Oral administration of taurine improves experimental pancreatic fibrosis

Shirahige, Akinori; Mizushima, Takaaki; Matsushita, Koki; Sawa, Kiminari; Ochi, Koji; Ichimura, Mitsuko; Tanioka, Hiroaki; Shinji, Toshiyuki; Koide, Naoki; Tanimoto, Mitsune

doi:10.1111/j.1440-1746.2007.05127.x

Cited by 10 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent study, Masamune et al (2006) have demonstrated that curcumin, the yellow pigment in curry, inhibits PSC proliferation. Furthermore, other groups provided evidence that compounds like statins, taurine, or the green tea extract epigallocatechin gallate could also block the proliferation of PSCs in experimental in vitro or in vivo models, but the exact cellular mechanisms are still not fully understood (Jaster et al, 2003;Asaumi et al, 2006;Shirahige et al, 2007). Note that all of these mentioned substances are potent inducers of the cytoprotective enzyme HO-1 in several cell types, but no information is available concerning their effects on HO-1 in the pancreas (Olszanecki and Marcinkiewicz, 2004;Wu et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Schwer¹,

Guerrero²,

Humar³

et al. 2008

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Activation of pancreatic stellate cells (PSCs) is the key process in the development of pancreatic fibrosis, a common feature of chronic pancreatitis and pancreatic cancer. In recent studies, curcumin has been shown to inhibit PSC proliferation via an extracellular signal-regulated kinase (ERK)1/2-dependent mechanism. In addition, curcumin is a potent inducer of the cytoprotective enzyme heme oxygenase-1 (HO-1) in other cell types. Therefore, the aims of this study were to 1) characterize the effect of curcumin on HO-1 gene expression in PSCs, 2) explore whether HO-1 induction contributes to the inhibitory effect of curcumin on PSC proliferation, and 3) clarify the involvement of the mitogen-activated protein kinase (MAPK) family in this context. Cultured rat PSCs were incubated with curcumin and assessed for HO-1 up-regulation by Northern blot analysis, immunoblotting, and activity assays. The effect of HO-1 on platelet-derived growth factor (PDGF)-induced PSC proliferation and MAPK activation was determined by immunoblotting, cell proliferation assays, and cell count analyses. Curcumin induced HO-1 gene expression in PSCs in a timeand dose-dependent manner and inhibited PDGF-mediated ERK1/2 phosphorylation and PSC proliferation. These effects were blocked by treatment of PSCs with tin protoporphyrin IX, an HO inhibitor, or transfection of HO-1 small interfering RNA. Our data provide evidence that HO-1 induction contributes to the inhibitory effect of curcumin on PSC proliferation. Therefore, therapeutic up-regulation of HO-1 could represent a mode for inhibition of PSC proliferation and thus may provide a novel strategy in the prevention of pancreatic fibrosis.Pancreatic fibrosis is a common histopathological feature in chronic pancreatitis and pancreatic cancer. It is now generally accepted that pancreatic stellate cells (PSCs) play a crucial role in the fibrogenic process (Omary et al., 2007). In response to profibrogenic stimuli, PSCs undergo transdifferentiation from quiescent phenotypes into highly proliferative myofibroblast-like cells, which express the cytoskeletal protein ␣-smooth muscle actin and synthesize increased amounts of extracellular matrix components (Apte et al., 1998;Bachem et al., 1998). Recent studies have identified platelet-derived growth factor (PDGF)-BB as the most potent mitogen for pancreatic stellate cells in culture, and its effects have been extensively studied (Apte et al., 1998;Luttenberger et al., 2000). PDGF stimulates PSC cell proliferation by activating key effectors, including ras, raf, and the extracellular signal-regulated kinase (ERK)1/2 cascade (Jaster et al., 2002;Masamune et al., 2003a). Data from recent studies suggest that p38 mitogenactivated protein kinase (MAPK) and c-Jun NH 2 -terminal kinase (JNK) signaling pathways might also play a role in PSC proliferation ((Masamune et al., 2003b((Masamune et al., , 2004. Thus, modulation of MAPK activation is considered a potential strategy to inhibit PSC growth.Heme oxygenases (HOs) are the rate-limiting enzymes...

show abstract

Section: Discussionmentioning

confidence: 99%

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Schwer¹,

Guerrero²,

Humar³

et al. 2008

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

Taurine: A Potential Novel Addition to the Anti-Systemic Sclerosis Weaponry

Fallahzadeh

Namazi

Gupta

2010

Archives of Medical Research

View full text Add to dashboard Cite

High-resolution magic angle spinning¹H magnetic resonance spectroscopy detects choline as a biomarker in a swine obstructive chronic pancreatitis model at an early stage

et al. 2014

View full text Add to dashboard Cite

Chronic pancreatitis (CP) is a progressive inflammatory and fibrotic disease of the pancreas which encompasses a variety of clinical syndromes ranging from mild to life-threatening complications. Metabolomics has increasingly been applied to identify biomarkers for disease diagnosis with particular interest in diseases at an early stage. In this study, we tested a swine obstructive CP model by subtotal ligation of the main pancreatic duct, and the metabolic profiles of the Bama miniature swine pancreas were investigated using high-resolution magic angle spinning proton magnetic resonance spectroscopy (HR MAS (1)H MRS) combined with principal components analysis (PCA). Increases in lactate and choline for mild CP and decreases in glycerophosphocholine, phosphocholine, betaine and glycine were observed from normal pancreas to mild, moderate and severe CP. PCA results showed visual separations among the groups. The increase of choline at an early stage of CP and the decrease of glycerophosphocholine, phosphocholine, betaine and glycine reveal the pathogenesis of CP at a molecular level. The MRS results presented here demonstrate the potential of metabolic profiles in discriminating a normal pancreas from different stages of CP, which may be used to achieve CP early diagnosis and timely intervention to prevent irreversible destruction of the pancreas.

show abstract

Oral administration of taurine improves experimental pancreatic fibrosis

Abstract: The oral administration of taurine improves pancreatic fibrosis. Taurine chloramine inhibits transforming growth factor-beta1 produced from activated pancreatic stellate cells and improves pancreatic fibrosis.

Cited by 10 publications

References 31 publications

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Taurine: A Potential Novel Addition to the Anti-Systemic Sclerosis Weaponry

High-resolution magic angle spinning¹H magnetic resonance spectroscopy detects choline as a biomarker in a swine obstructive chronic pancreatitis model at an early stage

Contact Info

Product

Resources

About

Oral administration of taurine improves experimental pancreatic fibrosis

Abstract: The oral administration of taurine improves pancreatic fibrosis. Taurine chloramine inhibits transforming growth factor-beta1 produced from activated pancreatic stellate cells and improves pancreatic fibrosis.

Cited by 10 publications

References 31 publications

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway

Taurine: A Potential Novel Addition to the Anti-Systemic Sclerosis Weaponry

High-resolution magic angle spinning1H magnetic resonance spectroscopy detects choline as a biomarker in a swine obstructive chronic pancreatitis model at an early stage

Contact Info

Product

Resources

About

High-resolution magic angle spinning¹H magnetic resonance spectroscopy detects choline as a biomarker in a swine obstructive chronic pancreatitis model at an early stage