Oral administration of recombinant epinecidin-1 protected grouper (Epinephelus coioides) and zebrafish (Danio rerio) from Vibrio vulnificus infection and enhanced immune-related gene expressions

Pan, Chieh Yu; Huang, Tsui-Chin; Wang, Yi Da; Yeh, Yu Chih; Hui, Cho-Fat; Chen, Jyh-Yih

doi:10.1016/j.fsi.2012.01.023

Cited by 64 publications

(24 citation statements)

References 41 publications

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“…Septic shock was induced by intraperitoneal administration of V . vulnificus using experimental methods that have been described in several animal models [29,30]. The percent survival after injection of only TP3 at 20, 30, 40, 50 μg/fish was 86.7%, 73.3%, 66.7%, and 56.7%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Study of the Antimicrobial Activity of Tilapia Piscidin 3 (TP3) and TP4 and Their Effects on Immune Functions in Hybrid Tilapia (Oreochromis spp.)

Pan

Tsai

et al. 2017

PLoS ONE

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To address the growing concern over antibiotic-resistant microbial infections in aquatic animals, we tested several promising alternative agents that have emerged as new drug candidates. Specifically, the tilapia piscidins are a group of peptides that possess antimicrobial, wound-healing, and antitumor functions. In this study, we focused on tilapia piscidin 3 (TP3) and TP4, which are peptides derived from Oreochromis niloticus, and investigated their inhibition of acute bacterial infections by infecting hybrid tilapia (Oreochromis spp.) with Vibrio vulnificus and evaluating the protective effects of pre-treating, co-treating, and post-treating fish with TP3 and TP4. In vivo experiments showed that co-treatment with V. vulnificus and TP3 (20 μg/fish) or TP4 (20 μg/fish) achieved 95.3% and 88.9% survival rates, respectively, after seven days. When we co-injected TP3 or TP4 and V. vulnificus into tilapia and then re-challenged the fish with V. vulnificus after 28 days, the tilapia exhibited survival rates of 35.6% and 42.2%, respectively. Pre-treatment with TP3 (30 μg/fish) or TP4 (20 μg/fish) for 30 minutes prior to V. vulnificus infection resulted in high survival rates of 28.9% and 37.8%, respectively, while post-treatment with TP3 (20 μg/fish or 30 μg/fish) or TP4 (20 μg/fish) 30 minutes after V. vulnificus infection yielded high survival rates of 33.3% and 48.9%. In summary, pre-treating, co-treating, and post-treating fish with TP3 or TP4 all effectively decreased the number of V. vulnificus bacteria and promoted significantly lower mortality rates in tilapia. The minimum inhibitory concentrations (MICs) of TP3 and TP4 that were effective for treating fish infected with V. vulnificus were 7.8 and 62.5 μg/ml, respectively, whereas the MICs of kanamycin and ampicillin were 31.2 and 3.91 μg/ml. The antimicrobial activity of these peptides was confirmed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), both of which showed that V. vulnificus disrupted the outer membranes of cells, resulting in the loss of cell shape and integrity. We examined whether TP3 and TP4 increased the membrane permeability of V. vulnificus by measuring the fluorescence resulting from the uptake of 1-N-phenyl-naphthylamine (NPN). Treating fish with TP3 and TP4 under different pH and temperature conditions did not significantly increase MIC values, suggesting that temperature and the acid-base environment do not affect AMP function. In addition, the qPCR results showed that TP3 and TP4 influence the expression of immune-responsive genes, including interleukin (IL)-1β, IL-6, and IL-8. In this study, we demonstrate that TP3 and TP4 show potential for development as drugs to combat fish bacterial infections in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Study of the Antimicrobial Activity of Tilapia Piscidin 3 (TP3) and TP4 and Their Effects on Immune Functions in Hybrid Tilapia (Oreochromis spp.)

Pan

Tsai

et al. 2017

PLoS ONE

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“…In fish, they are able to modulate the expression of pro-inflammatory and other immune-related genes like IL-1β, IL-10, IL-22, IL-26, TNF-α, IFN-γ, NF-κB, lysozyme, NOS2, MyD88, TLR4a, TLR1, TLR3, [121,122,123,124,125]. Moreover, in mice this immunomodulatory effect also has been observed, with the modulation of the genes encoding IL-6, IL-10, IL-12, MCP-1, TNF-α, IFN-γ and IgG1 [126,127,128].…”

Section: Piscidinsmentioning

confidence: 99%

“…For example, epinecidin-1, when administrated orally or injected (in pre-, co- and post-infection) can significantly enhance survival in zebrafish and grouper that were challenged with Vibrio vulnificus [58,123,124]. Related to this, electrotransfer of epinecidin-1 in zebrafish and grouper muscle showed significant reduction in V. vulnificus and Streptococcus agalactiae bacterial counts [121,122,125].…”

Section: Therapeuticsmentioning

confidence: 99%

Antimicrobial Peptides from Fish

2014

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Antimicrobial peptides (AMPs) are found widely distributed through Nature, and participate in the innate host defense of each species. Fish are a great source of these peptides, as they express all of the major classes of AMPs, including defensins, cathelicidins, hepcidins, histone-derived peptides, and a fish-specific class of the cecropin family, called piscidins. As with other species, the fish peptides exhibit broad-spectrum antimicrobial activity, killing both fish and human pathogens. They are also immunomodulatory, and their genes are highly responsive to microbes and innate immuno-stimulatory molecules. Recent research has demonstrated that some of the unique properties of fish peptides, including their ability to act even in very high salt concentrations, make them good potential targets for development as therapeutic antimicrobials. Further, the stimulation of their gene expression by exogenous factors could be useful in preventing pathogenic microbes in aquaculture.

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“…Earlier studies showed that electroporation of epinecidin-1 plasmid DNA directly into zebrafish muscle induced antimicrobial activity, and oral administration of recombinant epinecidin-1 protected grouper (E. coioides) and zebrafish from V. vulnificus infection, as well as enhancing expression of immune-related genes [20,30]. These data indicate that epinecidin-1 is an excellent antimicrobial agent for application in aquaculture.…”

Section: Discussionmentioning

confidence: 83%

Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection

Jheng¹,

Lee²,

Ting³

et al. 2015

Fish & Shellfish Immunology

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Oral administration of recombinant epinecidin-1 protected grouper (Epinephelus coioides) and zebrafish (Danio rerio) from Vibrio vulnificus infection and enhanced immune-related gene expressions

Cited by 64 publications

References 41 publications

Study of the Antimicrobial Activity of Tilapia Piscidin 3 (TP3) and TP4 and Their Effects on Immune Functions in Hybrid Tilapia (Oreochromis spp.)

Study of the Antimicrobial Activity of Tilapia Piscidin 3 (TP3) and TP4 and Their Effects on Immune Functions in Hybrid Tilapia (Oreochromis spp.)

Antimicrobial Peptides from Fish

Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection

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