Oral bacteria adherence to suture threads: an in vitro study

Neto, Oswaldo de Castro Costa; Lobo, Leandro Araújo; Iório, Natalia Lopes Pontes; Vasconcelos, Maria de Fátima Carvalho; Maia, Lucianne Cople; Tannure, Patrícia Nivoloni; Antônio, Andréa Gonçalves

doi:10.1007/s10006-015-0487-4

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…Biofilms can cause serious medical problems since they represent a reservoir of bacteria that can be shed into the body, leading to chronic infections [23]. Biofilms play a pivotal role in healthcare-associated infections, especially those related to the implant of medical devices, such as catheters, orthopaedic implants, artificial heart valves, suture threads also represent a good carrier for biofilm formation [15,24,25]. Two main mechanisms contribute to biofilm resistance: (1) prevention of the antibacterial substance from reaching its target, e.g., by limited diffusion or repulsion, and (2) the specific physiology of a biofilm, which limits the efficacy of antibiotics, mainly of those that target active cell processes, and which may also include specific subpopulations of resistant cells [6,26].…”

Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Caputo

Martino

Perfetto

et al. 2018

IJERPH

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For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis, combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis, thus helping to establish an effective antibiotic therapy.

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Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Caputo

Martino

Perfetto

et al. 2018

IJERPH

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“…Propensity of bacteria to adhere to braided and monofilament suture material was assessed using an in vitro adhesion assay (70). Briefly, 1-cm segments of sterile braided Mersilene or monofilament Ethilon were prepared with a sterile blade and tweezers.…”

Section: In Vitro Adhesion Assaymentioning

confidence: 99%

Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage

et al. 2016

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Preterm birth, the leading cause of death in children under 5 years, may be caused by inflammation triggered by ascending vaginal infection. About 2 million cervical cerclages are performed annually to prevent preterm birth. The procedure is thought to provide structural support and maintain the endocervical mucus plug as a barrier to ascending infection. Two types of suture material are used for cerclage: monofilament or multifilament braided. Braided sutures are most frequently used, although no evidence exists to favor them over monofilament sutures. We assessed birth outcomes in a retrospective cohort of 678 women receiving cervical cerclage in five UK university hospitals and showed that braided cerclage was associated with increased intrauterine death (15% versus 5%; P = 0.0001) and preterm birth (28% versus 17%; P = 0.0006) compared to monofilament suture. To understand the potential underlying mechanism, we performed a prospective, longitudinal study of the vaginal microbiome in women at risk of preterm birth because of short cervical length (≤25 mm) who received braided (n = 25) or monofilament (n = 24) cerclage under comparable circumstances. Braided suture induced a persistent shift toward vaginal microbiome dysbiosis characterized by reduced Lactobacillus spp. and enrichment of pathobionts. Vaginal dysbiosis was associated with inflammatory cytokine and interstitial collagenase excretion into cervicovaginal fluid and premature cervical remodeling. Monofilament suture had comparatively minimal impact upon the vaginal microbiome and its interactions with the host. These data provide in vivo evidence that a dynamic shift of the human vaginal microbiome toward dysbiosis correlates with preterm birth.

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“…Surgical wound repair is a complex physiological process that includes inflammation, cell proliferation, matrix deposition and tissue remodeling 1 . Sutures are materials of first choice for the synthesis of these surgical sites because they exert less influence in the healing stages and promote tissue repair by first intention 2 . The main problem with surgical sutures is the deposition of biofilm on the surface and direct contact with the wound, increasing the risk of surgical site infections and altering the quality of the scar tissue 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Surgical incisions in the oral cavity are even more vulnerable to infections, occurring in 2 to 12% of surgeries 2,4 . Some factors, such as the diversity of microorganisms, the presence of saliva, rich vascularization, contamination by food residues and trauma resulting from speech and hygiene increase the inflammatory process, the healing time and the discomfort of the patient 5 .…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, in vitro studies 2,3,10 , in vivo 11,12 and clinical trials 13,14 analyzed the adherence of bacteria present in the oral cavity in sutures. Moreover, there are not still studies that considered the adhesion of C. albicans and the antifungal effect of coatings on surgical sutures under conditions that simulate the oral environment.…”

Section: Introductionmentioning

confidence: 99%

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Sutures modified by incorporation of chlorhexidine and cinnamaldehyde: anti-Candida effect, bioavailability and mechanical properties

Costa

Cavalcanti

Valença

et al. 2019

Rev. odontol. UNESP

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Introduction Antimicrobial sutures are a therapeutic alternative for the control of oral infections. Objective Incorporate Chlorhexidine (CHX) and Cinnamaldehyde (CN) in sutures and evaluate the anti-Candida effect, release of antimicrobials and mechanical properties. Material and method Silk (S) and Polyglactin 910 (P) sutures were aseptically sectioned (20 mm) and immersed for incorporation in 0.12% CHX, 0.4% CN and 0.9% saline solutions under stirring for 60 minutes (n = 10 / group). Suspensions of 500 μL of Candida albicans (ATCC 90028/ 1 × 106 CFU/mL) were used to evaluate fungal adhesion after the 48 h period at 37°C. The release of CLX and CN were evaluated at 0, 24 and 48 hours (n=3/group) by UV-VIS spectrophotometer (275 nm). The tensile strength and displacement (n=5/group) were evaluated after incorporation (30 mm/min, 50N). Data were analyzed by Anova and Tukey (α = 5%). Result No anti-Candida effect was observed on S and P sutures incorporated with CLX and CN (p>0.05). However, progressive release was verified up to 48 after treatment with CLX (S = 0.075 / P = 0.073 μg/mL) and CN (S = 35.33 /P= 5.72 μg/mL). There was a decrease in tensile strength in S (CLX = 9.9 / CN = 9.9 N) and P (CLX = 14.4 / CN = 15.5 N) (p<0.05). No differences were observed for the displacement for S (CLX = 19.3 / CN=20.7 mm) and P (CLX = 16.2 / CN=15.8 mm) (p>0.05). Conclusion The incorporation of CLX and CN did not have a positive effect on the biological and mechanical properties of the sutures evaluated.

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Oral bacteria adherence to suture threads: an in vitro study

Abstract: Total microorganisms and P. intermedia have different affinities to the tested suture threads, whereas F. nucleatum presented a similar adherence level. Among the threads, nylon (G1), followed by polyglactin 910 with triclosan (G4) presented the lowest microbial adherence level.

Cited by 6 publications

References 32 publications

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage

Sutures modified by incorporation of chlorhexidine and cinnamaldehyde: anti-Candida effect, bioavailability and mechanical properties

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