Oral calcium pectinate-insulin nanoparticles: influences of alginate, sodium chloride and Tween 80 on their blood glucose lowering performance

Wong, Tin Wui; Sumiran, N.

doi:10.1111/jphp.12192

Cited by 11 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro drug release study showed undetectable levels of insulin release from the AN even after 6 h of incubation in the dissolution medium. The alginate has a p K a value of 3.65, while the amphoteric insulin molecule has an isoelectric point of 5.3 . The nanoparticles were prepared by mixing the negatively charged alginate (dissolved in NaOH) with the positively charged insulin (dissolved in HCl).…”

Section: Resultsmentioning

confidence: 99%

“…The alginate has a pK a value of 3.65, 47 while the amphoteric insulin molecule has an isoelectric point of 5.3. 48 The nanoparticles were prepared by mixing the negatively charged alginate (dissolved in NaOH) with the positively charged insulin (dissolved in HCl). Complex formation with the alginate would prevent the insulin from being released from the formed matrix.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Alginate–C18 Conjugate Nanoparticles Loaded in Tripolyphosphate-Cross-Linked Chitosan–Oleic Acid Conjugate-Coated Calcium Alginate Beads as Oral Insulin Carrier

2018

View full text Add to dashboard Cite

Simple alginate, alginate-stearic acid, and alginate-C18 conjugate nanoparticles and tripolyphosphate-cross-linked chitosan-oleic acid conjugate-coated calcium alginate beads as the vehicle of nanoparticles were designed. Their size, ζ potential, morphology, drug load, drug release, matrix molecular characteristics, mucus penetration, HT-29 cell line cytotoxicity and intracellular trafficking, in vivo blood glucose lowering, and insulin delivery profiles were characterized. Alginate-C18 conjugate nanoparticles were nontoxic. Among all nanoparticle variants, they had reduced size and ζ potential thus enhancing particulate mucus penetration and intracellular trafficking. Their insulin reabsorption tendency was minimized as alginate active COOH/COO- sites were preoccupied with C18. Their loading into coated beads was translated to reduced drug release in simulated gastric phase with nanoparticles being released in the intestinal phase. The combination dosage form increased the blood glucose lowering extent of insulin and blood insulin level compared with nanoparticles or beads alone. Nanoparticles in beads represented a viable approach for oral insulin delivery.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Alginate–C18 Conjugate Nanoparticles Loaded in Tripolyphosphate-Cross-Linked Chitosan–Oleic Acid Conjugate-Coated Calcium Alginate Beads as Oral Insulin Carrier

2018

View full text Add to dashboard Cite

show abstract

“…These are prepared from natural or synthetic polymers. Natural polymers studied for preparation and evaluation of oral insulin nanoparticles include chitosan (Lin et al, 2007b;Sadeghi et al, 2008;Rekha and Sharma, 2009;Yin et al, 2009;Avadi et al, 2010;Su et al, 2012;Fonte et al, 2012;Chuang et al, 2013;Li et al, 2013b;Mansourpour et al, 2015), alginate (Kadir et al, 2013;Wong and Sumiran, 2014), gelatin, albumin (Rieux et al, 2006;Woitiski et al, 2011) and lectin (Ghilzai, 2003). Synthetic polymers used for nanoparticle formulation include acrylates and its derivatives (Sajeesh and Sharma, 2006;Damge et al, 2010;Perera et al, 2009;Socha et al, 2009), polylactic-co-glycolic acid derivatives (Carino et al, 2000;Shi et al, 2008;Han et al, 2009;Zhu et al, 2015).…”

Section: Nanoparticles Developed For Oral Delivery Of Insulinmentioning

confidence: 99%

Oral Delivery of Insulin for Treatment of Diabetes: Classical Challenges and Current Opportunities

Ansari¹

2015

J. of Medical Sciences

View full text Add to dashboard Cite

show abstract

“…[23][24][25] Calcium pectinate-insulin NPs formed from a mixture of pectin-insulin at pH 3 were associated with a release of 12.6% ± 3.2% and 21.7% ± 8.7% insulin at 8 and 24 h of dissolution in simulated intestinal medium due to the sustained-release characteristics effect of pectin-insulin interaction. 26 Oral administration of zinc oxide NPs [ Figure 1] also resulted in signifi cant antidiabetic effects -with improved glucose tolerance, higher serum insulin (70%), reduced blood glucose (29%), reduced non-esterifi ed fatty acids (40%) and reduced triglycerides (48%). 27 Insulin should be enveloped in a matrix-like system to protect it from gastric enzymes.…”

Section: Oral Insulinmentioning

confidence: 99%

Nanoparticles and the new era in diabetes management

Woldu

Lenjisa

2014

Int J Basic Clin Pharmacol

View full text Add to dashboard Cite

Diabetes mellitus (DM) has been known to mankind for more than 2000 years. DM is a group of metabolic disorder characterized by a complete lack of insulin, a relative lack of insulin, or insulin resistance. The increase in prevalence of DM is due to three infl uences: lifestyle, ethnicity, and age. Current challenges in diabetes management include: optimizing the use of the already available therapies to ensure adequate glycemic, blood pressure, and lipid control and to reduce complications. At present several researches have been focusing on new management options for diabetes. Among these options the use of nanomedicine is becoming an eye catching and most promising. The aim of the present review is to provide brief overview of the applications of nanoparticles (NPs) in diabetes management. The development of improved oral insulin administration is very essential for the treatment of DM to overcome the problem of daily subcutaneous injections. In diabetic patients oral administration of insulin can be benefi cial not only to alleviate the pain and trauma caused by injections, but it can also mimic the physiological fate of insulin as well. It has been found that NPs of chitosan, calcium pectinate zinc oxide, alginate, casein and different polymers have been used as a carrier for oral insulin delivery. Buccal administration of insulin with absorption enhancers showed a maximum 12% pharmacological activity. Biodegradable Polymeric NPs for parenteral insulin delivery have also been used, where the insulin matrix surrounded by the nanoporous membrane containing grafted glucose oxidase. A rise in blood glucose level triggers a change in the surrounding nanoporous membrane, resulting in biodegradation and subsequent insulin delivery. Inhalable, polymeric NP-based drug delivery systems have also been tried earlier for the treatment of tuberculosis and cardiovascular disease treatment. Such approaches can be directed toward insulin delivery through inhalable NPs. All previous studies resulted in post treatment accumulation of the NPs in skin and eyes. These drug delivery technologies are in various stages of research and development. The medical applications for nanotechnology are enormous and could give medicine, including the treatment of diabetes, an entirely new outlook.

show abstract

Oral calcium pectinate-insulin nanoparticles: influences of alginate, sodium chloride and Tween 80 on their blood glucose lowering performance

Abstract: Physicochemical responses of additives in vivo affected blood glucose regulation property of pectin-insulin nanoparticles.

Cited by 11 publications

References 25 publications

Alginate–C18 Conjugate Nanoparticles Loaded in Tripolyphosphate-Cross-Linked Chitosan–Oleic Acid Conjugate-Coated Calcium Alginate Beads as Oral Insulin Carrier

Alginate–C18 Conjugate Nanoparticles Loaded in Tripolyphosphate-Cross-Linked Chitosan–Oleic Acid Conjugate-Coated Calcium Alginate Beads as Oral Insulin Carrier

Oral Delivery of Insulin for Treatment of Diabetes: Classical Challenges and Current Opportunities

Nanoparticles and the new era in diabetes management

Contact Info

Product

Resources

About