthermore, omega-3 promoted the reduction of IL-10 levels in the paw skin, compared to animals with lesions supplemented with saline, until the 2nd-day post-CPIP. On the 15th day post-CPIP, IL-10 was significantly increased in the muscle of animals supplemented with omega-3 compared to the saline group.ConclusionThe results suggest that omega-3 PUFA supplementation has anti-inflammatory effects in the CPIP model of CRPS-Type I, significantly reducing paw edema and regulating concentrations of anti-inflammatory cytokines, including IL-4 and IL-10.

show abstract

Section: Discussionsupporting

confidence: 90%

Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice

Fernandes

Galassi

Horewicz

et al. 2022

Front. Integr. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Several mouse studies of experimental periodontitis induced by ligature placement for up to 2 weeks reported short‐ to long‐term post‐ligation increases in levels of pro‐inflammatory cytokines such as IL‐1β and IL‐6 and a weak increasing trend in IL‐10 48–50 . Although few studies have analyzed these cytokines in ligature‐removal repair models, Burger et al 51 examined wound healing in mouse epithelial tissue. Their study showed that levels of IL‐1β and IL‐6 protein increased in surgically wounded tissue and then decreased 1 day after injury, whereas IL‐10 levels continued to increase until day 7.…”

Section: Discussionmentioning

confidence: 99%

Wound healing in periodontal disease induces macrophage polarization characterized by different arginine‐metabolizing enzymes

Miyashita

Kuraji

et al. 2021

J of Periodontal Research

View full text Add to dashboard Cite

Background and objective Macrophages play important roles from the initiation of inflammation to wound healing. Two phenotypes of macrophages, namely pro‐inflammatory type macrophages (M1‐MΦ) and anti‐inflammatory type macrophages (M2‐MΦ), have been reported. Two contrasting metabolic enzymes that use arginine as a substrate, inducible nitric oxide synthase (iNOS), and arginase‐1 (Arg‐1), have been identified as M1‐MΦ and M2‐MΦ markers, respectively. The purpose of this study was to elucidate the temporal dynamics of the macrophage phenotype during the progression and healing phases of experimental periodontitis in mice. Material and methods A total of 63 C57BL/6J mice were divided into the following 3 groups: control (C), periodontitis (P), and healing (H). To induce periodontitis, a silk ligature was placed around the maxillary bilateral second molars of mice in the periodontitis and healing groups. In the healing group, the ligature was removed 3 days after ligation to induce tissue healing. Maxillary tissue was collected on day 0 for the control group, days 1, 3, 5, and 7 for the periodontitis group (P1, P3, P5, and P7), and days 5 and 7 for the healing group (H5 and H7: 3 days with the ligation + 2 days or 4 days following ligature removal). The left side of the maxilla was subjected to bone structure analysis using micro‐computed tomography and gene expression analysis using polymerase chain reaction. On the right side, immunohistochemistry was performed to histopathologically evaluate the localization of macrophages by phenotype in the periodontal tissue. Results In the alveolar bone structure analysis, the linear distance of bone height increased significantly in the P5 and P7 groups, whereas bone volume fraction and bone mineral density decreased over time after ligature placement; in the healing group (H5 and H7), these parameters improved significantly compared with the periodontitis group (P5 and P7). Expression of genes encoding pro‐inflammatory cytokines and iNOS increased in the periodontitis group, and expression of anti‐inflammatory cytokine genes and Arg‐1 increased in the healing group. Furthermore, the iNOS/Arg‐1 expression ratio increased with ligation, whereas the ratio in the healing groups (H5 and H7) significantly decreased compared with the periodontitis groups (P5 and P7). Immunofluorescence staining revealed a significant increase in the number of iNOS‐positive macrophages in the periodontitis group and decrease in the healing group. In contrast, the number of Arg‐1‐positive macrophages decreased in the periodontitis group and increased in the healing group. Conclusion The results of the present study suggest that wound healing in periodontal disease induces macrophage polarization from M1‐MΦ to M2‐MΦ characterized by iNOS and Arg‐1.

show abstract

“…Inhibition of the cyclooxygenase pathway results in inhibited prostaglandins so that there is no monocyte activation mechanism to produce TNF-α and interleukin 1β, which can inhibit collagen synthesis. 9,23 The limitation in this study was the difficulty in ensuring that the whole 1ml of lemuru fish oil gel was fully absorbed in the sulcus of the periodontal ligament. Therefore, further research on the absorption capacity of lemuru oil is required, to ensure the effect of lemuru as a topical drug in the treatment of periodontitis.…”

Section: Discussionmentioning

confidence: 97%

Lemuru fish oil gel as host modulation therapy in periodontal ligaments induced with Porphyromonas gingivalis

et al. 2020

View full text Add to dashboard Cite

Background: Periodontitis affects approximately 20%–50% of the global population and is caused by gram-negative bacteria, such as Porphyromonas gingivalis (P. gingivalis). Host modulation therapy (HMT) is part of a periodontal therapy that is used as an adjunct to conventional periodontal treatment to reduce tissue damage. Lemuru fish oil containing EPA and DHA can reduce the formation of MMPs and will further increase the number of fibroblasts there by stimulating collagen formation. Purpose: To determine the effect of lemuru fish oil gel on the collagen density and width of the periodontal tissue induced by P. gingivalis and the correlation between these parameters. Methods: Thirty male Wistar rats were divided into five groups. Induction of P. gingivalis was carried out first, then lemuru fish oil gel was applied to the gingival sulcus for 14 days, according to collagen scores in histological preparations using Masson's trichrome (MT). The width of the periodontal ligament was measured with an image raster program in µm. The data were analysed using statistics to test hypotheses using SPSS version 24. Results: Significant differences in the results of the collagen density were observed between groups K- and K+ and groups K+ and P2. Meanwhile, no significant difference was observed between groups K- and P2, P3, P2 and P3 and K+ and P1. The mean values of the periodontal ligament widths were K- (299.61 ± 51.82µm), K+ (425.85 ± 61.54µm), P1 (346.93 ± 33.53µm), P2 (370.15 ± 49.42µm) and P3 (379.6 ± 49.26). Conclusion: Lemuru fish oil can affect the width of the ligament and the collagen density with an optimal concentration of 20%. The correlation between the collagen density and the periodontal ligament width was negative and not significant.

show abstract

Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Cited by 26 publications

References 61 publications

Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice

Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice

Wound healing in periodontal disease induces macrophage polarization characterized by different arginine‐metabolizing enzymes

Lemuru fish oil gel as host modulation therapy in periodontal ligaments induced with Porphyromonas gingivalis

Contact Info

Product

Resources

About