Oral keratinocytes cultured on dermal matrices form a mucosa-like tissue

In vivo behavior of complete human oral mucosa equivalents: characterization in athymic mice

“…In the third week after grafting, our equivalents featured an epithelium organized into three distinct layers, each showing a proper cell morphology similar to what would be expected from a nonkeratinized oral mucosa (15). Our work highlights the possibility of achieving in vivo the same results other authors only obtain in vitro (16,17). Furthermore, by employing submerged CAOMEs instead of air–liquid interface differentiated CAOMEs (12,13), we did not observe any hydropic degeneration or epithelial loss at 21 d after grafting, which are issues alluded to in the cited studies.…”

Cleft Palate Cells Can Regenerate a Palatal Mucosa in vitro

“…Several researchers have constructed substitutes of normal palatal mucosa using de-epidermized connective tissue or collagen scaffolds to solve this problem Ophof et al, 2002). In the present study, from children with cleft palate and from age-matched individuals, mucosal substitutes were constructed with keratinocytes and fibroblasts cultured on de-epidermized connective tissue.…”

Tissue-engineered Oral Mucosa: a Review of the Scientific Literature

“…(3) a stratified squamous epithelium on the basement membrane, including densely packed keratinocytes that undergo differentiation as they migrate to the surface. This may be mimicked by the growth in culture of oral keratinocytes at the air-liquid interface in a chemically defined medium, which contains keratinocyte growth factors such as epidermal growth factor (EGF) (Izumi et al, 1999;Moriyama et al, 2001;Ophof et al, 2002;Rouabhia and Deslauriers, 2002;Bhargava et al, 2004). Significant issues that need to be addressed in the growth of multilayered epithelial constructs on connective tissue substrates include keratinocyte invasion into the connective tissue layer and poor differentiation of the epithelium.…”