Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats

Rodrigues, Hosana Gomes; Vinolo, Marco Aurélio Ramirez; Sato, Fábio Takeo; Magdalon, Juliana; Kühl, Carolina Meneguetti Cintra; Yamagata, Ana S.; Pessoa, Ana Flávia Marçal; Malheiros, Gabriella; Santos, Marinilce Fagundes dos; Lima, Camila; Farsky, Sandra Helena Poliselli; Câmara, Niels Olsen Saraiva; Williner, María Rosa; Bernal, Claudio; Calder, Philip C.; Curi, Rui

doi:10.1371/journal.pone.0165115

Cited by 50 publications

(49 citation statements)

References 62 publications

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“…Linolenic acid and its derivatives have been shown to modify the immune response, inhibiting the inflammatory response through inhibition of pro-inflammatory cytokines such as tumour necrosis-α and interleukin-12; and also promoting wound healing [42]. Linolenic acid has also been shown to improves cell migration [43], enhance cerebral vasodilatation [44] and induce angiogenesis [45]. Xanthine was related to wound hydration and this corroborates with Méchin et al who proposed xanthine derivatives are able to correct the hydration of the epidermis [46].…”

Section: Discussionmentioning

confidence: 99%

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Ashrafi

Muhamadali

et al. 2020

PLoS ONE

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Profiling skin microbiome and metabolome has been utilised to gain further insight into wound healing processes. The aims of this multi-part temporal study in 11 volunteers were to analytically profile the dynamic wound tissue and headspace metabolome and sequence microbial communities in acute wound healing at days 0, 7, 14, 21 and 28, and to investigate their relationship to wound healing, using non-invasive quantitative devices. Metabolites were obtained using tissue extraction, sorbent and polydimethylsiloxane patches and analysed using GCMS. PCA of wound tissue metabolome clearly separated time points with 10 metabolites of 346 being involved in separation. Analysis of variance-simultaneous component analysis identified a statistical difference between the wound headspace metabolome, sites (P = 0.0024) and time points (P<0.0001), with 10 out of the 129 metabolites measured involved with this separation between sites and time points. A reciprocal relationship between Staphylococcus spp. and Propionibacterium spp. was observed at day 21 (P<0.05) with a statistical correlation between collagen and Propionibacterium (r = 0.417; P = 0.038) and Staphylococcus (r = -0.434; P = 0.03). Procrustes analysis showed a statistically significant similarity between wound headspace and tissue metabolome with non-invasive wound devices. This exploratory study demonstrates the temporal and dynamic nature of acute wound metabolome and microbiome presenting a novel class of biomarkers that correspond to wound healing, with further confirmatory studies now necessary.

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Section: Discussionmentioning

confidence: 99%

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Ashrafi

Muhamadali

et al. 2020

PLoS ONE

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“…LA constitutes 40% of the fatty acids in the skin and plays an important role for its function [21]. In previously published studies from our group, a beneficial function of ω-6 in the skin wound healing process has been demonstrated [19,21].…”

Section: Discussionmentioning

confidence: 91%

“…After homogenization, samples were centrifuged, and the supernatant collected. IL-1β, IL-6, IL-10, TNF-α, CXCL-1, CXCL-2, TIMP-1 and VEGF concentrations were determined by ELISA as previously described [21] using the Duo Set kit (R&D System, Minneapolis, MN, U.S.A.). The results were normalized to the amount of protein in the tissue homogenate determined using the method of Bradford [22].…”

Section: Cytokines Chemokines and Growth Factors In Skin Tissuementioning

confidence: 99%

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Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue

et al. 2019

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There is no consensus on the effects of omega-3 (ω-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous ω-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) ω-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-α concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-α and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-α content and CD45+F4/80+ cells at the third day after skin wounding and increased concentrations of important metabolites derived from ω-3, like 18-HEPE, and reduced concentrations of those from ω-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue.

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“…However, on the 14th and 21st day of healing, the number of new formed vessels was not different in relation to the other groups. Angiogenesis has also been observed in wounds of diabetic rats with the administration of brown algae, Cystoseira trinodis 15 , Curcumim 16 and linoleic acid 17 , but these compounds showed angiogenic activity for more than 14 days.…”

Section: ■ Discussionmentioning

confidence: 99%

Effect of powdered shells treatment of the snail Megalobulimus lopesi on wounds of diabetic rats

Andrade

Portugal²,

Rondon³

et al. 2018

Acta Cir. Bras.

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Purpose: To analyzed the healing effect of the powdered shell of the Megalobulimus lopesi snail on wounds of diabetic rats, since in non-diabetic rats the powdered shell presented healing potential. Methods: Seventy-two Wistar rats (Rattus norvegicus albinus) were divided into three groups: Control group (GC.diab), no therapeutic intervention on the wound; Vehicle's Control group, topical via, in diabetic rats (GCvt.diab): Powder Shell Group (PC) applied topically (GPCvt.diab): Experimental group was administered topically shortly after wound dressing and once a day during the experimental period (3, 7, 14 and 21 days) the composition containing the powdered shell of the snail. The following variables related to the healing potential were analyzed: macroscopic one, where the capacity of reduction of the wound area was evaluated; histological analysis in HE, angiogenic activity, morphometric analysis (re-epithelization), leukocyte inflammatory infiltrate; leukocyte count and also differentiation in peripheral blood. Results: The topical application in wounds of diabetic rats presented healing activity, accelerating wound closure, stimulating angiogenesis and being pro-inflammatory in the early and anti-inflammatory stages in the final times of the healing process. Conclusion:The topical administration of the powdered shell on wounds of diabetic patients becomes a therapeutic option of low cost, with ease in the administration and access as well.

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Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats

Cited by 50 publications

References 62 publications

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue

Effect of powdered shells treatment of the snail Megalobulimus lopesi on wounds of diabetic rats

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