Oral Fungal Alterations in Patients with COVID‐19 and Recovered Patients

Hu, Xiaobo; Li, Ang; Yu, Bo; Yu, Jia; Lü, Haifeng; Sun, Junyi; Sun, Ying; Zou, Yawen; Luo, Hong; Zeng, Zhaohai; Liu, Shanshuo; Jiang, Yan; Wang, Zhongwen; Ren, Zhigang

doi:10.1002/advs.202205058

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…The oral microbiome is the second largest and most diverse microecosystem of up to approximately 1000 microbial species, which is crucial in maintaining oral as well as systemic health ( Deo and Deshmukh, 2019 ). Emerging studies have shown that oral microbiota is associated with various diseases ( Hayes et al., 2018 ; Radaic and Kapila, 2021 ; Hu et al., 2023 ; Lacunza et al., 2023 ), and can be used as a diagnostic tool for specific diseases or cancer, such as rheumatoid arthritis ( Zhang et al., 2015 ) and pancreatic cancer ( Vogtmann et al., 2020 ). Our previous research has verified the functional significance of the human tongue microbiota in COVID-19 ( Ren et al., 2021 ) and SLE ( Guo et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Oral microbiota distinguishes patients with osteosarcoma from healthy controls

Chen,

Li,

Wang

et al. 2024

Front. Cell. Infect. Microbiol.

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ObjectiveThe human microbiota plays a key role in cancer diagnosis, pathogenesis, and treatment. However, osteosarcoma-associated oral microbiota alterations have not yet been unraveled. The aim of this study was to explore the characteristics of oral microbiota in osteosarcoma patients compared to healthy controls, and to identify potential microbiota as a diagnostic tool for osteosarcoma.MethodsThe oral microbiota was analyzed in osteosarcoma patients (n = 45) and matched healthy controls (n = 90) using 16S rRNA MiSeq sequencing technology.ResultsThe microbial richness and diversity of the tongue coat were increased in osteosarcoma patients as estimated by the abundance-based coverage estimator indices, the Chao, and observed operational taxonomy units (OTUs). Principal component analysis delineated that the oral microbial community was significant differences between osteosarcoma patients and healthy controls. 14 genera including Rothia, Halomonas, Rhodococcus, and Granulicatella were remarkably reduced, whereas Alloprevotella, Prevotella, Selenomonas, and Campylobacter were enriched in osteosarcoma. Eventually, the optimal four OTUs were identified to construct a microbial classifier by the random forest model via a fivefold cross-validation, which achieved an area under the curve of 99.44% in the training group (30 osteosarcoma patients versus 60 healthy controls) and 87.33% in the test group (15 osteosarcoma patients versus 30 healthy controls), respectively. Notably, oral microbial markers validated strong diagnostic potential distinguishing osteosarcoma patients from healthy controls.ConclusionThis study comprehensively characterizes the oral microbiota in osteosarcoma and reveals the potential efficacy of oral microbiota-targeted biomarkers as a noninvasive biological diagnostic tool for osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

Oral microbiota distinguishes patients with osteosarcoma from healthy controls

Chen,

Li,

Wang

et al. 2024

Front. Cell. Infect. Microbiol.

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Oral Fungal Alterations in Patients with COVID‐19 and Recovered Patients

et al. 2023

Advanced Science

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The oral bacteriome, gut bacteriome, and gut mycobiome are associated with coronavirus disease 2019 (COVID‐19). However, the oral fungal microbiota in COVID‐19 remains unclear. This article aims to characterize the oral mycobiome in COVID‐19 and recovered patients. Tongue coating specimens of 71 COVID‐19 patients, 36 suspected cases (SCs), 22 recovered COVID‐19 patients, 36 SCs who recovered, and 132 controls from Henan are collected and analyzed using internal transcribed spacer sequencing. The richness of oral fungi is increased in COVID‐19 versus controls, and beta diversity analysis reveals separate fungal communities for COVID‐19 and control. The ratio of Ascomycota and Basidiomycota is higher in COVID‐19, and the opportunistic pathogens, including the genera Candida, Saccharomyces, and Simplicillium, are increased in COVID‐19. The classifier based on two fungal biomarkers is constructed and can distinguish COVID‐19 patients from controls in the training, testing, and independent cohorts. Importantly, the classifier successfully diagnoses SCs with positive specific severe acute respiratory syndrome coronavirus 2 immunoglobulin G antibodies as COVID‐19 patients. The correlation between distinct fungi and bacteria in COVID‐19 and control groups is depicted. These data suggest that the oral mycobiome may play a role in COVID‐19.

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Oral Microbiota Alterations in Subjects with SARS-CoV-2 Displaying Prevalence of the Opportunistic Fungal Pathogen Candida albicans

Alfaifi,

Holm,

Wang

et al. 2024

Microorganisms

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The oral cavity remains an underappreciated site for SARS-CoV-2 infection despite the myriad of oral conditions in COVID-19 patients. Recently, SARS-CoV-2 was shown to replicate in the salivary gland cells causing tissue inflammation. Given the established association between inflammation and microbiome disruption, we comparatively profiled oral microbial differences at a metagenomic level in a cohort of hospitalized COVID-19 patients and matched healthy controls. Specifically, we aimed to evaluate colonization by the opportunistic fungal pathogen Candida albicans, the etiologic agent of oral candidiasis. Comprehensive shotgun metagenomic analysis indicated that, overall, COVID-19 patients exhibited significantly reduced bacterial and viral diversity/richness; we identified 12 differentially abundant bacterial species to be negatively associated with COVID-19, and the functional pathways of certain bacteria to be highly associated with COVID-19 status. Strikingly, C. albicans was recovered from approximately half of the COVID-19 subjects but not from any of the healthy controls. The prevalence of Candida is likely linked to immune hypo-dysregulation caused by COVID-19 favoring Candida proliferation, warranting investigations into the interplay between Candida and SARS-CoV2 and potential therapeutic approaches directed toward oral candidiasis. Collectively, our findings prompt a reassessment of oral opportunistic infection risks during COVID-19 disease and their potential long-term impacts on oral health.

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Oral Fungal Alterations in Patients with COVID‐19 and Recovered Patients

Cited by 3 publications

References 40 publications

Oral microbiota distinguishes patients with osteosarcoma from healthy controls

Oral microbiota distinguishes patients with osteosarcoma from healthy controls

Oral Fungal Alterations in Patients with COVID‐19 and Recovered Patients

Oral Microbiota Alterations in Subjects with SARS-CoV-2 Displaying Prevalence of the Opportunistic Fungal Pathogen Candida albicans

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