Oral administration of pulverized Konjac glucomannan prevents the increase of plasma immunoglobulin E and immunoglobulin G levels induced by the injection of syngeneic keratinocyte extracts in BALB/c mice

Abstract

These results suggested that oral administration of PKGM prevents the elevation of plasma IgE by suppressing IgE class switching in B cells and/or the commitment development of naive lymphocytes to both T-helper type 1 (Th1) and Th2.

Dietary Pulverized Konjac Glucomannan Prevents the Development of Allergic Rhinitis-Like Symptoms and IgE Response in Mice

“…This result differs from our previous data, which indicated that dietary PKGM suppressed both total IgE and IgG1 levels in NC/Nga mice 4,6) and in BALB/c mice upon injection with syngeneic keratinocyte extract. 7) This discrepancy in the data might be due to differences in experimental settings (e.g., mouse strain, immunization protocols).…”

“…7) Briefly, 96-well microtiter plates were coated with OVA solution (100 mg/ml) at 4 C overnight, and then incubated with diluted plasma samples ( 10 for IgE, and  500 for IgG1 and IgG2a). After reaction with secondary biotinylated rat anti-mouse IgE, IgG1, or IgG2a antibody (BD Bioscience, San Jose, CA, 1:250 dilution) and subsequent incubation with streptavidin-conjugated alkaline phosphatase (Zymed Laboratories, San Francisco, CA, 1:1,000 dilution), enzyme reaction was performed using the AttoPhos Ò substrate system (Promega Corporation, Madison, MI).…”

“…We have also reported that oral intake of PKGM inhibited the increase in serum IgE and IgG levels induced by continuous injection of syngeneic keratinocyte extracts in BALB/c mice. 7) Taken together, these results suggest that dietary PKGM can be beneficial in preventing allergy, although the precise mechanism underlying the anti-inflammatory action of PKGM and its effect on allergen-induced inflammatory disorders remain to be investigated.…”

Natural Products and Dermatological Hypersensitivity Diseases

“…Since the compound suppressed both Th1 and Th2 related cytokines it's mechanism of action cannot be attributed to Th1/Th2 polarization (Onishi et al, 2004(Onishi et al, , 2005. Follow up studies found that it decreased scratching behvaiour in a dose dependent manner (OnishiKawamotoSuzuki et al, 2007), and prevented IgE class switching in Balb/c mice following injection of keratinocyte extract (Oomizu et al, 2006). It was also found to suppress allergic rhinitis like inflammation following nasal challenge with ovalabumin (OnishiKawamotoUeda et al, 2007).…”

Vitamin E Improves Biochemical Indices Associated with Symptoms of Atopic Dermatitis-Like Inflammation in NC/Nga Mice

“…Furthermore, PGE2 production activates Th2 cells to secrete inflammatory cytokines, such as IL-5, which stimulate B-cells to induce IgE. It is recognized that high levels of IgE are one of the charac-teristics of AD, and clinical observations suggest a correlation between serum IgE levels and the extent of disease (18)(19)(20). Therefore, we assessed changes in COX-2 and IgE levels associated with the symptomatic cycle of AD in this study.…”