Oral gram-positive bacterial DNA-based identification of saliva from highly degraded samples

Ohta, Jun; Sakurada, Koichi

doi:10.1016/j.fsigen.2019.06.016

Cited by 17 publications

(11 citation statements)

References 38 publications

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“…At present, it would seem that further studies are necessary before microRNA and DNA methylation methods can be applied to practical work. Meanwhile, studies focusing on bacterial DNA such as Streptococcus salivarius, which parasitizes in the oral cavity, have also increased [57][58][59][60]. Such methods include the development of a simple and rapid method involving the loop-mediated isothermal amplification (LAMP) technique, as well as a useful method for deteriorated salivary stain samples.…”

Section: Saliva Identificationmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Section: Saliva Identificationmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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“…The possibility of final classification was investigated by applying our classification method to the selected sample using the strain reported as the major microbiome component at the species level. The strains observed in the gargle samples were Streptococcus salivarius , S. sanguinis , and Neisseria subflava [ 31 ], whereas those in the urine samples were Lactobacillus spp. and Gardnerella vaginalis [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Individual Identification with Short Tandem Repeat Analysis and Collection of Secondary Information Using Microbiome Analysis

Lee

You

Lee

et al. 2021

Genes

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Forensic investigation is important to analyze evidence and facilitate the search for key individuals, such as suspects and victims in a criminal case. The forensic use of genomic DNA has increased with the development of DNA sequencing technology, thereby enabling additional analysis during criminal investigations when additional legal evidence is required. In this study, we used next-generation sequencing to facilitate the generation of complementary data in order to analyze human evidence obtained through short tandem repeat (STR) analysis. We examined the applicability and potential of analyzing microbial genome communities. Microbiological supplementation information was confirmed for two of four failed STR samples. Additionally, the accuracy of the gargle sample was confirmed to be as high as 100% and was highly likely to be classified as a body fluid sample. Our experimental method confirmed that anthropological and microbiological evidence can be obtained by performing two experiments with one extraction. We discuss the advantages and disadvantages of using these techniques, explore prospects in the forensic field, and highlight suggestions for future research.

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“…Age, gender, and race are also determined by protein profiling and evaluating the total salivary protein concentration using the standard baseline of the protein variations [ 119 ]. Screening the α -amylase activity is a sensitive, simple, and cost-effective method for indicating the saliva presence; however, it is low specific [ 120 ]. Then, preserving the α -amylase stability is a must for catalytic and immunological forensic saliva investigations [ 121 ].…”

Section: Medical Biotechnology Techniquesmentioning

confidence: 99%

“…Different eating patterns, oral hygiene measures, humidity, climate, temperature, or even disease outbreaks can be used for recognizing various geographical locations since any locales result in a different salivary microbial community in composition and function [ 117 ]. Methods based on various oral resident bacteria such as Streptococcus salivarius , Streptococcus mutans , and Veillonella atypica are more specific than protein-based methods [ 120 ]. One of the most important species in forensic microbiology is S. mutans which is substituted in the mouth during birth and remain there throughout life.…”

Section: Medical Biotechnology Techniquesmentioning

confidence: 99%

Dental Radiographic/Digital Radiography Technology along with Biological Agents in Human Identification

et al. 2022

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The heavy casualties associated with mass disasters necessitate substantial resources to be managed. The unexpectedly violent nature of such occurrences usually remains a problematic amount of victims that urgently require to be identified by a reliable and economical method. Conventional identification methods are inefficient in many cases such as plane crashes and fire accidents that have damaged the macrobiometric features such as fingerprints or faces. An appropriate recognition method for such cases should use features more resistant to destruction. Forensic dentistry provides the most appropriate available method for the successful identification of victims using careful techniques and precise data interpretation. Since bones and teeth are the most persistent parts of the demolished bodies in sudden mass disasters, scanning and radiographs are unrepeatable parts of forensic dentistry. Forensic dentistry as a scientific method of human remain identification has been considerably referred to be efficient in disasters. Forensic dentistry can be used for either “sex and age estimation,” “Medical biotechnology techniques,” or “identification with dental records,” etc. The present review is aimed at discussing the development and implementation of forensic dentistry methods for human identification. For this object, the literature from the last decade has been searched for the innovations in forensic dentistry for human identification based on the PubMed database.

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Oral gram-positive bacterial DNA-based identification of saliva from highly degraded samples

Cited by 17 publications

References 38 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Individual Identification with Short Tandem Repeat Analysis and Collection of Secondary Information Using Microbiome Analysis

Dental Radiographic/Digital Radiography Technology along with Biological Agents in Human Identification

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