Oral and Nasal Sensitization Promote Distinct Immune Responses and Lung Reactivity in a Mouse Model of Peanut Allergy

Fischer, Romy; McGhee, Jerry R.; Vu, Huong; Atkinson, T. Prescott; Jackson, Raymond J.; Tomé, Daniel; Boyaka, Prosper N.

doi:10.1016/s0002-9440(10)61246-1

Cited by 51 publications

(55 citation statements)

References 79 publications

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“…We recently showed that mice challenged intranasally with peanut antigen developed eosinophil-mediated or neutrophil-mediated asthma depending of the initial nasal or oral route of sensitization [11]. Consistent with previous reports that both Th1 and Th2 cytokines are present in the lungs of asthmatic patients [12], Th1 and Th2 responses were not exclusive in orally sensitized (OS) and nasally sensitized (NS) mice.…”

Section: Introductionsupporting

confidence: 84%

“…Plasma levels of peanut-specific Abs were measured by ELISA as previously reported [11]. To improve the detection of IgE Abs [21], IgG were removed from plasma samples by overnight incubation of samples at 4°C on protein G coated 96-well plates (Reacti-Bind plates, Pierce, Rockford, IL).…”

Section: Plasma Antibody Responsesmentioning

confidence: 99%

“…To evaluate the presence of eosinophils, lung sections were incubated for 1 min in 10 mM KCN pH 6.5 before a 15 min incubation with the peroxidase substrate 3,3′-diaminobenzidine and counterstaining with hematoxylin [11].…”

Section: Histology and Determination Of Lung Inflammation Scoresmentioning

confidence: 99%

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Th1 and Th2 cells are required for both eosinophil- and neutrophil-associated airway inflammatory responses in mice

Fischer

Tomé

McGhee

et al. 2007

Biochemical and Biophysical Research Communications

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Most current animal models focus on eosinophil-mediated asthma, despite compelling evidence that a neutrophil-mediated disease occurs in some asthma patients. Using intranasal challenge of mice sensitized either orally or nasally with whole peanut protein extract in the presence of cholera toxin, we developed mouse models of eosinophil-and neutrophil-mediated asthma, respectively. In this study, mice deficient in Th1 (IL-12 and IFN-γ) or Th2 (IL-4 and IL-13) pathways were used to characterized the role played by Th1 and Th2 cytokines during the initial priming phase in the two models.. Antigen-specific Ab responses were controlled primarily by Th2 cytokines in mice sensitized by the oral route, whereas Th1 cytokines appeared to play a predominant role in mice sensitized by the nasal route. Furthermore, the absence of key Th1 or Th2 cytokines during the initial phase of priming reduced lung reactivity in both mouse models of airway inflammation.

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Section: Introductionsupporting

confidence: 84%

Section: Plasma Antibody Responsesmentioning

confidence: 99%

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Th1 and Th2 cells are required for both eosinophil- and neutrophil-associated airway inflammatory responses in mice

Fischer

Tomé

McGhee

et al. 2007

Biochemical and Biophysical Research Communications

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“…The recent development of murine models of peanut-induced anaphylaxis (PIA) that mimic physiologic and immunologic features of human PA has facilitated both understanding of the underlying mechanisms and evaluation of potential therapeutic approaches (11)(12)(13). An important focus of these models has been on the role of Th2 responses in the regulation of sensitization (14 -16) and, subsequently, therapeutic strategies to redirect immune responses away from Th2 (17,18).…”

mentioning

confidence: 99%

Impact of CD40 Ligand, B Cells, and Mast Cells in Peanut-Induced Anaphylactic Responses

Sun

Arias

Álvarez

et al. 2007

The Journal of Immunology

106

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The effector immune mechanisms underlying peanut-induced anaphylaxis remain to be fully elucidated. We investigated the relative contribution of Igs, mast cells (MCs), and FcεRI in the elicitation of anaphylaxis in a murine model. Assessment of peanut hypersensitivity reactions was performed clinically and biologically. Our data show that wild-type (WT; C57BL/6 strain) mice consistently developed severe anaphylaxis (median clinical score: 3.5/5), an ∼8°C drop in core body temperature, and significantly increased plasma levels of histamine and leukotrienes. CD40 ligand- and B cell-deficient mice presented evidence of allergic sensitization as demonstrated by production of Th2-associated cytokines by splenocytes and a late-phase inflammatory response that were both indistinguishable to those detected in WT mice. However, CD40 ligand- and B cell-deficient mice did not exhibit any evidence of anaphylaxis. Our data also show that MC-deficient (KitW/KitW-v) mice did not suffer, unlike their littermate controls, anaphylactic reactions despite the fact that serum levels of peanut-specific Igs were similarly elevated. Finally, FcεRI-deficient mice experienced anaphylactic responses although to a significantly lesser degree than those observed in WT mice. Thus, these data demonstrate that the presence of peanut-specific Abs along with functional MCs comprise a necessary and sufficient condition for the elicitation of peanut-induced anaphylaxis. That the absence of FcεRI prevented the development of anaphylaxis only partially insinuates the contribution of an IgE-independent pathway, and suggests that strategies to impair MC degranulation may be necessary to improve the efficacy of anti-IgE therapy.

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“…For 17 consecutive days the T I-T III groups were treated with ethanol extract of D. alata correspond to with their doses, T IV group was treated with antihistamine drugs and T V group was treated with diosgenin. On day 15, the NC and T I -T V groups were induced with ovalbumin of 0,0483 mg/mice (Fischer et al, 2005modified by Diding et al, 2008. Mice were sacrificed on day 18, the lymphocytes were isolated from spleen and the absolute number of lymphocytes from pellet suspension of lymphocyte was counted with haemocytometer 3 times on minor chamber.…”

Section: Methodsmentioning

confidence: 99%

LYMPHOCYTE PROLIFERATION ON HYPERSENSITIVITY OF Balb/C MICE AFTER GIVEN ETHANOL EXTRACT TUBER OF Dioscorea alata L.

Makiyah¹,

Djati²,

Rifa’i

et al. 2015

KLS

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Purple yam (Dioscorea alata L.) is a source nutritious tubers that has not been used optimally. Nutrients content in Dioscorea species are starch, essentials amino acid, minerals, polyphenol, glycoprotein, purin derivates such as allantoin, and steroid saponin which have biological activity such as immunomodulatory and antiallergic. The purpose of this research was to assess the absolute number of lymphocyte on hypersensitivity of mice after treated with ethanol extract of D. alata. Twenty one male Balb/C mice were used which were divided into seven groups: control (C), negative control (NC), treatments with ethanol extract of D. alata 0.17 g/kg, 2.01 g/kg, 10.04 g/kg (T I -T III), treatment with antihistamine drug 0,4 mg/mice/day (T IV) and treatment with diosgenin 200 mg/kg (T V). For 17 consecutive days the T I -T III groups were treated with ethanol extract of D. alata correspond to their doses, T IV group were treated with antihistamine drugs, and T V group were treated with diosgenin. On day 15, NC and T I -T V groups of mice were induced by ovalbumin 0,0483 mg/mice. Mice were sacrificed on day 18, and the lymphocyte was isolated from spleen, and the absolute number of lymphocyte was counted with Haemocytometer. Results showed that the absolute number of lymphocyte on mice hypersensitivity after treated with ethanol extract of D. alata L. were the lowest, while the highest absolute number of lymphocyte was found in the group treated with antihistamine drugs followed by the group treated with diosgenin on 200 mg/kg BW, negative control group and control group, respectively. Keywords: Balb/C mice, Dioscorea alata, ovalbumin, hypersensitivity, lymphocyte INTRODUCTIONPurple yam (Dioscorea alata L.) is a source of nutritious tubers that has not been used optimally. Nutrients content in Dioscorea species are starch, essentials amino Acid, mineral, polyphenol, mucilage (glycoprotein), purin derivates (such as allantoin) and steroid saponin (Wanasundera & Ravindran, 1994;Lape & Treche, 1994; Agbor-Egbe & Treche, 1995;Hikino et al.,1986, He et al., 1994Hu et al., 1996;Hu et al.,1997;Yang et al., 2003;Wang et al., 2011;Yoon et al., 2008). D. alata L. contains diosgenin (Cheng et al., 2007), a main steroidal saponin steroid aglicon as intermediate steroidal in pharmaceutical manufacture. Steroidal saponin is the most important bioactive compound due to its biological functions such as anticarcinogenic, antithrombotic, antiviral, hemolitic, hypocholesterolemic, hypoglichemic, immunostimulatory, antitumorogenic, antimutagenic, immunomodulatory and antiinflammatory (He et al., 1994;Hu et al., 1996;Hu et al., 1997;Yang et al.,2003). Results of previous studies showed that steroidal saponin has antiallergic activity (Zhang et al., 2012). The purpose of the research was to assess the absolute number of lymphocyte on hypersensitivity of mice after treated with ethanol extract of Dioscorea alata L. tubers.

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Oral and Nasal Sensitization Promote Distinct Immune Responses and Lung Reactivity in a Mouse Model of Peanut Allergy

Cited by 51 publications

References 79 publications

Th1 and Th2 cells are required for both eosinophil- and neutrophil-associated airway inflammatory responses in mice

Th1 and Th2 cells are required for both eosinophil- and neutrophil-associated airway inflammatory responses in mice

Impact of CD40 Ligand, B Cells, and Mast Cells in Peanut-Induced Anaphylactic Responses

LYMPHOCYTE PROLIFERATION ON HYPERSENSITIVITY OF Balb/C MICE AFTER GIVEN ETHANOL EXTRACT TUBER OF Dioscorea alata L.

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