Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets

Li, Yinghui; ShuLing, Hou; Chen, Jiashun; Peng, Wei; Wen, Wei; Chen, Fengming; Huang, Xingguo

doi:10.1016/j.jff.2019.103591

Cited by 20 publications

(22 citation statements)

References 32 publications

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“…Lactobacillus species are one taxon of the resident bacteria in the gastrointestinal tract of most animals, which are usually used as probiotics ( 20 , 21 ). The results of the present study showed that dietary supplementation of Lactobacillus delbrueckii enhanced the ADG of the weaned piglets, which was consistent with the results of previous research that oral administration of Lactobacillus delhrueckii during the suckling period increased the ADG of pre-weaning piglets ( 11 ). One possible reason for the result could be the improvement in nutrient absorption.…”

Section: Discussionsupporting

confidence: 93%

“…The 2-cm intestinal tissue samples were stained with hematoxylin and eosin (HE) as described previously ( 11 ). Villus height (from the villi tip to the villus–crypt joint) and crypt depth (from the villus–crypt joint to the base of the crypt) were measured under an Olympus Van-Ox S microscope (Opelco, Washington, DC, USA) using an image analysis software (Image-Pro, Media Cybernetics, Inc., Silver Springs, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, oral administration of Lactobacillus delbrueckii to suckling piglets improved the immune response, intestinal morphology, barrier function, and growth ( 11 ). Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese, exerting beneficial probiotic roles ( 12 ).…”

Section: Introductionmentioning

confidence: 98%

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Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets

Wang

Liu²,

et al. 2021

Front. Vet. Sci.

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Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary level of 0.1% in diets for weaned piglets. Eighty healthy weaned piglets (initial body weight: 7.56 ± 0.2 kg) were randomly divided into two feeding groups with four replicates in each group (n = 10 animals per replicate); piglets were fed with basal diet (CON) or basal diet containing 0.1% Lactobacillus delbrueckii (LAC). The results showed that dietary supplementation of Lactobacillus delbrueckii improved growth performance and increased serum HDL and insulin levels in piglets on the 28th day of the experimental time (p < 0.05). The gut microbe analysis revealed that Lactobacillus delbrueckii significantly decreased the relative abundance of the phyla Bacteroidetes, but increased the relative abundance of the phyla Firmicutes. The Lactobacillus delbrueckii also significantly increased the relative abundance of Bifidobacterium and Lactobacillus at the genus level of the bacterial community in the ileum, but decreased the relative abundance of unclassified Clostridiales. Moreover, Lactobacillus delbrueckii improved mucosal morphology by obtaining higher intestinal villus height (p < 0.05), significantly increasing the concentrations of butyrate, isobutyric acid, and isovaleric acid in colonic chyme of piglets, but decreasing the intestinal pH at the duodenum and ileum on the 28th day of the experimental time. In conclusion, dietary supplementation of Lactobacillus delbrueckii in the diet of weaned piglets can improve intestinal morphology and modulate the microbiota community to promote growth performance.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

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Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets

Wang

Liu²,

et al. 2021

Front. Vet. Sci.

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“…These probiotics exert their effects through a variety of mechanisms, including stimulating the immune response, preventing the invasion of pathogens, and producing antibacterial substances [ 27 ]. Previously, our laboratory observed that average daily feed intake (ADG) and body weight at Day 21 were increased by the oral administration of LAB to preweaning piglets during the lactation stage [ 28 ]. Moreover, Lan et al [ 29 ] and Liu et al [ 30 ] reported that probiotic supplementation could improve the growth performance of piglets.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Chen

et al. 2021

Antioxidants

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Oxidative stress is increasingly being recognized as a player in the pathogenesis of intestinal pathologies, and probiotics are becoming an attractive means of addressing it. The present study investigated the effects of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal integrity and oxidative damage in lipopolysaccharide (LPS)-challenged piglets. A total of 36 crossbred weaned piglets (Duroc × Landrace × Large Yorkshire) were randomly divided into three groups: (1) non-challenged controls (CON), (2) LPS-challenged controls (LPS), and (3) 0.2% LAB (2.01 × 1010 CFU/g) + LPS treatment (LAB + LPS). On the 29th day of the experiment, the LPS and CON groups were injected intraperitoneally with LPS and saline at 100 ug/kg body weight, respectively. The results show that the LPS-induced elevation of the serum diamine oxidase (DAO) level and small intestinal crypt depth (CD) were reversed by the dietary addition of LAB, which also markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) in the LPS-challenged piglets. Furthermore, LAB supplementation normalized other LPS-induced changes, such as by decreasing malondialdehyde (MDA) in both the serum and intestinal mucosa and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the jejunal mucosa, increasing glutathione reductase (GR) and glutathione peroxidase (GSH-Px) in both the serum and intestinal mucosa, and increasing glutathione (GSH) and superoxide dismutase (SOD) in the jejunal mucosa. LAB also activated Toll-like receptor (TLR)–Bruton’s tyrosine kinase (Btk)–nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathways in the intestine, suggesting that it plays a vital role in the ameliorative antioxidant capacity of weaned piglets. In summary, LAB increased intestinal integrity by improving the intestinal structure and tight junctions while enhancing antioxidant functions via the activation of the TLR–Btk–Nrf2 signaling pathway.

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“…Functional analysis indicates that the selective paracellular permeability could be regulated by tight junctions, the multi-protein complexes located in the lateral space between individual cells (9). Tight junctions consist of a branching network of proteins, among which Occludin (10) and Claudin (11) are trans-membrane proteins and scaffolding ZO-1 anchors the cytoskeleton to the transmembrane domains of Occludin (12). All these integral proteins are suggested to be dynamic (13) and have pivotal roles in regulating barrier junction and even cell survival (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus salivarius, a Potential Probiotic to Improve the Health of LPS-Challenged Piglet Intestine by Alleviating Inflammation as Well as Oxidative Stress in a Dose-Dependent Manner During Weaning Transition

Sun

et al. 2020

Front. Vet. Sci.

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Intestinal health is a critical issue for piglets during their weaning transition period. Previous reports have emphasized the promise of distinct probiotics in improving the enteric health. Here in this research, a newly isolated Lactobacillus salivarius strain was pretreated to Lipopolysaccharide (LPS)-challenged piglets and its association with integrity of the intestinal barrier coupled with effective dosage were expected to be signified. In the present study, 72 piglets (Landrace × Yorkshiere × Duroc) were randomly allotted to four groups, each group with six replicates. The subjects in the control group were provided with basal diet while those in other tested groups with extra 0.05, 0.1, and 0.2% L. salivarius, respectively. Fourteen days later, LPS was intraperitoneally injected and sodium pentobarbital was then delivered to euthanize those LPS-challenged piglets. An increase of average daily gain and body weight along with an apparent decline of diarrhea rate were observed in L. salivarius-treated groups. Both 0.1 and 0.2% L. salivarius supplement in total diet had the capability to markedly elevate levels of CAT, GSH-Px, SOD, anti-inflammatory cytokine from the serum as well as tight junction proteins (Claudin-1, Occludin, and ZO-1) extracted from intestine in LPS-challenged piglets. These changes were accompanied by the obvious downregulation of D-lactic acid, DAO, MDA and pro-inflammatory mediators in the serum, including IL-1β, IL-6, IFN-γ, and TNF-α. Meanwhile, the expression levels of TLR2 and TLR4 in spleen and mesenteric lymph nodes were significantly lower whereas the oxidation-related gene, ho-1 was up-regulated with L. salivarius administration. Our findings suggested that relatively high dose L. salivarius (0.1–0.2%) could regulate the progression of inflammatory response and oxidative stress when individuals were exposed to LPS, thus probably offering valuable assistance in restoring barrier function and improving overall performance.

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Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets

Cited by 20 publications

References 32 publications

Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets

Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Lactobacillus salivarius, a Potential Probiotic to Improve the Health of LPS-Challenged Piglet Intestine by Alleviating Inflammation as Well as Oxidative Stress in a Dose-Dependent Manner During Weaning Transition

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